Analysis of miRNA-mediated regulation of telomerase and application for cervical cancer gene therapy

• Project/Area Number: 20591940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa University
• Principal Investigator: TAKAKURA Masahiro Kanazawa University, 附属病院, 助教 (20313661)
• Co-Investigator(Kenkyū-buntansha): KYO Satoru 金沢大学, 医学系, 講師 (50272969)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マイクロRNA / テロメラーゼ / 子宮頚癌 / 癌

Research Abstract

miRNA miR299-3p is involved in the regulation of telomerase. The degradation of hTERT, the catalytic component of telomerase, mRNA was accelerated by miR299-3p. The decrease of miR299-3p in cervical cancer tissue may be involved in activation of telomerase.

Research Products

• [Journal Article] Activation of NF-κB is a novel target of KRAS-induced endometrial carcinogenesis. (2011)
  • Author(s): Mizumoto Y, Kyo S, Kiyono T, Takakura M, Nakamura M, Maida Y, Mori N, Bono Y, Sakurai H, Inoue M
  • Journal Title: Clin.Cancer Res. 17 Pages: 525-537
  • Peer Reviewed
• [Journal Article] Activation of NF-KB is a novel target of KRAS-induced endometrial carcinogenesis. (2011)
  • Author(s): Mizumoto Y, Kyo S, Takakura M, et al.
  • Journal Title: Clin.Cancer Res. Volume: 17 Pages: 1341-50
  • Peer Reviewed
• [Journal Article] Intraperitoneal administration of telomerase-specific oncolytic adenovirus sensitizes ovarian cancer cells to cisplatin and affects survival in a xenograft model with peritoneal dissemination. (2010)
  • Author(s): Takakura M, Nakamura M, Kyo S, Hashimoto M, Mori N, Ikoma T, Mizumoto Y, Fujiwara T, Urata Y, Inoue M
  • Journal Title: Cancer Gene Ther. 17 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Expression of HER-2 impacts patient survival and paclitaxel sensitivity in endometrial cancer. (2010)
  • Author(s): Mori N, Kyo S, Nakamura M, Hashimoto M, Maida Y, Mizumoto Y, Takakura M, Ohno S, Kiyono T, Inoue M
  • Journal Title: Br.J.Cancer. 103 Pages: 889-898
  • Peer Reviewed
• [Journal Article] Prognostic impact of CD133 expression as a tumor intiating marker in endometrial (2010)
  • Author(s): Nakamura M, Kyo S, Zhang B, ZhnagX, Mizumoto Y, Takakura M, Maida Y, Mori N, Hashimoto M, Ohno S, Inoue M
  • Journal Title: Hum.Pathol. 41 Pages: 1516-1529
  • Peer Reviewed
• [Journal Article] Prognostic impact of CD133 expression as a tumor initiating marker inendometrial cancer. (2010)
  • Author(s): Nakamura M, Kyo S, Takakura M, et al.
  • Journal Title: Hum.Pathol. Volume: 41 Pages: 1516-29
  • Peer Reviewed
• [Journal Article] Expression of HER-2 impacts patient survival and paclitaxel sensitivityin endometrial cancer (2010)
  • Author(s): Mori N, Kyo S, Takakura M, et al.
  • Journal Title: Br.J.Cancer Volume: 103 Pages: 889-98
  • Peer Reviewed
• [Journal Article] Diagnostic potential and limitation of imaging cancer cell in cytological sample using telomerase-specific replicative adenovirus. (2009)
  • Author(s): Maida Y, Kyo S, Sakaguchi J, Mizumoto Y, Hashimoto M, Mori M, Ikoma T, Nakamura M, Takakura M, Urata Y, Fujiwara T, Inoue M
  • Journal Title: Int.J.Oncol. 34 Pages: 1549-1556
  • Peer Reviewed
• [Journal Article] Ikoma T, Kyo S, Maida Y, Ozaki S, Takakura M, Nakao S, Inoue M (2009)
  • Author(s): Ikoma T, Kyo S, Maida Y, Ozaki S, Takakura M, Nakao S, Inoue M
  • Journal Title: Am J Obstet Gynecol. 201 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Intraperitoneal administration of telomerase-specific oncolytic adenovirus sensitizes ovarian cancer cells to cisplatin and affects survival in a xenograft model with peritoneal dissemination (2009)
  • Author(s): Takakura M, Nakamura M, Kyo S., et.al.
  • Journal Title: Cancer Gene Ther. 17 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Bone marrow-derived cells from male donors can compose endometrial glands in female transplant recipients (2009)
  • Author(s): Ikoma T, Kyo S, Takakura M, et.al.
  • Journal Title: Am J Obstet Gynecol. 201
  • Peer Reviewed
• [Journal Article] Diagnostic potential and limitation of imaging cancer cells in cytological samples using telomerase-specific replicative adenovirus (2009)
  • Author(s): Maida Y, Kyo S, Takakura M, et.al.
  • Journal Title: Int.J Oncol. 34 Pages: 1549-1556
  • Peer Reviewed
• [Journal Article] Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers. (2008)
  • Author(s): Kyo S, Takakura M, Fujiwara T, Inoue M
  • Journal Title: Cancer Sci. 9 Pages: 1528-1538
  • NAID: 10024009430
  • Peer Reviewed
• [Journal Article] Role of menin in the regulation of telomerase activity in normal and cancer cells. (2008)
  • Author(s): Hashimoto M, Kyo S, Hua X, Tahara H, Nakajima M, Takakura M, Saskaguchi J, Maida Y, Nakamura M, Ikoma T, Mori N, Mizumoto Y, Inoue M
  • Journal Title: Int.J.Oncol. 33 Pages: 333-340
  • Peer Reviewed
• [Journal Article] 卵巣癌の遺伝子治療 (2008)
  • Author(s): 高倉正博, 京哲, 井上正樹
  • Journal Title: 臨床婦人科産科 62 Pages: 1347-1351
• [Journal Article] Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers. (2008)
  • Author(s): Kyo S, Takakura M, et. al.
  • Journal Title: Cancer Sci. 99 Pages: 1528-1538
  • NAID: 10024009430
  • Peer Reviewed
• [Journal Article] Role of menin in the regulation of telomerase activity in normal and cancer cells. (2008)
  • Author(s): Hashimoto M, Takakura M, et. al.
  • Journal Title: Int. J. Oncol. 33 Pages: 333-340
  • Peer Reviewed
• [Journal Article] 卵巣癌の遺伝子治療 (2008)
  • Author(s): 高倉正博, 京 哲, 井上正樹
  • Journal Title: 産科と婦人科 62 Pages: 1347-52
• [Presentation] microRNAによるテロメラーゼ制御機構の解明と婦人科発癌および細胞老化への影響の解明 (2009)
  • Author(s): 高倉正博
  • Organizer: 財団法人神澤医学研究振興財団第11回講演会(ホテルオークラ)
  • Place of Presentation: 東京都
  • Year and Date: 2009-05-29
• [Presentation] microRNAによるテロメラーゼ制御機構の解明と婦人科癌発癌および細胞老化への影響の解明 (2009)
  • Author(s): 高倉正博
  • Organizer: 財団法人神澤医学研究振興財団 第11回講演会
  • Place of Presentation: ホテルオークラ (東京都)
  • Year and Date: 2009-05-29
• [Presentation] microRNA mediated regulation of telomerase in cervical cancer (2008)
  • Author(s): 高倉正博, 京哲, 中村充宏, 橋本学, 森紀子, 水本泰成, 生駒友美, 井上正樹
  • Organizer: 第67回日本癌学会
  • Place of Presentation: (名古屋国際会議場)愛知県
  • Year and Date: 2008-10-28
• [Presentation] micro RNA mediated regulation of telomerase in cervical cancer (2008)
  • Author(s): 高倉正博
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] 子宮頚癌におけるテロメラーゼ制御にはmiRNAが関与している (2008)
  • Author(s): 高倉正博, 京哲, 中村充宏, 橋本学, 森紀子, 水本泰成, 生駒友美, 坂口純子, 井上正樹
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: (パシフィコ横浜)神奈川県
  • Year and Date: 2008-04-12
• [Presentation] 子宮頚癌におけるテロメラーゼ制御にはmiRNAが関与している (2008)
  • Author(s): 高倉 正博
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-12
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