| Project/Area Number |
20591982
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Osaka University |
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Principal Investigator |
DOI Katsumi Osaka University, 医学部, 教授 (40243224)
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| Co-Investigator(Kenkyū-buntansha) |
KITAHARA Tadashi 大阪大学, 医学系研究科, 講師 (30343255)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | メニエール病 / ヒト内リンパ嚢 / DNAマイクロアレイ / 聴神経腫瘍 / 遺伝子発現 |
|---|
| Research Abstract |
To identify genesrelated to the pathogenesis of Meniere's disease (MD), gene expression profilings of the human endolymphatic sac (ES) harvested from the cases of both MD and Vestibular Schwannoma (VS) were determined by DNA microarray analysis. In the present study, we could successfully perform the DNA microarray analysis of the human ES. In spite of a tiny amount of total RNA obtained from this specialized tissue, approximately 7500 genes were identified to be there. In comparison of gene expression profiling between the human ES of both MD and VS cases, some genes were significantly up-regulated and others were down-regulated in the human ES of MD cases. The DNA microarray analysis in the present study may provide a large-scale understanding of gene expression profiling in the human ES and make it possible in future to identified the genes crossly related to the pathogenesis of MD.
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