Molecular pathogenesis of middle ear mucosal proliferation during bacterial otitis media

• Project/Area Number: 20591992
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Juntendo University
• Principal Investigator: FURUKAWA Masayuki Juntendo University, 医学部, 准教授 (20359524)
• Co-Investigator(Kenkyū-buntansha): IKEDA Katuhisa 順天堂大学, 医学部, 教授 (70159614) ; KUSUNOKI Takeshi 順天堂大学, 医学部, 准教授 (30248025) ; YOKOI Hidenori 順天堂大学, 医学部, 准教授 (80317487)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: DNA microarray / 中耳粘膜肥厚 / 中耳炎 / Haemophilus influenzae / 中耳粘膜 / Haemophilus influenza / DNA array / 中耳炎モデル動物

Research Abstract

We used gene array to assess gene expression in the mouse middle ear in the first 24h following exposure to Haemophilus influenzae. We observed 513 transcripts that were significantly up-regulated 3h after inoculation, while only 2 transcripts were down-regulated. At 6h, 658 up-regulated transcripts were noted, as compared to 188 down-regulated genes. By 24h after bacterial exposure, 1350 transcripts showed increased expression while 1632 exhibited reduced expression.

Research Products

• [Journal Article] Expression of IL-17 and its role in bone destruction in human middle ear cholesteatoma. (2010)
  • Author(s): Furukawa M, Kusunoki T, Onoda J, Ikeda K., Haruyama T
  • Journal Title: ORL J Otorhinolaryngol Relat Spec. 72(6) Pages: 325-331
  • Peer Reviewed
• [Journal Article] A randomized prospective study of oral levofloxacin vs intravenous flomoxef prophylaxis in postoperative infection after endoscopic sinus surgery. (2010)
  • Author(s): Inosita A, Yokoi H, Matsumoto F, Yao T, Kawano K Furukawa M, Ikeda K
  • Journal Title: Am J Otolaryngol. 31(5) Pages: 360-363
  • Peer Reviewed
• [Journal Article] Overexpression of GLUT-1 in the invasion front is associated with depth of oral squamous cell carcinoma and prognosis. (2010)
  • Author(s): Ohba S, Fujii H, Ito S, Fujimaki, M, Matsumoto F, Furukawa M, Yokoyama J, Kusunoki T, Ikeda K, Hino O
  • Journal Title: J Oral Pathol Med. 39(1) Pages: 74-78
  • Peer Reviewed
• [Journal Article] Development of distortion product otoacoustic emissions in C57BL/6J mice. (2009)
  • Author(s): Narui Y, Minekawa A, Iizuka T, Furukawa M, Kusunoki T, Koike T, Ikeda K
  • Journal Title: Int J Audiol. 48(8) Pages: 576-581
  • Peer Reviewed
• [Journal Article] Prevention of ultrasonic coagulator-mediated mucoperiosteal flap injury and defects by using a clip manipulation in the resection of the posterior nasal nerve. (2009)
  • Author(s): Ikeda K, Yokoi H, Saito T, kawano K, Yao T, Furukawa M, Kusunoki T
  • Journal Title: Rhinology. 47(1) Pages: 45-47
  • Peer Reviewed
• [Journal Article] Eotaxin-1, -2, and-3 immunoreactivity and protein concentration in the nasal polyps of eosinophilic chronic rhinosinusitis patients. (2009)
  • Author(s): Yao T, Kojima Y, Koyanagi A, Yokoi H, Saito T, Kawano K, Furukawa M, Kusunoki T, Ikeda K
  • Journal Title: Laryngoscope. 119(6) Pages: 1053-1059
  • Peer Reviewed
• [Journal Article] Laryngeal stenosis in epidermolysis bullosa dystrophica. (2009)
  • Author(s): Haruyama T, Furukawa M, Matsumoto F, Kawano K, Ikeda K
  • Journal Title: Auris Nasus Larynx. 36(1) Pages: 106-109
  • Peer Reviewed
• [Journal Article] Clinical effectiveness of ototopical application of mupirocin ointment in methicillin-resistant Staphylococcus aureus otorrhea. (2008)
  • Author(s): Furukawa M, Minekawa A, Haruyama T, Narui Y, Sugita G, Sugita R, Kusunoki T, Ikeda K
  • Journal Title: Otol Neurotol. 29(5) Pages: 676-678
  • Peer Reviewed
• [Journal Article] Postnasal development of the organ of Corti in dominant-negative Gjb2 transgenic mice. (2008)
  • Author(s): Inoshita A, Iizuka T, Okamura HO, Minekawa A, Kojima K, Furukawa M, Kusunoki T, Ikeda K
  • Journal Title: Neuroscience. 156(4) Pages: 1039-1047
  • Peer Reviewed
• [Presentation] Surgical management of myringosclerosis over the whole tympanic membrane in simple underlay myringoplasty (2009)
  • Author(s): Masayuki Furukawa, et al
  • Organizer: 6^<th> extraordinary international symposium on recent advances in Otitis Media
  • Place of Presentation: Seoul
  • Year and Date: 2009-05-07
• [Remarks] ホームページ等