Identification of regenerating factors that induce regeneration of spiral ligament

• Project/Area Number: 20591996
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Okayama University
• Principal Investigator: SON Koui Okayama University, 大学院・医歯薬学総合研究科, 外国人客員研究員 (40425773)
• Co-Investigator(Kenkyū-buntansha): MATSUNAGA Tatsuo 独立行政法人国立病院機構東京医療センター(臨床研究センター), 聴覚平衡研究部, 室長 (90245580)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 骨髄間葉系幹細胞 / 内耳再生 / 蝸牛外側壁線維細胞 / 再生医学 / 成長因子 / TGF / TGFβ

Research Abstract

Spiral ligament fibrocytes (SLFs) play important role in normal hearing as well as in several types of sensorineural hearing loss attributable to inner ear homeostasis disorders. In this study we identified TGFβsecreted from mesenchymal stem cells (MSCs) had a stimulatory effect on proliferation of SLFs and the effect was blocked by adding TGFβ inhibitor. Our results suggested TGFβsecreted from MSCs promote the proliferation of SLFs by interaction with other factors.

Research Products

• [Journal Article] Acoustic overstimulation-induced apoptosis in fibrocytes of the cochlea spiral limbus of mice. (2011)
  • Author(s): Cui Y, Sun GW, Yamashita D, Kanzaki S, Matsunaga T, Fujii M, Kaga K, Ogawa K.
  • Journal Title: Eur Arch Otorhinolaryngol. Volume 268, Number 7 Pages: 973-978
  • Peer Reviewed
• [Journal Article] Acoustic overstimulation-induced apoptosis in fibrocytes of the cochlear spiral limbus of mice. (2011)
  • Author(s): Cui Y, Sun GW, Yamashita D, Kanzaki S, Matsunaga T, Fujii M, Kaga K, Ogawa K
  • Journal Title: Eur Arch Otorhinolaryngol Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 松永達雄、孫廣?、務台英樹
  • Journal Title: 小耳症・外耳道閉鎖に対する機能と形態の再建(金原出版) Pages: 11-16
• [Presentation] Functional interaction between Mesenchymal stem cells (MSC) and spiral ligament fibrocytes underlies hearing recovery after MSC transplantation into the inner ear. (2009)
  • Author(s): Guangwei Sun, Masato Fujii, Tetsuo Matsunaga
  • Organizer: Cell Transplant Society (CTS)
  • Place of Presentation: Okayama
• [Presentation] 内耳におけるシグマ1受容体の生後は発達に伴う発現バターン (2008)
  • Author(s): 孫廣〓、山下大介、松長達雄、藤井正人
  • Organizer: 第18回日本耳科学総会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 音響外傷性難聴に対するSA4503の内耳神経保護効果 (2008)
  • Author(s): 山下大介、孫廣〓、神埼晶、松永達雄、小川郁
  • Organizer: 第18回日本耳科学会総会 学術講演会
  • Place of Presentation: 神戸
• [Presentation] 急性内耳エネルギー不全による蝸牛外側壁線維細胞の可塑的変化 (2008)
  • Author(s): 瀧口洋一郎、孫廣〓、崔勇、務台英樹、幸池浩子、水足邦雄、藤井正人、小川郁、松永達雄
  • Organizer: 第18回日本耳科学会総会学術講演会
  • Place of Presentation: 神戸
• [Presentation] 内耳におけるシグマ1受容体の生後発達に伴う発現パターン (2008)
  • Author(s): 孫 コウイ, 山下大介, 松永達雄, 藤井正人
  • Organizer: 第18日本耳科学会総会学術講演会
  • Place of Presentation: 神戸
• [Book] 小耳症・外耳道閉鎖に対する機能と形態の再建(病因と遺伝子) (2009)
  • Author(s): 松永達雄、孫廣〓、務台英樹
  • Publisher: 金原出版