Visualizing and dynamic analysis of spiral ganglion cell progenitors b using of human hearing-loss model animals

• Project/Area Number: 20591980
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University
• Principal Investigator: KOJIMA Ken 京都大学, 医学研究科, 客員研究員 (60378685)
• Co-Investigator(Kenkyū-buntansha): OUTSUKA Toshiyuki 京都大学, ウイルス研究所, 准教授 (20324709) ; IKEDA Katsuhisa 順天堂大学, 医学部, 教授 (70159614)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: らせん神経節細胞 / 前駆細胞 / 衛星細胞 / lineage tracing / 難聴モデル動物 / in vivo / in vitro / 難聴 / 難聴モデルマウス / 組織学的検索 / トランスジェニック動物 / ラセン神経節 / 動態 / ヒト難聴等価モデル / 発達 / 音響障害 / 聴覚再生

Research Abstract

Stem cell marker protein expressing cells identified by use of transgenic animals were analyzed in vivo and in vitro. In spite of different lineage from spiral ganglion neurons, satellite cells expressing stem cell markers in spiral ganglion were induced to neural phenotypes in vitro. In vivo analyses showed that no satellite cell were induced to neural phenotypes. These results indicated that some kinds of controls of proliferation and differentiation of the satellite cells were needed for regeneration of spiral ganglion neurons in vivo.

Research Products

• [Journal Article] 人工内耳装用学童児の聞き取り能力における教室音環境の影響 (2012)
  • Author(s): 森尚彫、伊藤壽一、平海晴一、山口忍、柴田尚美、松井理直、山本典夫、坂本達則、岩井詔子、小島憲、松本昌宏、扇田秀章
  • Journal Title: Audiology Japan Volume: 55(2) Pages: 138-145
• [Journal Article] Mechanical stress-induced reactive gliosis in the auditory nerve and cochlear nucleus (2011)
  • Author(s): Sekiya T, Matsumoto M, Kojima K, Ono K., Kikkawa YS, Kada S, Ogita H, Horie RT, Viola A, Holley MC, Ito J
  • Journal Title: J Neurosurg Volume: 114 Pages: 414-25
  • Peer Reviewed
• [Journal Article] Mechanical stress-induced reactive gliosis in the auditory nerve and cochlear nucleus (2010)
  • Author(s): Sekiya T, Matsumoto M, Kojima K, Ono K, Kikkawa YS, Kada S, Ogita H, Horie RT, ViolaA, Holley MC, Ito J
  • Journal Title: J Neurosurg Volume: 114(2) Pages: 414-25
• [Journal Article] Mechanical stress-induced reactive gliosis in the auditory nerve and cochlear nucleus. (2010)
  • Author(s): Sekiya T, Matsumoto M, Kojima K, Ono K, Kikkawa YS, Kada S, Ogita H, Horie RT, Viola A, Holley MC, Ito J
  • Journal Title: J Neurosurg. E-pub(E-pub)(in press)
  • Peer Reviewed
• [Journal Article] Silencing p27 reverses post-mitotic state of supporting cells in neonatal mouse cochleae (2009)
  • Author(s): Ono K, Nakagawa T, Kojima K, Matsumoto M, Kawauchi T, Hoshino M, Ito J
  • Journal Title: Mol Cell Neurosci Volume: 42(4) Pages: 391-8
  • NAID: 120001700386
• [Journal Article] Selective vulnerability of adult cochlear nucleus neurons to de-afferentation by mechanical compression (2009)
  • Author(s): Sekiya T, Canlon B, Viberg A, Matsumoto M, Kojima K, Ono K, Yoshida A, Kikkawa YS, Nakagawa T, Ito J
  • Journal Title: Exp Neurol Volume: 218(1) Pages: 117-23
• [Journal Article] Silencing p27 reverses post-mitotic state of supporting cells in neonatal mouse cochleae. (2009)
  • Author(s): Ono K, Nakagawa T, Kojima K, Matsumoto M, Kawauchi T, Hoshino M, Ito J.
  • Journal Title: Mol Cell Neurosci. 42(4) Pages: 391-398
  • NAID: 120001700386
  • Peer Reviewed
• [Journal Article] Selective vulnerability of adult cochlear nucleus neurons to de-afferentation by mechanical compression. (2009)
  • Author(s): Sekiya T, Canlon B, Viberg A, Matsumoto M, Kojima K, Ono K, Yoshida A, Kikkawa YS, Nakagawa T, Ito J.
  • Journal Title: Exp Neurol. 218(1) Pages: 117-123
  • Peer Reviewed
• [Journal Article] Selective vulnerability of adult cochlear nucleus neurons to de-afferentation by mechanical compression. (2009)
  • Author(s): Sekiya T, Canlon B, Viberg A, Matsumoto M, Kojima K, Ono K, Yoshida A, Kikkawa YS, Nakagawa T, Ito J.
  • Journal Title: Exp Neurol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells (2008)
  • Author(s): Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J
  • Journal Title: J Neurosci Res Volume: 86(14) Pages: 3075-85
• [Journal Article] An animal experimental model of auditory neuropathy induced in rats by auditory nerve compression. (2008)
  • Author(s): Matsumoto M, Sekiya T, Kojima K, Ito J.
  • Journal Title: Exp Neurol 210 Pages: 248-56
  • Peer Reviewed
• [Journal Article] "Replacement of diseased auditory neurons by cell transplantation". (2008)
  • Author(s): Sekiya T, Kojima K, Matsumoto M, Ito J
  • Journal Title: Front Biosci. 13 Pages: 2165-76
  • Peer Reviewed
• [Presentation] 当院で経験した骨固定式骨導補聴器Bahaシステムの問題点 (2011)
  • Author(s): 小島憲、松本昌宏、扇田秀章、森尚彫、大西晶子
  • Organizer: 平成23年度逓信医学年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-10-22
• [Presentation] 当院における人工内耳と補聴器の両耳装用症例の補聴器併用効果について (2011)
  • Author(s): 松本昌宏、小島憲、扇田秀章、森尚彫、大西晶子
  • Organizer: 平成23年度逓信医学年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-10-22
• [Presentation] 当院における人工内耳と補聴器の両耳装用症例の検討 (2011)
  • Author(s): 松本昌宏、小島憲、扇田秀章、大西晶子、森尚彫、伊藤壽一
  • Organizer: 第112回日本耳鼻咽喉科学会総会学術講演会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-05-19
• [Presentation] 当院における片側聾患者に対するBahaRシステムBP100の使用経験 (2011)
  • Author(s): 小島憲、松本昌宏、扇田秀章、森尚彫、大西晶子、伊藤壽一
  • Organizer: 第112回日本耳鼻咽喉科学会総会
  • Place of Presentation: 京都
• [Presentation] 当院における人工内耳と補聴器の両耳装用症例の検討 (2011)
  • Author(s): 松本昌宏、小島憲、扇田秀章、森尚彫、大西晶子、伊藤壽一
  • Organizer: 第112回日本耳鼻咽喉科学会総会
  • Place of Presentation: 京都
• [Presentation] ラセン神経節衛星細胞のin vitroにおける自己複製能に関する解析 (2008)
  • Author(s): 小島憲、小野和也、伊藤壽一
  • Organizer: 第18回日本耳科学会総会・学術講演会
  • Place of Presentation: 神戸
  • Year and Date: 2008-10-16
• [Presentation] Satellite cells include stem cells in thespiral ganglion of the postnatal mouseinner ear (2008)
  • Author(s): K. Kojima, T. Ohtsuka, T, Tateya, K, Ono, S. Takebayashi, R, Kageyama
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] Satellite cells include stem cells in the spiral ganglion of the postnatal mouse inner ear (2008)
  • Author(s): K. Kojima, T. Ohtsuka, T, Tateya, K, Ono, S. Takebayashi, R, Kageyama
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] ラセン神経節衛星細胞の多分化能についての検討 (2008)
  • Author(s): 小島憲、小野和也、伊藤壽一
  • Organizer: 第109回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-16