| Project/Area Number |
20592063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
YOSHITAKE Yoshino Kanazawa Medical University, 医学部, 准教授 (00150764)
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| Co-Investigator(Kenkyū-buntansha) |
YONEKURA Hidoto 金沢医科大学, 医学部, 教授 (80240373)
YAGUCHI Hiromoto 金沢医科大学, 医学部, 助教 (40410344)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 血管内皮細胞 / miRNA / 増殖性網膜症 / sFlt-1 / レンチウイルス / 白内障 / UVB / 水晶体上皮細胞 / 管腔形成 / 低酸素 / レンチウイルスベクター / mRNA / マイクロアレイ / Dicer / siRNA / miRNAマイクロアレイ |
|---|
| Research Abstract |
When human microvascular endothelial cells (HMVEC), a constituent of microvessels wherein angiogenesis occurs in diseases, formed the capillary like tubes on plastic plate, we found the expression of some specific genes and micro RNA (small non-coding RNA). Hypoxia (2.5%O_2 ) down-regulated anti-angiogenic factor, sFlt-1 while it up-regulated angiogenic factor, VEGF in HMVEC. UV exposure to human lens epithelial cells stimulated the secretion of several cytokines, which may result in cataract. Our research might be useful for diminishing the actions of causing-disease genes.
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