ovarian cancer-specific vaccine therapy by carrier cell

• Project/Area Number: 20591952
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Ehime University
• Principal Investigator: HAMADA Katsuyuki Ehime University, 医学部附属病院, 講師 (90172973)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 婦人科腫瘍学 / 遺伝子治療 / ワクチン療法 / 卵巣癌 / キャリアー細胞 / GM-CSF / オンコリティックアデノウイルス / プロモーター / IAI.3B

Research Abstract

GFP-introduced non small cell lung cancer A549 cells infected with oncolytic adenovirus, AdE3-IAI.3B was injected into the mouse subcutaneous tumors and DNA contents of GFP and AdE3-IAI.3B in the tumors peaked one day later and disappeared two weeks later. EHK-2 cell was established from A549 cells by limiting dilution and showed the 10 time higher antitumor effect than the original A549 cells. OVHM subcutaneous tumor formation was rejected in 40% of mice after the subcutaneous injection of AdE3-IAI.3B-infected EHK-2 carrier cells. This indicated that xenogenic antitumor immunity was introduced after the injection of carrier cells. The infection condition of carrier cells at 200MOI (multiplicity of infection) and 33 hours showed the best antitumor effect. The morphology of non-infected carrier cells was not changed but AdE3-IAI.3B-infected carrier cells showed the nuclear membrane lobulation. After the freeze and thaw of carrier cells, the lobulated nuclear membrane was ruptured and the adenovirus particles were released from the nucleus into the cytoplasm. Under these infection conditions, the in vitro antitumor effect increased by 20% and 90% of mouse showed the complete tumor reduction after the prior immunization of adenovirus. In the mouse model of intraperitoneal dissemination of OVHM ovarian cancer cells, the intraperitoneal injection of AdE3-IAI.3B-infected carrier cells did not cure any mice but the carrier cells infected with Ad-mGM-CSF and AdE3-IAI.3B showed the complete tumor reduction in all treated mice after the prior adenoviral immunization. The tumor formation of OVHM ovarian cancer cells was rejected in all complete tumor reduced mice treated with carrier cells and self-antitumor immunity was introduced in these mice.

Research Products

• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2011)
  • Author(s): Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K., Hamakawa, H.
  • Journal Title: Oncology Reports 25 Pages: 795-802
  • Peer Reviewed
• [Journal Article] DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice. (2010)
  • Author(s): Ito, T., Yoshihara, C., Hamada, K., Koyama, Y.
  • Journal Title: Biomaterials 31 Pages: 2912-2918
  • Peer Reviewed
• [Journal Article] Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings : Therapeutic effect on metastatic ovarian cancer (2010)
  • Author(s): Yoshihara, C., Hamada, K., Koyama, Y.
  • Journal Title: Oncology Reports. 23 Pages: 733-738
  • Peer Reviewed
• [Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter-driven oncolytic adenovirus. (2010)
  • Author(s): Hamada K., Zhang T., Desaki J., Nakashiro K., Ito H., Tani K., Koyama Y., Hamakawa H.
  • Journal Title: Journal of Gene Medicine 12 Pages: 545-554
  • Peer Reviewed
• [Journal Article] DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice. (2010)
  • Author(s): Ito, T., Yoshihara, C., Hamada, K., Koyama, Y.
  • Journal Title: Biomaterials Volume: 31 Pages: 2912-2918
  • Peer Reviewed
• [Journal Article] Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings : Therapeutic effect on metastatic ovarian cancer. (2010)
  • Author(s): Yoshihara, C., Hamada, K., Koyama, Y.
  • Journal Title: Oncology Reports Volume: 23 Pages: 733-738
  • Peer Reviewed
• [Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter-driven oncolytic adenovirus. (2010)
  • Author(s): Hamada K., Zhang T., Desaki J., Nakashiro K., Ito H., Tani K., Koyama Y., Hamakawa H.
  • Journal Title: Journal of Gene Medicine Volume: 12 Pages: 545-554
  • Peer Reviewed
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2010)
  • Author(s): Zhang, T., Hamada, K., Hyodo, M., Itoh, H., Tani, K., Goda, H., Nakashiro, K., Hamakawa, H.
  • Journal Title: Oncology Reports Volume: 25 Pages: 795-802
  • Peer Reviewed
• [Journal Article] Improved gene transfer into renal carcinoma cells using adenovirus vector containing RGD motif. (2009)
  • Author(s): Terao, S., Acharya, B., Suzuki, T., Aoi, T., Naoe, M., Hamada, K., Mizuguchi, H. Gotoh, A.
  • Journal Title: Anticancer research 29 Pages: 2997-3002
  • Peer Reviewed
• [Journal Article] Preclinical test of oncolytic adenovirus-infected carrier cells for ovarian cancer (2009)
  • Author(s): Katsuyuki Hamada, Ting Zhang, Wenlin Huang
  • Journal Title: Molecular Therapy 17
  • Peer Reviewed
• [Journal Article] Preparation of polymer-coated adenovirus particles and their therapeutic effects in mouse tumor models (2009)
  • Author(s): Yoshiyuki Koyama, Chieko Yoshihara, Katsuyuki Hamada
  • Journal Title: Molecular Therapy 17
  • Peer Reviewed
• [Journal Article] Improved gene transfer into renal carcinoma cells using adenovirus vector containing RGD motif (2009)
  • Author(s): Shuji Terao, Bishnu Acharya, Toru Suzuki, Takashi Aoi, Michio Naoe, Katsuyuki Hamada, Hiroyuki Mizuguchi, Akinobu Gotoh
  • Journal Title: Anticancer Research 29 Pages: 2997-3002
  • Peer Reviewed
• [Journal Article] Gene therapy for solid tumors by A549 carrier cells infected with oncolytic adenovinis driven by IAI. 3B promoter. (2008)
  • Author(s): Katsuyuki Hamada, Ting Zhang, Yuka Hattori, Tetsuya Sawazaki, Michihiro Sumida
  • Journal Title: Molecular Therapy 16
  • Peer Reviewed
• [Presentation] Improved gene transfer into renal carcinoma cells using an adenovirus vector. (2010)
  • Author(s): Terao S., Acharya, B., Suzuki T., Hamada K., Mizuguchi, H., Gotoh A.
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Marriot Wardman Park Hotel Washington DC, USA.
• [Presentation] Gene therapy for head and neck squamous carcinoma cells by IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2010)
  • Author(s): Hamada K., Zhang,T., Nakashiro, K., Hamakawa, H.
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Marriot Wardman Park Hotel, Washington DC, USA.
• [Presentation] Small plasmid/PEI/anionic polysaccharide ternary complex particles : toxicity and animal clinical study. (2010)
  • Author(s): Koyama Y., Tojyo, M., Yoshihara C., Hamada K., Ito T.
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Marriot Wardman Park Hotel, Washington DC, USA.
• [Presentation] 超微細粒子化コンドロイチン硫酸-被覆DNA複合体を用いた新たなDDSによる癌特異的免疫遺伝子治療 (2010)
  • Author(s): 濱田雄行, 伊藤智子、芳原智恵子、小山義之
  • Organizer: 第62回日本産婦人科学会総会
  • Place of Presentation: 東京
• [Presentation] 人工エンベロープを持つ腫瘍溶解性アデノウィルスの調製とその卵巣腫瘍腹腔内転移モデルにおける遺伝子治療効果 (2010)
  • Author(s): 小山義之, 芳原智恵子、濱田雄行
  • Organizer: 第62回日本産婦人科学会総会
  • Place of Presentation: 東京
• [Presentation] Preclinical biosafety test of oncolytic adenovirus-infected carrier cell for phase I study. (2010)
  • Author(s): Hamada K., Zhang, T., Shirakawa T., Huang, W.
  • Organizer: 101th annual meeting of American society of cancer research(AACR)
  • Place of Presentation: Washington DC, USA.
• [Presentation] Improved gene transfer into renal carcinoma cells using an adenovirus vector. (2010)
  • Author(s): Shuji Terao, Bishnu Acharya, Toru Suzuki, Katsuyuki Hamada, Hiroyuki Mizuguchi, Akinobu Gotoh.
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Washington DC
• [Presentation] Gene therapy for head and neck squamous carcinoma cells by IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2010)
  • Author(s): Katsuyuki Hamada, Ting Zhang, Koh-ichi Nakashiro, Hiroyuki Hamakawa
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Washington DC
• [Presentation] Small plasmid/PEI/anionic polysaccharide ternary complex particles : toxicity and animal clinical study. (2010)
  • Author(s): Yoshiyuki Koyama, Masahiko Tojyo, Chieko Yoshihara, Katsuyuki Hamada, Tomoko Ito.
  • Organizer: 13rd Annual meeting of American society of gene and cell therapy (ASGCT)
  • Place of Presentation: Washington DC
• [Presentation] Preclinical biosafety test of oncolytic adenovirus-infected carrier cell for phase I study. (2010)
  • Author(s): Katsuyuki Hamada, Ting Zhang, Toshiro Shirakawa, Wenlin Huang.
  • Organizer: 101th annual meeting of American society of cancer research(AACR)
  • Place of Presentation: Washington DC
• [Presentation] 腫瘍免疫遺伝子治療動物医薬の開発とヒト治療薬への展開 (2009)
  • Author(s): 小山義之、濱田雄行
  • Organizer: 第9回バイオビジネスコンペJAPAN
  • Place of Presentation: シティプラザ大阪、大阪市
  • Year and Date: 2009-02-02
• [Presentation] Preclinical test of oncolytic adenovirus-infected carrier cells for ovarian cancer. (2009)
  • Author(s): Hamada K., Zhang,T., Huang, W.
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: San Diego, USA.
• [Presentation] Preparation of polymer-coated adenovirus particles and their therapeutic effects in mouse tumor models. (2009)
  • Author(s): 小山義之, 芳原智恵子, 濱田雄行
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: San Diego, USA.
• [Presentation] Intravenous injection of allogeneic tumor cell vaccine carrying oncolytic adenovirus (AdE3-IAI.3B) suppresses the growth of mMultiple lung tumors in mouse squamous cell carcinoma model. (2009)
  • Author(s): Shirakawa T., Hamada K., Kitajima K., Mitsuoka C., Morishita N., Yamaoka N., Saito A., Matsuoka T., Adhim Z., Terao S., Gotoh A., Nibu K., Fujisawa M., Lee K., Huang W.
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: San Diego, USA.
• [Presentation] High level therapeutic effects of fine plasmid/PEI/Hyaluronic acid ternary complex particles in tumor model mouse. (2009)
  • Author(s): 伊藤智子, 濱田雄行, 芳原智恵子, 小山義之
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: San Diego, USA.
• [Presentation] Improved gene transfer into renal carcinoma cells using an adenovirus vector. (2009)
  • Author(s): Terao S., Acharya, A., Suzuki T., Hamada K., Mizuguchi, H., Gotoh A.
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: San Diego
• [Presentation] Pharmacological and biosafety test of cancer gene therapy by oncolytic adenovirus-infected carrier cell. (2009)
  • Author(s): 濱田雄行
  • Organizer: 100th AACR annual meeting 2009.
  • Place of Presentation: Denver, USA.
• [Presentation] Polyanion-coated nanoparticleを用いた新たなDDSによる癌特異的免疫遺伝子治療 (2009)
  • Author(s): 濱田雄行, 伊藤智子、芳原智恵子、小山義之
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
• [Presentation] 自己会合性ポリペプチドゲルを用いた徐放型遺伝子導入注射製剤の開発 (2009)
  • Author(s): 奥冨さやか, 芳原智恵子, 伊藤智子, 民輪雅美, 高村健太郎, 濱田雄行, 小山義之
  • Organizer: 日本DDS学会
  • Place of Presentation: 東京
• [Presentation] キャリアー細胞を用いたオンコリティックアデノウイルスによる細胞性免疫性癌遺伝子治療の開発 (2009)
  • Author(s): 濱田雄行
  • Organizer: 第68回日本癌学会
  • Place of Presentation: 横浜
• [Presentation] 人工エンベロープを持つハイブリッド型アデノウィルスの調製とその遺伝子治療効果 (2009)
  • Author(s): 芳原智恵子, 濱田雄行, 小山義之
  • Organizer: 第68回日本癌学会
  • Place of Presentation: 横浜
• [Presentation] キャリアー細胞を用いた細胞性免疫性癌遺伝子治療の前臨床試験 (2009)
  • Author(s): 濱田雄行、張〓、白川利朗
  • Organizer: 第7回日本臨床腫瘍学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] ポリマーで多重層コーティングしたアデノウィルスの調製とその抗腫瘍効果 (2009)
  • Author(s): 小山義之、芳原智恵子、濱田雄行
  • Organizer: 第19回バイオ・高分子シンポジウム
  • Place of Presentation: 東京
• [Presentation] Intravenous Injection of the carrier-cell based oncolytic adenovirus suppresses the growth of multiple lung tumors in mouse squamous cell carcinoma model. (2009)
  • Author(s): Shirakawa T., Hamada K., Kitajima, S., Mitsuoka, C., Saito A, Matsuoka, T., Adhim, Z., Terao, S., Gotoh, A., Nibu, K., Fujisawa, M., Lee, K.
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 大阪大学、大阪
• [Presentation] ヒト膀胱癌細胞株に対するIAI.3Bプロモーターを組み込んだ制限増殖型アデノウイルスベクターの有用性の検討 (2009)
  • Author(s): 寺尾秀治、Acharya Bishunu、鈴木透、濱田雄行、後藤章暢
  • Organizer: 第59回日本泌尿器科学会中部総会
  • Place of Presentation: 金沢
• [Presentation] 人工エンベロープを付与した腫瘍溶解性アデノウィルスによるガン遺伝子治療効果 (2009)
  • Author(s): 芳原智恵子、小山義之、濱田雄行
  • Organizer: 第31回日本バイオマテリアル学会
  • Place of Presentation: 京都
• [Presentation] ポリアニオンで被覆した微小なプラスミド複合体による抗腫瘍遺伝子治療効果 (2009)
  • Author(s): 小山義之、芳原智恵子、伊藤智子、濱田雄行
  • Organizer: 第31回日本バイオマテリアル学会
  • Place of Presentation: 京都
• [Presentation] Pharmacological and biosafety test of cancer gene therapy by oncolytic ade novirus-infected carrier cell (2009)
  • Author(s): 濱田雄行
  • Organizer: AACR 100th annual meeting 2009
  • Place of Presentation: Denver America
• [Presentation] Preparation of polymer-coated adenovirus particles and their therapeutic effects in mouse tumor models (2009)
  • Author(s): 小山義之, et al
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy
  • Place of Presentation: San Diego America
• [Presentation] Intravenous injection of allogeneic tumor cell vaccine carrying oncolytic adenovirus(AdE3-IAI.3B)suppresses the growth of mMultiple lung tumors in mouse squamous cell carcinoma model (2009)
  • Author(s): Toshiro Shirakawa, et al
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy
  • Place of Presentation: San Diego America
• [Presentation] High level therapeutic effects of fine plasmid/PEI/Hyaluronic acid ternary complex particles in tumor model mouse (2009)
  • Author(s): Tomoko Ito, et al
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy
  • Place of Presentation: San Diego America
• [Presentation] Improved gene transfer into renal carcinoma cells using an adenovirus vector (2009)
  • Author(s): 寺尾秀治, et al
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy
  • Place of Presentation: San Diego America
• [Presentation] Pharmacological and biosafety test of cancer gene therapy by oncolytic adenovirus-infected carrier cell (2009)
  • Author(s): 濱田雄行, et al
  • Organizer: 12th Annual Meeting of American Society of Gene Therapy
  • Place of Presentation: San Diego America
• [Presentation] Polyanion-coated nanoparticle を用いた新たなDDSによる癌特異的免疫遺伝子治療 (2009)
  • Author(s): 濱田雄行, 他
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
• [Presentation] キャリアー細胞を用いたオンコリティックアデノウイルスによる細胞性免疫性癌遣伝子治療の開発 (2009)
  • Author(s): 濱田雄行
  • Organizer: 第68回日本癌学会
  • Place of Presentation: 横浜
• [Presentation] Intravenous Injection of the carrier-cell based oncolytic adenovirus suppresses the growth of multiple lung tumors in mouse squamous cell carcinoma model (2009)
  • Author(s): Toshiro Shirakawa, et al
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 大阪
• [Presentation] 工エンベロープを付与した腫瘍溶解性アデノウィルスによるガン遺伝子治療効果 (2009)
  • Author(s): 芳原智恵子, 他
  • Organizer: 第31回日本バイオマテリアル学会
  • Place of Presentation: 京都
• [Presentation] Pre-clinical studies of GMJ2 1 : Carrie cell-based adenoviral oncolytic virotherapy for head and neck squamous cell carcinoma (2008)
  • Author(s): Toshiro Shirakawa, Katsuyuki Hamada, Shunichi Kitajima, et al
  • Organizer: 2008 International Society for Cell and Gene Therapy of Cancer China Conference
  • Place of Presentation: Shijiazhuang, China
  • Year and Date: 2008-12-19
• [Presentation] 癌完全治癒を目指した癌遺伝子治療薬の研究開発と事業化についての進捗状況 (2008)
  • Author(s): 濱田雄行
  • Organizer: 第6回ジュニアアドベンチャー選手権
  • Place of Presentation: 松山
  • Year and Date: 2008-11-25
• [Presentation] 癌完全治癒を目指した癌遺伝子治療薬の研究開発と事業化 (2008)
  • Author(s): 濱田雄行
  • Organizer: 第6回ジュニアアドベンチャー選手権
  • Place of Presentation: 松山
  • Year and Date: 2008-11-25
• [Presentation] キャリアー細胞による遺伝子治療と動物医薬への応用 (2008)
  • Author(s): 濱田雄行
  • Organizer: 化血研KIKUCHIバイオセミナー「三風会」講演会 講演
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-19
• [Presentation] キャリアー細胞を用いた細胞性免疫性癌遺伝子治療の感染抑制解除機構と前臨床試験 (2008)
  • Author(s): 濱田雄行、川田真美、張〓、白川利朗
  • Organizer: 第46回日本癌治療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] Cellular and immne gene therapy for various solid tumors by IAI.3B promoter-driven oncolytic adenovirus. (2008)
  • Author(s): Hamada K., Kawada, M., Zhang,T.
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] キャリアー細胞を用い細胞性免疫性癌遺伝子治療の感染抑制解除機構と前臨床試験 (2008)
  • Author(s): Katsuyuki Hamada, Mami Kawada, Ting Zhang.
  • Organizer: 第46回日本癌治療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] Cellular and immne gene therapy for various solid tumors by IAI. 3Bpromoter-driven oncolytic adenovirus (2008)
  • Author(s): 濱田雄行, 川田真美, 張?, 白川利朗
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Gene therapy for solid tumors by A549 carrier cells infected with oncolytic adenovirus driven by IAI.3B promoter. (2008)
  • Author(s): Hamada K., Zhang,T., Hattori, Y., Sawazaki, T., Sumida, M.
  • Organizer: 11th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: Boston, USA.
  • Year and Date: 2008-06-01
• [Presentation] Gene therapy for solid tumors by A549 carrier cells infected with oncolytic adenovirus driven by IAI. 3B promoter (2008)
  • Author(s): Katsuyuki Hamada, Ting Zhang, Yuka Hattori, Tetsuya Sawazaki, et al
  • Organizer: 11th Annual Meeting of American Society of Gene Therapy.
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-05-30
• [Presentation] Title : SOCA1 Promoter-driven Replication-selective Adenovims for Oral Cancer Gene Therapy (2008)
  • Author(s): Ting Zhang, Katsuyuki Hanada, Hiroyuki Goda, et al
  • Organizer: 12th International Congress on Oral Cancer
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-05-28
• [Presentation] キャリアー細胞を用いたオンコリティックアデノウイルスによる癌特異的遺伝子治療とその免疫原性克服機構 (2008)
  • Author(s): 濱田雄行
  • Organizer: 九州大学先端高分子治療科講演会 講演
  • Place of Presentation: 福岡
  • Year and Date: 2008-04-28
• [Presentation] キャリアー細胞全身投与による卵巣癌特異的細胞性免疫性遺伝子治療 (2008)
  • Author(s): 濱田雄行
  • Organizer: 第60回日本産科婦人科学会総会・学術講演会
  • Place of Presentation: パシフイコ横浜、横浜市
  • Year and Date: 2008-04-15
• [Presentation] キャリアー細胞による液性免疫解除機構を利用した癌遺伝子治療薬の開発 (2008)
  • Author(s): 濱田雄行
  • Organizer: 第8回バイオビジネスコンペJAPAN
  • Place of Presentation: シティプラザ大阪、大阪市
  • Year and Date: 2008-02-19
• [Presentation] 癌治療フォーラム-癌は不治の病ではない-「癌治療研究の現状と今後の展開」 (2008)
  • Author(s): パネルディスカッション 濱田雄行、高知発
  • Organizer: JSTイノベーションサテライト高知
  • Place of Presentation: 高知
  • Year and Date: 2008-02-10
• [Presentation] Induction of antitumor effect by CTL response after the preimmunization of adenovirus in carrier cell-mediated oncolytic adenovirus infection. (2008)
  • Author(s): Hamada K., Zhang,T., Nagai, A., Sawazaki, T., Sumida, M.
  • Organizer: 99^<th> AACR
  • Place of Presentation: San Diego, USA.
• [Presentation] Title : SCCA1 Promoter-driven Replication-selective Adenovirus for Oral Cancer Gene Therapy. (2008)
  • Author(s): Zhang,T., Hamada K., Goda, H., Nakashiro, K., Hamakawa, H.
  • Organizer: 12th International Congress on Oral Cancer
  • Place of Presentation: Shanghai, China.
• [Presentation] Pre-clinical studies of GMJ2.1 : Carrie cell-based adenoviral oncolytic virotherapy for head and neck squamous cell carcinoma. (2008)
  • Author(s): Shirakawa. T., Hamada K., Kitajima, K., Mitsuoka, C., Jin, H., Morishita, N., Yamaoka, N., Terao S., Gotoh A., Matsuoka, T., Adhim, Z., Nibu, K., Fujisawa, M., Kawabata, M., Wu, J., Zhou, L., Cao, H., Huang, W.
  • Organizer: International Society for Cell and Gene Therapy of Cancer China Conference.
  • Place of Presentation: Shijiazhuang, China.
• [Presentation] 極微細なプラスミド複合体の調製とその治癒効果 (2008)
  • Author(s): 小山義之、濱田雄行、伊藤智子、芳原智恵子
  • Organizer: 第19回アンチセンスRNA-DNA研究会
  • Place of Presentation: 福岡
• [Book] Cancer gene therapy(13 gynecologic gene therapy)(Research signpost, M.Tagawa edt.) (2010)
  • Author(s): 濱田雄行
• [Book] Cancer gene therapy, 13 gynecologic gene therapy (2010)
  • Author(s): Katsuyuki Hamada
  • Publisher: Research signpost, M.Tagawa edt.
• [Book] 平成19年度ジュニアアドベンチャー選手権者による進歩状況報告書(平成20年11月25日、最優秀賞、癌完全治癒を目指した癌遺伝子治療薬の研究開発と事業化)(愛媛県テクノプラザ編集) (2008)
  • Author(s): 濱田雄行
• [Book] 平成19年度ジュニアアドベンチャー選手権者による進歩状況報告書-癌完全治療を目指した癌遺伝子治療薬の研究開発と事業化- (2008)
  • Author(s): 濱田雄行
  • Publisher: 愛媛県テクノプラザ編集
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] 新規なベクター、それを含有する医薬及び癌の治療方法 (2010)
  • Inventor(s): 小山義之、濱田雄行, 他
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Filing Date: 2010-08-31
• [Patent(Industrial Property Rights)] ウイルスの複合体、それを含有する治療薬及び治療方法 (2010)
  • Inventor(s): 小山義之、濱田雄行
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Filing Date: 2010-04-28
• [Patent(Industrial Property Rights)] ウイルスの複合体、それを含有する治療薬及び治療方法 (2010)
  • Inventor(s): 小山義之、濱田雄行
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Filing Date: 2010-04-28
  • Overseas
• [Patent(Industrial Property Rights)] 遺伝子導入治療剤 (2010)
  • Inventor(s): 濱田雄行, 他
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Acquisition Date: 2010-05-27
• [Patent(Industrial Property Rights)] 遺伝子導入治療剤 (2010)
  • Inventor(s): 濱田雄行, 他
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Acquisition Date: 2010-05-27
  • Overseas
• [Patent(Industrial Property Rights)] 遺伝治療剤 (2010)
  • Inventor(s): 濱田雄行, 他
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Acquisition Date: 2010-08-12
• [Patent(Industrial Property Rights)] 遺伝治療剤 (2010)
  • Inventor(s): 濱田雄行, 他
  • Industrial Property Rights Holder: アルファ・ナノメディカ株式会社
  • Acquisition Date: 2010-08-12
  • Overseas