Roles of stromal cells in the survival, growth, differentiation, migration of laryngeal carcinoma-derived cancer stem cells and its mechanisms

• Project/Area Number: 20592023
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Saga University
• Principal Investigator: TODA Shuji Saga University, 医学部, 教授 (80188755)
• Co-Investigator(Kenkyū-buntansha): KURATOMI Yuichiro 佐賀大学, 医学部, 准教授 (30225247)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 喉頭癌 / 癌幹細胞 / 線維芽細胞 / 脂肪細胞 / 増殖 / アポトーシス / 遊走 / 浸潤

Research Abstract

Stromal fibroblasts promote the survival, self-renewal and cancer cell production of cancer stem cells under cancer stem cell-fibroblast interaction, while both adipocytes and adipose tissues tend to inhibit those of the cancer stem cells. Adipose tissue-derived free fatty acid, palmitate, tends to promote the apoptosis of cancer stem cells. Finally, the stromal-cancer stem cell interaction does not induce the significant changes ofadipokine production and gene expression.

Research Products

• [Journal Article] A promising culture model for analyzing the interaction between adipose tissue and cardiomyocytes. (2011)
  • Author(s): Anan M, Uchihashi K, Aoki S, Matsunobu A, Ootani A, Node K, Toda S.
  • Journal Title: Endocrinology 152 Pages: 1599-15609
  • Peer Reviewed
• [Journal Article] Interaction between adipose tissue stromal cells and gastric cancer cells in vitro. (2011)
  • Author(s): Nomoto-Kojima N, Aoki S, Uchihashi K, 松Matsunobu A, Koike E, Ootani A, Yonemitsu N, Fujimoto K, Toda S.
  • Journal Title: Cell Tissue Res 344 Pages: 287-298
  • Peer Reviewed
• [Journal Article] 脂肪組織と脂肪細胞の基礎形態学 (2011)
  • Author(s): 戸田修二、松延亜紀、内橋和芳、山本美保子、薬師寺舞、山崎文朗、小池英介、青木茂久、杉原甫
  • Journal Title: 臨床検査 55 Pages: 533-538
  • Peer Reviewed
• [Journal Article] 脂肪組織を基盤とした生体恒常性・メタボリックシンドローム病態解析モデル (2011)
  • Author(s): 松延亜紀、藤本一眞、戸田修二
  • Journal Title: 臨床検査 55 Pages: 581-586
  • Peer Reviewed
• [Journal Article] FLUID FLOW STRESS AFFECTS PERITONEAL CELL KINETICS : POSSIBLE PATHOGENESIS OF PERITONEAL FIBROSIS. (2011)
  • Author(s): Aoki S, Makino J, Nagashima A, Takezawa T, Nomoto N, Uchihashi K, Matsunobu A, Sanai T, Sugihara H, Toda S.
  • Journal Title: Perit Dial Int. Epub ahead of print
  • Peer Reviewed
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture. (2011)
  • Author(s): Udo K, Aoki S, Uchihashi K, Kawasaki M, Matsunobu A, Tokuda Y, Ootani A, Toda S, Uozumi J.
  • Journal Title: Kidney Int 79 Pages: 1151-1151
  • Peer Reviewed
• [Journal Article] Organotypic culture of human bone marrow adipose tissue. (2010)
  • Author(s): Uchihashi K, Aoki S, Shigematsu M, Kamochi N, Sonoda E, Soejima H, Fukudome K, Sugihara H, Hotokebuchi T, Toda S.
  • Journal Title: Pathol Int. 60(4) Pages: 259-267
  • NAID: 10029331372
  • Peer Reviewed
• [Journal Article] メタボリック症候群における脂肪組織の病理形態と病態解析培養モデル (2010)
  • Author(s): 戸田修二, 他8名
  • Journal Title: 病理と臨床 28 Pages: 959-965
  • Peer Reviewed
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture. (2010)
  • Author(s): Udo K, Aoki S, Uchihashi K, Kawasaki M, Matsunobu A, Tokuda Y, Ootani A, Toda S, Uozumi J.
  • Journal Title: Kidney Int 78 Pages: 60-68
  • Peer Reviewed
• [Journal Article] The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase. (2010)
  • Author(s): Yee CH, Aoki S, Uchihashi K, Matsunobu A, Yamasaki F, Misago N, Piao M, Tetsuji U, Yonemitsu N, Sugihara H, Toda S.
  • Journal Title: Acta Histochem Cytochem 43 Pages: 1-7
  • NAID: 130000158695
  • Peer Reviewed
• [Journal Article] メタボリック症候群における脂肪組織の病理形態と病態解析培養モデル (2010)
  • Author(s): 戸田修二, 他
  • Journal Title: 病理と臨床 Volume: 28 Pages: 959-965
  • Peer Reviewed
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture (2010)
  • Author(s): Udo K, Toda S, et al.
  • Journal Title: Kidney International Volume: 78 Pages: 60-68
  • Peer Reviewed
• [Journal Article] Organotypic culture of human bone marrow adipose tissue (2010)
  • Author(s): Uchihashi K, Toda S, et al
  • Journal Title: Pathology International Volume: 60 Pages: 259-267
  • NAID: 10029331372
  • Peer Reviewed
• [Journal Article] The air liquid interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase. 43:1-7, 2010 (2010)
  • Author(s): Yee CH, Toda S, et al
  • Journal Title: Acta Histochem Cytochem Volume: 43 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration. (2009)
  • Author(s): 戸田修二, 内橋和芳, 青木茂久, 他6名
  • Journal Title: Organogenesis 5 Pages: 50-56
  • Peer Reviewed
• [Journal Article] Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. (2009)
  • Author(s): Ootani A, Li X, Sangiorgi E, Ho QT, Ueno H, Toda S, 他
  • Journal Title: Nat Med 15 Pages: 701-706
  • Peer Reviewed
• [Journal Article] 甲状腺研究のための培養系 (2009)
  • Author(s): 戸田修二, 小池英介, 青木茂久, 他6名
  • Journal Title: ホルモンと臨床 57 Pages: 80-87
  • Peer Reviewed
• [Journal Article] Non-skin mesenchymal cell types support epidermal regeneration in a mesenchymal stem cell or myofibroblast phenotype-independent manner. (2009)
  • Author(s): Aoki S, Takezawa T, Uchihashi K, Sugihara H, Toda S.
  • Journal Title: Pathol Int 59 Pages: 368-375
  • NAID: 10027380333
  • Peer Reviewed
• [Journal Article] 成熟脂肪細胞、前脂肪細胞及び間葉系幹細胞を有する多機能性脂肪組織の長期培養法の確立 (2009)
  • Author(s): 園田恵美子, 内橋和芳, 青木茂久, 船津丸貞幸, 米満伸久, 戸田修二
  • Journal Title: ホルモンと臨床 57 Pages: 92-101
  • Peer Reviewed
• [Journal Article] An organotypic culture system of Merkel cells using isolated epidermal sheets. (2009)
  • Author(s): Nagase K, Aoki S, Uchihashi K, Misago N, Shimohira-Yamasaki M, Toda S, Narisawa Y.
  • Journal Title: Br J Dermatol 161 Pages: 1239-1247
  • Peer Reviewed
• [Journal Article] Adipose tissue-organotypic culture as a promising model for studying adipose tissue biology and regeneration (2009)
  • Author(s): Shuji Toda, et al.
  • Journal Title: Organogenesis 5 Pages: 50-56
  • Peer Reviewed
• [Journal Article] Sustained in vitro intestinal epithelial culture within a Wnt-dependentstem cell niche (2009)
  • Author(s): Akifumi Ootani, Shuji Toda, et al.
  • Journal Title: Nature Medicine 15 Pages: 701-706
  • Peer Reviewed
• [Journal Article] An organotypic culture system of Merkel cells using isolated epidermal sheets (2009)
  • Author(s): Kotaro Nagase, Shuji Toda, et al.
  • Journal Title: British Journal of Dermatology 161 Pages: 1239-1247
  • Peer Reviewed
• [Journal Article] Non-skin mesenchymal cell types support epidermal regeneration in a mesenchymal stem cell or myofibroblast phenotype-independent manner (2009)
  • Author(s): Shigehisa Aoki, Shuji Toda, et al.
  • Journal Title: Pathology International 59 Pages: 368-375
  • NAID: 10027380333
  • Peer Reviewed
• [Journal Article] A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells. (2008)
  • Author(s): Sonoda E, Aoki S, Uchihashi K, Soejima H, Kanaji S, Izuhara K, Satoh S, Fujitani N, Sugihara H, Toda S.
  • Journal Title: Endocrinology 149 Pages: 4794-4798
  • Peer Reviewed
• [Journal Article] Irradiated fibroblast-induced bystander effects on invasive growth of squamous cell carcinoma under cancer-stromal cell interaction. (2008)
  • Author(s): Kamochi N, Nakashima M, Aoki S, Uchihashi K, Sugihara H, Toda S, Kudo S.
  • Journal Title: Cancer Sci 99 Pages: 2417-2427
  • NAID: 10024014179
  • Peer Reviewed
• [Journal Article] 骨芽細胞とヒト骨髄脂肪組織の細胞間相互作用は骨髄のホメオスターシスに関与する (2008)
  • Author(s): 内橋和芳, 青木茂久, 園田恵美子, 米満伸久, 船津丸貞幸, 井手口浩幸, 杉原甫, 佛淵孝夫, 戸田修二
  • Journal Title: ホルモンと臨床 56 Pages: 170-176
  • Peer Reviewed
• [Journal Article] A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term. together with development of immature adipocytes and mesenchymal stem cell-like cells. (2008)
  • Author(s): Sonoda E, Aoki S, Uchihashi K, Soejima H, Kanaji S, Izuhara K, Satoh S, Fujitani N, Sugihara H. Todas.
  • Journal Title: Endocrinology 149 Pages: 4794-4798
  • Peer Reviewed
• [Journal Article] Culture model for studying thyroid biology and disorders.
  • Author(s): Toda S, Aoki S, Uchihashi K, Matsunobu A, Yamamoto M, Ootani A, Yamasaki F, Koike E, Sugihara H.
  • Journal Title: ISRN Endocrinology in press
  • Peer Reviewed
• [Journal Article] The influence of the skin microenvironment air-liquid interface on melanoma. Melanoma Book-1
  • Author(s): 戸田修二, 青木茂久, 内橋和芳, 松延亜紀, 薬師寺舞
  • Journal Title: Ed.by Murph M.InTech-Open access publisher in press
  • Peer Reviewed
• [Presentation] Adipose tissue-organotypic culture and its application for studying adipose tissue biology. (2010)
  • Author(s): 戸田修二
  • Organizer: 28^<th> Japan Endocrine Society Summer Seminar on Endocrinology & Metabolis(招待講演)
  • Place of Presentation: 佐世保市
  • Year and Date: 2010-07-10
• [Presentation] Adipose tissue-organotypic culture and its application for studying adipose tissue biology (2010)
  • Author(s): Toda S
  • Organizer: 28^<th> Japan Endocrine Society Summer Seminar on Endocrinology & Metabolis
  • Place of Presentation: 佐世保市、ハウステンボス
  • Year and Date: 2010-07-10
• [Presentation] 組織細胞化学に於ける転写解析:各種培養系における細胞増殖・分化と情報伝達・転写因子の発現 (2008)
  • Author(s): 戸田修二
  • Organizer: 第49回日本組織細胞化学会総会(招待講演)
  • Place of Presentation: 長崎市
  • Year and Date: 2008-10-10
• [Remarks] ホームページ等