Dynamic analysis of physiological barrier function of nasal mucosal epithelia
| Project/Area Number |
20592003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
MIWA Masato Dokkyo Medical University, 耳鼻咽喉科, 准教授 (80247650)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Shintaro 獨協医科大学, 医学部, 助教 (70337400)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 気道上皮 / バリア機能 / シアストレス / ドラッグデリバリーシステム(DDS) / イオンチャネル / バリア / リポポリサッカライド / ポリカチオン / ポリアニオン / poly(I:C) / ヘパリン / 上皮バリア機能 / 酸化ストレス / アレルギー性鼻炎 / 局所治療 / 抗酸化剤 / 気道粘膜 / 酸ストレス / ドラッグデリバリーシステム |
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| Research Abstract |
The regulation of barrier function of nasal epithelium seems to be involved in the pathogenesis of allergic rhinitis. The measurement of transepidermal water loss has been proved to be an important non-invasive method for assessing the efficiency of the skin as a protective barrier. Although the nasal mucosal epithelium has such protective function as well as skin, the precise mechanism is still obscure. We examined the human nasal transepithelial water loss (TEWL) using our original adapter as well as the measurement of electrical potential differences (PD) of nasal mucosa. We observed that TEWL of the nose was decreased after topical application of hydrogen water rather than ascorbic acid.
We could also examine PD of the posterior wall of the human pharyngeal mucosa. After application of HCl, PD was decreased compared with the basal state. After application of ZnCl2, PD was significantly increased compared with basal state.
We could develop a new model to evaluate the effect of sheer s
… More
tress on the electrical barrier function of nasal mucosal epithelium. We have shown in this study that our original model might be very useful for analyzing the effect of shear stress on the airway epithelium. The data obtained from this model would contribute to understand the pathogenesis and therapy of the upper airway diseases with the rupture of the barrier function by pathological shear stresses.
We have evaluated the short term effect of polycations and polyanions on the transepithelial electrical resistance (TER) of air-interfaced cultured nasal and tracheal epithelial cells excised from guinea pig and to determine the route (transcellualr or paracellular ) that cause the alteration of TER. The reduction in TER of the primary cultured nasal epithelial cells by polycations might be caused via the transcellualr route including ion channels as well as paracellular route like tight junction. Moreover, polyanion like heparin might recover the barrier function of airway mucosal epithelium after application of LPS and poly (I:C). Less
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Report
(4 results)
Research Products
(79 results)
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[Journal Article] Control of transepithelial electrical resistance on primary cultured airway tracheal cells excised from guinea pig2009
Author(s)
Miwa M, Ohmori K, Fukuda K, Kohyama K, Kanoh N, Iwasaki Y, Nakajima N, Yamaguchi S, Ichiwata T, Nagao K, Miwa M, Watanabe K
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Journal Title
Proceeding of Airway Secretion Research 11
Pages: 1-4
Related Report
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[Journal Article] 初代培養気管上皮細胞の上皮膜抵抗に対する酸およびプロトンポンプインヒビターの効果2009
Author(s)
三輪正人, 狩野信和, 岩崎洋子, 中島規幸, 山口晋太郎, 広瀬壮, 阿部実恵子, 三輪真由美, 渡辺建介, 高山賢哉, 一和多俊男, 長尾光修
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Journal Title
日本気管食道科学会会報 60(3)
Pages: 276-280
NAID
Related Report
Peer Reviewed
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[Presentation] 気道上皮のcharge barrierに対するLPSおよびpoly (l : C)の効果2010
Author(s)
三輪正人, 呉松晃, 小松陽, 蓮琢也, 廣瀬壮, 中島規幸, 山口晋太郎, 村上敦史, 狩野信和, 池田洋子, 阿部実恵子, 三輪真由美, 渡辺建介
Organizer
第60回日本アレルギー学会秋季学術大会
Place of Presentation
東京
Related Report
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[Presentation] 抗酸化剤点鼻が抗原鼻誘発反応に与える影響2010
Author(s)
中島規幸, 三輪正人, 廣瀬壮, 狩野信和, 呉松晃, 蓮琢也, 山口晋太郎, 村上敦史, 岩崎洋子, 三輪真由美, 渡辺建介
Organizer
第22回日本アレルギー学会春季臨床大会
Place of Presentation
京都市
Related Report
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[Presentation] 点鼻ステロイド薬の鼻粘膜バリア機能に対する効果2010
Author(s)
広瀬壮, 三輪正人, 中島規幸, 狩野信和, 山口晋太郎, 村上敦史, 阿部実恵子, 岩崎洋子, 蓮琢也, 呉松晃, 渡辺建介
Organizer
第22回日本アレルギー学会春季臨床大会
Place of Presentation
京都市
Related Report
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[Presentation] 抗酸化剤点鼻によるヒト鼻粘膜上皮バリア機能のup-regulation2009
Author(s)
三輪正人, 阿部実恵子, 中島規幸, 広瀬壮, 岩崎洋子, 山口晋太郎, 村上敦史, 蓮琢也, 呉松晃, 三輪真由美, 渡辺建介
Organizer
第59回日本アレルギー学会秋季学術大会
Place of Presentation
秋田
Related Report
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[Presentation] 保湿剤点鼻によるヒト鼻粘膜上皮バリア機能の変化とその持続時間2009
Author(s)
狩野信和, 三輪正人, 阿部実恵子, 中島規幸, 広瀬壮, 狩野信和, 呉松晃, 蓮琢也, 山口晋太郎, 村上敦史, 三輪真由美, 渡辺建介
Organizer
第59回日本アレルギー学会秋季学術大会
Place of Presentation
秋田
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