Cochlear hair cell regeneration by re-activation of cell cycle of the supporting cells

• Project/Area Number: 20591989
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kumamoto University
• Principal Investigator: MINODA Ryosei Kumamoto University, 大学院・生命科学研究部, 准教授 (30284772)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 再生 / 内耳 / 再生医学 / 支持細胞

Research Abstract

P27^<kip1> has a function to suppress the proliferation of supporting cell in the adult mammal cochlea. Considering this, downregulation of P27^<kip1> in adult mammal cochlea may cause proliferation of the supporting cells.
P27^<kip1> existed in supporting cells in normal mouse organ of Corti. P27^<kip1> downregulation utilizing siRNA for P27^<kip1> resulted in re-entry into cell cycle of the supporting cells and proliferation of the supporting cells. Nevertheless, those supporting cells did not show phenotype of cochlear hair cells.

Research Products

• [Journal Article] External auditory canal stenting utilizing a useful rolled,tapered silastic sheet (RTSS)post middle ear surgery (2010)
  • Author(s): Minoda R、Haruno T, Miwa T, Kumai Y, Sanuki T, Yumoto E.
  • Journal Title: AURIS NASUS LARYNX 37(6) Pages: 680-684
  • Peer Reviewed
• [Journal Article] PC3 is involved in the shift from proliferation to differentiation and maturation in spiral ganglion neurons. (2010)
  • Author(s): Hayashida M, Minoda R, Shinmyo Y, Ohta K, Tanaka H, Yumoto E.
  • Journal Title: Neuroreport 21 Pages: 90-93
  • Peer Reviewed
• [Journal Article] PC3 is involved in the shift from proliferation to differentiation and maturation in spiral ganglion neurons. (2010)
  • Author(s): Neuroreport
  • Journal Title: Hayashida M, Minoda R, Shinmyo Y, Ohta K, Tanaka H, Yumoto E. Volume: 27 Pages: 90-93
  • Peer Reviewed
• [Journal Article] Aeration of tympanum for hearing rehabilitation in staged tympanoplasty with mastoid cavity (2009)
  • Author(s): Komori M, Yanagihara N, Hinohira Y, Minoda R.
  • Journal Title: Surgery of the Ear Pages: 278-279
  • Peer Reviewed
• [Presentation] Tsukushi Gene Expression in the Mouse Cochleae (2010)
  • Author(s): Minoda R、Hayashida M, Miwa T, Yumoto E.
  • Organizer: 6th International Symposium on Meniere's Disease and Inner Ear Disorders
  • Place of Presentation: 京都(京都国際会館)
• [Presentation] Hospital Anxiety and Depression Scale(HADS)Test for Balance Disorder Patients (2010)
  • Author(s): Miwa T、Minoda R, Yumoto E.
  • Organizer: 6th International Symposiumon Meniere's Disease and Inner Ear Disorders
  • Place of Presentation: 京都(京都国際会館)
• [Presentation] 反対側耳への人工内耳再埋め込み手術を余儀なくされたBruton型低γグロブリン血症の1症例 (2010)
  • Author(s): 蓑田涼生、増田聖子、三輪徹、湯本英二、高橋晴雄
  • Organizer: 第20回日本耳科学会総会・学術講演会
  • Place of Presentation: 愛媛(ひめぎんホール)
• [Presentation] マウス蝸牛におけるTsukushiの発現について (2010)
  • Author(s): 林田桃子、蓑田涼生、湯本英二
  • Organizer: 第20回日本耳科学会総会・学術講演会
  • Place of Presentation: 愛媛(ひめぎんホール)
• [Presentation] ポリアルギニンによる胎生期内耳(耳胞)への蛋白質導入法 (2010)
  • Author(s): 三輪徹、蓑田涼生、林田桃子、湯本英二
  • Organizer: 第20回日本耳科学会総会・学術講演会
  • Place of Presentation: 愛媛(ひめぎんホール)
• [Presentation] Mastoid Cavity Obliteration Using β-Tricalcium Phosphate after Mastoidectomy (2009)
  • Author(s): 蓑田涼生、湯本英二
  • Organizer: 第10回台日耳鼻咽喉科学会議
  • Place of Presentation: 台湾(HotelRoyal Chiao Hsi)
• [Presentation] シリコン板を用いたMastoid Cortex Plastyの試み (2009)
  • Author(s): 蓑田涼生、春野尊、湯本英二
  • Organizer: 第19回日本耳科学会
  • Place of Presentation: 東京(京王プラザホテル)
• [Presentation] PC3 is involved in the switch from proliferation to differentiation in the spiral ganglion cells of the cochlea (2009)
  • Author(s): 林田桃子、蓑田涼生、湯本英二
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: エジンバラ(Edinburgh International Conference Centre)
• [Presentation] Mastoidectomy reconstruction technique (2008)
  • Author(s): 蓑田涼生
  • Organizer: 8th International Conference on Cholesteatoma & Ear Surgery
  • Place of Presentation: トルコ(Kervansaray Lala Hotel)
  • Year and Date: 2008-06-18
• [Presentation] 遺伝子治療による蝸牛有毛細胞再生の可能性 (2008)
  • Author(s): 蓑田涼生
  • Organizer: 第23回九州連合地方部会学術講演会
  • Place of Presentation: 沖縄(ロジワール沖縄)
  • Year and Date: 2008-05-31