The Development of the regenerated mucosal epithelial transplantation for immunomodulation and ocular surface reconstruction.
| Project/Area Number |
20592054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
INATOMI Tsutomu Kyoto Prefectural University of Medicine, 医学研究科, 助教 (00305583)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Satoshi 京都府立医科大学, 医学研究科, 助教 (60347458)
MARUYAMA Kazuichi 京都府立医科大学, 医学研究科, 助教 (10433244)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 再生医療 / 角膜上皮 / 口腔粘膜上皮 / 血管新生 / 眼表面再建 / 口腔粘膜 / ムチン / 粘膜免疫 |
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| Research Abstract |
We have successfully generated cultivated corneal epithelial sheet and oral mucosal epithelial sheet which are applicable for ocular surface reconstruction. These epithelial sheets expressed p75 in the basal region suggesting the high potential for cell proliferation. Ectopicallysurvived oral mucosal epithelium on the cornea expressed neither PAX6 gene nor K1 and K10. However,these cells expressed K4, K3 and K13, but not K12. The phenotype of surviving transplanted oral mucosal epithelium on the cornea was the same as in vivo oral mucosal epithelium, suggesting that cultivated oral mucosal epithelium does not transdifferentiate into corneal epithelium. Peripheralneovascularization and lymphanogenesis were induced in early post-operative phase. In vivo confocal microscopic analysis revealed well-organized stratified epithelium containing the proper cell density of basal cells and flattened apical cells. Anti-VEGF treatment could inhibit the progression of vessels and control the phenotype of surviving epithelial cells on the ocular surface.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Basophils in the giant papillae of chronic allergic keratoconjunctivitis.2010
Author(s)
Matsuda A, Ebihara N, Yokoi N, Okayama Y, Watanabe Y, Kawasaki S, Tanioka H, Walls AF, Hamuro J, Kinoshita S, Murakami A.
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Journal Title
Br J Ophthalmol. 94
Pages: 513-518
Related Report
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[Journal Article] Association between prostaglandin E receptor 3 polymorphisms and Stevens-Johnson syndrome identified by means of a genome-wide association study.2010
Author(s)
Ueta M, Sotozono C, Nakano M, Taniguchi T, Yagi T, Tokuda Y, Fuwa M, Inatomi T, Yokoi N, Tashiro K, Kinoshita S.
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Journal Title
J Allergy Clin Immunol. 126(6)
Pages: 1218-1225
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[Journal Article] Cultivated human conjunctival epithelial transplantation for total limbal stem cell deficiency.2010
Author(s)
Ang LP, Tanioka H, Kawasaki S, Ang LP, Yamasaki K, Do TP, Thein ZM, Koizumi N, Nakamura T, Yokoi N, Komuro A, Inatomi T, Nakatsukasa M, Kinoshita S.
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Journal Title
Invest Ophthalmol Vis Sci. 51(2)
Pages: 758-764
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