A Device for Gene Transfer to the Anterior Segment of the Eye Using Anionic Hydrogel Contact Lens

• Project/Area Number: 20592061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Juntendo University
• Principal Investigator: FUJIMAKI Takuro Juntendo University, 医学部, 准教授 (50333042)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Akira 順天堂大学, 医学部, 教授 (90157743) ; 土至田 宏 順天堂大学, 医学部, 准教授 (00306961)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ソフトコンタクトレンズ / プラスミドDNA / 培養ヒト角膜上皮細胞 / 遺伝子導入

Research Abstract

To investigate non-viral device for gene transfer to the anterior segment of the eye using a newly developed anionic hydrogel soft contact lens (SCL). The newly developed SCL which contained imidazolium group and phosphate group in its side chains lens might be one of the efficient devices for non-viral gene transfection to corneal epithelial cells. It is suggested that this SCL could be applied for gene therapy for corneal diseases.

Research Products

• [Presentation] A Device for Gene Transfer to the Anterior Segment of the Eye Using Anionic Hydrogel Contact Lens. (2010)
  • Author(s): T.Matsunaga, T.Sato, Y Watanabe, T.Fujimaki, A.Murakami
  • Organizer: ARVO 2010
  • Place of Presentation: Florida, U.S.A.
  • Year and Date: 2010-05-04
• [Presentation] A Device for Gene Transfer to the Anterior Segment of the Eye Using Anionic Hydrogel Contact Lens. (2010)
  • Author(s): T.Matsunaga
  • Organizer: ARVO 2010
  • Place of Presentation: Florida
  • Year and Date: 2010-05-04
• [Presentation] Gene Introduction to the Corneal Epithelial Cells with a Calcium Phosphate Complex Contact Lens. (2009)
  • Author(s): T.Sato, Y Watanabe, T.Fujimaki, A.Murakami
  • Organizer: Asia Pacific Academy of Ophthalmology
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2009-05-19
• [Presentation] Gene Introduction to the Corneal Epithelial Cells with a Calcium Phosphate Complex Contact Lens (2009)
  • Author(s): T.Matsunaga
  • Organizer: Asia Pacific Academy of Ophthalmology
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2009-05-19
• [Presentation] リン酸カルシウム複合体コンタクトレンズによる培養角膜上皮細胞への遺伝子導入 (2009)
  • Author(s): 藤巻拓郎、松永透、佐藤隆郎、村上晶
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-15
• [Patent(Industrial Property Rights)] 遺伝子治療に用いられるハイドロゲル製眼用レンズ (2008)
  • Inventor(s): 佐藤隆郎、松永透、藤巻拓郎、村上晶
  • Industrial Property Rights Holder: 佐藤隆郎、松永透、藤巻拓郎、村上晶
  • Patent Publication Number: 2008-220823
  • Filing Date: 2008-09-25
• [Patent(Industrial Property Rights)] 眼の遺伝子治療に用いられるハイドロゲル製眼用レンズ (2007)
  • Inventor(s): 佐藤隆郎、宇野憲治、藤巻拓郎、村上晶
  • Industrial Property Rights Holder: 佐藤隆郎、宇野憲治、藤巻拓郎、村上晶
  • Patent Publication Number: 2007-187897
  • Filing Date: 2007-07-26