The role of water channel of aquaporins and water pump of Na-K-2Cl co-transporter on the water homeostasis of the inner ear.

• Project/Area Number: 20591987
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kochi University
• Principal Investigator: TAKEDA Taizo (2008, 2010) Kochi University, 名誉教授 (50115763); 竹田 泰三 (2009) Kochi University, 名誉教授 (50115705)
• Co-Investigator(Kenkyū-buntansha): NISHIOKA Rie 高知大学, 教育研究部・医療学系, 助教 (20398051) ; KAKIGI Akinobu 東京大学, 医学部, 講師 (60243820)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: aquaporin / イオン共輸送体 / 血管条 / 内リンパ嚢 / vasopressin / 水チャネル / NKCC1 / NKCC2 / stria vascularis / endolymphatic sac / bumetanide / 内耳水代謝

Research Abstract

The expressions of many subtypes of aquaporins (AQP) including AQP-2, vasopressin type 2 receptor, and NKCC1 were molecular biologically and immunohistochemically evaluated. Since the application of vasopressin, bumetanide, and OPC-31260 exerted the morphological influence on the intrastrial space, AQP2-vasopressin mediated water transport and water flow through the Na-K-Cl co-transporter might work actively in the inner ear fluid homeostasis.

Research Products

• [Journal Article] Hormonal Aspects of Meniere's Disease on the Basis of Clinical and Experimental Studies. (2010)
  • Author(s): Taizo Takeda, Setsuko Takeda, Akinobu Kakigi, Rie Nishioka, Daizo Taguchi, Masahiko Nishimura, Hiroaki Nakatani.
  • Journal Title: ORL J Otorhinolaryngol Relat Spec 71(Suppl 1) Pages: 1-9
• [Journal Article] (2010)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Okada T, Doi K.
  • Journal Title: ORL J Otorhinolaryngol Relat Spec. 71(Suppl 1) Pages: 10-15
• [Journal Article] Expression of Aquaporins and Vasopressin type 2 receptor in the stria vascularis of the cochlea. (2010)
  • Author(s): Nishioka R, Takeda T, Kakigi A, Okada T, Takebayashi S, Nishimura M, Taguchi D, Hyodo M.
  • Journal Title: Hear Res. 260 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Hormonal Aspects of Meniere's Disease on the Basis of Clinical and Experimental Studies (2010)
  • Author(s): Taizo Takeda, Setsuko Takeda, Akinobu Kakigi, Rie Nishioka, Daizo Taguchi, Masahiko Nishimura, Hiroaki Nakatani
  • Journal Title: ORL J Otorhinolaryngol Relat Spec Volume: 71 Suppl 1 Pages: 1-9
• [Journal Article] Bumetanide-Induced Enlargement of the Rat Intrastrial Space and Effects of a Vasopressin Type 2 Antagonist (2010)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Okada T, Doi K
  • Journal Title: ORL J Otorhinolaryngol Relat Spec Volume: 71 Suppl 1 Pages: 10-15
• [Journal Article] Expression of Aquaporins and Vasopressin type 2 receptor in the stria vascularis of the cochlea (2010)
  • Author(s): Nishioka R, Takeda T, Kakigi A, Okada T, Takebayashi S, Nishimura M, Taguchi D, Hyodo M
  • Journal Title: Hear Res Volume: 260 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Expression of Aquaporin1, 3, and 4, NKCC1, and NKCC2 in the Human Endolymphatic Sac. (2009)
  • Author(s): Kakigi A, Nishimura M, Takeda T, Taguchi D, Nishimura R.
  • Journal Title: Auris Nasus Larynx. 36 Pages: 135-139
  • Peer Reviewed
• [Journal Article] Time course changes of vasopressin-induced enlargement of the rat intrastrial space and the effects of a vasopressin type 2 antagonist. (2009)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Okada T, Doi K.
  • Journal Title: Acta Otolaryngol 129 Pages: 709-715
  • Peer Reviewed
• [Journal Article] Expression of Aquaporin1, 3, and 4, NKCC1, and NKCC2 in the Human Endolymphatic Sac (2009)
  • Author(s): Kakigi A, Nishimura M, Takeda T, Taguchi D, Nishimura R
  • Journal Title: Auris Nasus Larynx 36 Pages: 135-139
  • Peer Reviewed
• [Journal Article] Time course changes of vasopressin-induced enlargement of the rat intrastrial space amd the effects of a vasopressin type 2 antagonist (2009)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Okada T, Doi K
  • Journal Title: Acta Otolaryngol 129 Pages: 709-715
  • Peer Reviewed
• [Journal Article] Expression and immunelocalization of Aquaporin-6 (Aqp6) in the rat inner ear. (2008)
  • Author(s): Taguchi D, Takeda T, Kakigi A, Okada T, Nishioka R, Kitano H.
  • Journal Title: Acta Otolaryngol 128 Pages: 832-840
  • Peer Reviewed
• [Journal Article] Expression and immunolocalization of Aquaporin-6 (Aqp6) in the rat inner ear (2008)
  • Author(s): Taguchi D, Takeda T, Kakigi A, Okada T, Nishioka R, Kitano H
  • Journal Title: Acta Otolaryngol 128 Pages: 832-840
  • Peer Reviewed
• [Journal Article] Expression of aquaporins, vasopressin type 2receptor and Na+-K+-Cl- cotransporters in the rat endolymphatic sac
  • Author(s): Nishimura M, Kakigi A, Takeda T, Takeda S, Doi K
  • Journal Title: Acta Otolaryngol (印刷中)
  • Peer Reviewed
• [Presentation] Aquaporins as Potentials Drug Targets for Meniere's Disease and Its Related Diseases (2010)
  • Author(s): Takeda T, Kakigi A
  • Organizer: 6th International Symposium on Meniere's disease and Inner Ear Disorders
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-11-16
• [Presentation] Expression of aquaporin1, 2, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac. (2008)
  • Author(s): Kakigi A, Nishimura M, Takeda T, Taguchi D, Nishioka R.
  • Organizer: 45^<th> Inner Ear Biology Workshop
  • Place of Presentation: Ferrara, Italy.
  • Year and Date: 2008-09-23
• [Presentation] Expression of aquaporin1, 2, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac (2008)
  • Author(s): Kakigi A, Nishimura M, Takeda T, Taguchi D, Nishioka R
  • Organizer: 45^<th> Inner Ear Biology Workshop
  • Place of Presentation: Ferrara, Italy
  • Year and Date: 2008-09-23
• [Presentation] Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl cotransporters in the rat endolymphatic sac. (2008)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Takeda S, Nishioka R, Doi K.
  • Organizer: 45th Inner Ear Biology Workshop.
  • Place of Presentation: Ferrara, Italy.
  • Year and Date: 2008-09-22
• [Presentation] Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl cotransporters in the rat endolymphatic sac (2008)
  • Author(s): Nishimura M, Kakigi A, Takeda T, Takeda S, Nishioka R, Doi K
  • Organizer: 45th Inner Ear Biology Workshop.
  • Place of Presentation: Ferrara, Italy
  • Year and Date: 2008-09-22
• [Book] Handbook of Experimental Pharmacology 190, Aquaporins (ed. by Beit E). Aquaporins as potential drug targets for Meniere's disease and its related diseases (2009)
  • Author(s): Takeda T, et al.
  • Publisher: Springer
• [Book] Handbook of Experimental Pharmacology 190, Aquaporins (ed. by Beit E). Aquaporins as potential drug targets for Meniere's disease and its related diseases (2009)
  • Author(s): Takeda T, Taguchi D
  • Publisher: Springer