Gene Expression Profiling of Human Endolymphatic Sac by DNA Microarray Analysis
| Project/Area Number |
20591982
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Osaka University |
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Principal Investigator |
DOI Katsumi Osaka University, 医学部, 教授 (40243224)
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| Co-Investigator(Kenkyū-buntansha) |
KITAHARA Tadashi 大阪大学, 医学系研究科, 講師 (30343255)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | メニエール病 / ヒト内リンパ嚢 / DNAマイクロアレイ / 聴神経腫瘍 / 遺伝子発現 |
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| Research Abstract |
To identify genesrelated to the pathogenesis of Meniere's disease (MD), gene expression profilings of the human endolymphatic sac (ES) harvested from the cases of both MD and Vestibular Schwannoma (VS) were determined by DNA microarray analysis. In the present study, we could successfully perform the DNA microarray analysis of the human ES. In spite of a tiny amount of total RNA obtained from this specialized tissue, approximately 7500 genes were identified to be there. In comparison of gene expression profiling between the human ES of both MD and VS cases, some genes were significantly up-regulated and others were down-regulated in the human ES of MD cases. The DNA microarray analysis in the present study may provide a large-scale understanding of gene expression profiling in the human ES and make it possible in future to identified the genes crossly related to the pathogenesis of MD.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Clinical characteristics of delayed endolymphatic hydrops in Japan : A nationwide survey by the Peripheral Vestibular Disorder Research Committee of Japan.2010
Author(s)
H.Shojaku, Y.Watanabe, N.Takeda, T.Ikezono, M.Takahashi, A.Kakigi, J.Ito, K.Doi, M.Suzuki, M.Takumida, K.Takahashi, H.Yamashita, I.Koizuka, S.Usami, M.Aoki, H.Naganuma
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Journal Title
Acta Otolaryngol 130
Pages: 1135-40
Related Report
Peer Reviewed
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[Presentation] 難治性BPPV患者の責任半規管の同定と半規管遮断術2009
Author(s)
吉波和隆,北原糺,今井貴夫,大崎康宏,増村千佐子,木澤薫,岡崎鈴代,前川千絵,土井勝美,猪原秀典,堀井新
Organizer
第68回日本めまい平衡医学会
Place of Presentation
徳島
Year and Date
2009-11-27
Related Report
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[Presentation] 内リンパ嚢手術後および半規管遮断術後に生じる低周波数領域の気骨導差2009
Author(s)
北原糺,吉波和隆,川島貴之,今井貴夫,大崎康宏,木澤薫,増村千佐子,岡崎鈴代,前川千絵,土井勝美,猪原秀典,堀井新
Organizer
第68回日本めまい平衡医学会
Place of Presentation
徳島
Year and Date
2009-11-27
Related Report
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[Presentation] 内リンパ嚢開放術2009
Author(s)
土井勝美
Organizer
第68回日本めまい平衡医学会
Place of Presentation
ホテル・クレメント (徳島県)
Year and Date
2009-11-27
Related Report
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