| Project/Area Number |
20592047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
YOSHIKAWA Hiroshi Kyushu University, 大学病院, 助教 (00304808)
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| Co-Investigator(Kenkyū-buntansha) |
YONEMITSU Yoshikazu 九州大学, 大学院・薬学研究院, 客員教授 (40315065)
園田 康平 山口大学, 医学部, 教授 (10294943)
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| Co-Investigator(Renkei-kenkyūsha) |
SONODA Koh-hei 九州大学, 大学病院, 講師 (10294943)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 眼細胞生物学 / 眼腫瘍学 / 網膜芽細胞腫 / 遺伝子治療 / 免疫治療 / SeV / ΛM / 硝子体播種 / ΔM |
|---|
| Research Abstract |
We investigated weather anti-tumor gene therapy using M gene deficient Sendai virus vector encoding IFN-β gene (SeV/ΔM-IFN-β) has therapeutic potentials for the treatment of retinoblastoma. Whereas the gene transduction efficiency of SeV/ΔM vector is low in retinoblastoma cell lines, it mediates efficient gene transfer in a retinal pigment epithelium (RPE)-derived cell line. SeV/ΔM-mediated overexpression of IFN-β in RPE suppressed the growth of retinoblastoma cells, suggesting that intraocular IFN-β gene transfer using SeV/ΔM vector may be a useful strategy for preventing tumor growth in retinoblastoma patients.
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