Microarray analysis of proliferative vitreoretinopathy to identify molecular targets for new diagnosis and therapy

• Project/Area Number: 20592066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Fukuoka University
• Principal Investigator: YOSHIDA Shigeo Fukuoka University, 大学病院, 講師 (50363370)
• Co-Investigator(Kenkyū-buntansha): ISHIBASHI Tatsuro 九州大学, 医学研究院, 教授 (30150428) ; KONO Toshihiro 福岡大学, 医学部, 教授 (40161880) ; 向野 利寛 福岡大学, 筑紫病院, 教授 (40117106)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ゲノム医科学 / 増殖硝子体網膜症 / 遺伝子発現 / マイクロアレイ

Research Abstract

We performed global gene expression analysis using epiretinal membranes (ERMs) from patients with proliferative vitreoretinopathy (PVR) and mouse model of ischemic retinopathy. Genes preferentially expressed in ERMs were subdivided by the functional sets of genes such as oxidative phosphorylation, cell adhesion, cell communication, cytokine-receptor association. Periostin was expressed in ERMs which located in smooth muscle actin-positive cells. In mouse model of ischemic retinopathy, MIP1β was upregulated in ischemic inner retina and was associated with physiological retinal revascularization.

Research Products

• [Journal Article] Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ogura A, Ishikawa K, Yoshida A, Kohno R, Yamaji Y, et al.
  • Journal Title: Br J Ophthalmol 94 Pages: 795-801
• [Journal Article] Gene expression profile of hyperoxic and hypoxic retinas in a mouse model of oxygen-induced retinopathy. (2010)
  • Author(s): Ishikawa K, Yoshida S, Kadota K, Nakamura T, Niiro H, Arakawa S, et al.
  • Journal Title: Invest Ophthalmol Vis Sci 51 Pages: 4307-4319
• [Journal Article] Reduced concentrations of angiogenesis-related factors in vitreous after vitrectomy in patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ishikawa K, Matsumoto T, Yoshida A, Ishibashi T, Kono T
  • Journal Title: Graefes Arch Clin Exp Ophthalmol 248 Pages: 799-804
• [Journal Article] Gene expression profile of hyperoxic and hypoxic retinas in a mouse model of oxygen-induced retinopathy (2010)
  • Author(s): Ishikawa K, Yoshida S
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51 Pages: 4307-4319
  • Peer Reviewed
• [Journal Article] Reduced concentrations of angiogenesis-related factors in vitreous after vitrectomy in patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ishikawa K, Matsumoto T, Yoshida A, Ishibashi T, Kono T.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. Volume: 248 Pages: 799-804
  • Peer Reviewed
• [Journal Article] Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ogura A, Ishikawa K, Yoshida A, Kohno R, Yamaji Y, Ikeo K, Gojobori T, Kono T, Ishibashi T.
  • Journal Title: Br J Ophthalmol. Volume: 94 Pages: 795-801
  • Peer Reviewed
• [Journal Article] Case of novel PITX2 gene mutation associated with Peters' anomaly and persistent hyperplastic primary vitreous. (2010)
  • Author(s): Arikawa A, Yoshida S, Yoshikawa H, Ishikawa K, Yamaji Y, Arita RI, Ueno A, Ishibashi T.
  • Journal Title: Eye (Lond). Volume: 24 Pages: 391-393
  • Peer Reviewed
• [Journal Article] 網膜上組織の包括的遺伝子発現解析により治療の新しい標的を同定できる (2010)
  • Author(s): 吉田茂生, 石川桂二郎
  • Journal Title: 福岡県眼科医会報 Volume: 212 Pages: 3-9
  • Peer Reviewed
• [Journal Article] 臨床医学の展望 角膜 (2010)
  • Author(s): 吉田茂生, 畑快右, 石橋達朗
  • Journal Title: 日本医事新報 Pages: 73-80
  • Peer Reviewed
• [Journal Article] Clinical Course during Forty-year Follow-up of Axenfeld-Rieger Syndrome in a Japanese Family (2010)
  • Author(s): Yoshida S
  • Journal Title: Oman J Ophthalmol 3 Pages: 34-35
  • Peer Reviewed
• [Journal Article] Gene Expression Profile of Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy (2009)
  • Author(s): Yoshida S
  • Journal Title: Br J Ophthalmol.2010 (in press)
  • Peer Reviewed
• [Journal Article] VMD2 Mutational Analysis in Japanese Family with Best Macular Dystrophy (2009)
  • Author(s): Shiose S, Yoshida S
  • Journal Title: Oman J Ophthalmol 2009 2 Pages: 143-144
  • Peer Reviewed
• [Journal Article] TEM7 (PLXDC1) in neovascular endothelial cells of fibrovascular membranes from patients with proliferative diabetic retinopathy. (2008)
  • Author(s): Yamaji Y, Yoshida S
  • Journal Title: Invest Ophthalmol Vis Sci 49(7) Pages: 3151-3157
  • Peer Reviewed
• [Journal Article] 眼の感染と免疫、眼炎症と自然免疫 (2008)
  • Author(s): 園田康平, 吉田茂生
  • Journal Title: 日本眼科学会雑誌 112(3) Pages: 279-298
  • NAID: 10021292215
  • Peer Reviewed
• [Journal Article] 網膜中心静脈閉塞症を合併した49歳過熟白内障の1例 (2008)
  • Author(s): 山中時子, 吉田茂生
  • Journal Title: 眼科臨床紀要 1 Pages: 21-24
  • NAID: 10024806182
  • Peer Reviewed
• [Journal Article] 多局所ERGと多局所VEPによる視機能評価 (2008)
  • Author(s): 後藤純信, 吉田茂生
  • Journal Title: 臨床脳波 50 Pages: 79-86
  • Peer Reviewed
• [Presentation] Alteration of the balance between vascular endothelial growth factor and endostatin in vitreous after vitrectomy in patients with proliferative diabetic retinopathy. (2011)
  • Author(s): 吉田茂生
  • Organizer: ASIA PACIFIC ACADEMY OF OPHTHALMOLOGY CONGRESS
  • Place of Presentation: SYDNEY
  • Year and Date: 2011-03-24
• [Presentation] Alteration of the balance between vascular endothelial growth factor and endostatin in vitreous after vitrectomy in patients with proliferativediabetic retinopathy. (2011)
  • Author(s): Yoshida S
  • Organizer: ASIA PACIFIC ACADEMY OF OPHTHALMOLOGY CONGRESS
  • Place of Presentation: SYDNEY
  • Year and Date: 2011-03-24
• [Presentation] 増殖糖尿 病網膜症の硝子体手術前後の硝子体中VEGFの低下:同一 症例での検討 (2010)
  • Author(s): 吉田茂生, 石川桂二郎, 向野利寛, et al.
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-11-26
• [Presentation] 増殖糖尿病網膜症の硝子体手術前後の硝子体中VEGFの低下:同一症例での検討 (2010)
  • Author(s): 吉田茂生, 石川桂二郎, 向野利寛, et al
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-11-26
• [Presentation] The concentration changes of VEGF and MCP-1 in the eyes of the patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Sassa Y, Asato R, Ishikawa K, Saeki Y, Kono T
  • Organizer: (The second vitrectomy cases). The Third Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: Beijing
  • Year and Date: 2010-09-14
• [Presentation] Reduced concentrations of VEGF in vitreous after vitrectomy in patients with proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ishikawa K, Yukio S, Ryo A, Ayako Y, Kono T
  • Organizer: In : The Third Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: Beijing
  • Year and Date: 2010-09-14
• [Presentation] The concentration changes of VEGF and MCP-1 in the eyes of the patientswith proliferative diabetic retinopathy.(The second vitrectomy cases) (2010)
  • Author(s): Yoshida S, Sassa Y, Asato R, Ishikawa K, Saeki Y, Kono T
  • Organizer: The Third Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: Beijing
  • Year and Date: 2010-09-14
• [Presentation] Reduced concentrations of VEGF in vitreous after vitrectomy in patientswith proliferative diabetic retinopathy. (2010)
  • Author(s): Yoshida S, Ishikawa K, Yukio S, Ryo A, Ayako Y, Kono T
  • Organizer: The Third Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: Beijing
  • Year and Date: 2010-09-14
• [Presentation] 最近2年間の増殖糖尿病網膜症に対する硝子体手術成績 (2010)
  • Author(s): 吉田茂生, 松本時子, 鍋島崇寛, 野田美登利, 佐伯有祐, 向野利寛
  • Organizer: 九州眼科学会
  • Place of Presentation: 佐賀市
  • Year and Date: 2010-05-28
• [Presentation] Expressed Sequence Tag Analysis of Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy. (2010)
  • Author(s): Yoshida S
  • Organizer: Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-06
• [Presentation] 増殖糖尿病網膜症にともなう線維血管増殖組織におけるペリオスチンの発現上昇 (2010)
  • Author(s): 吉田茂生, 石川桂二郎, 樋口直美, 向野利寛, 石橋達朗
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15
• [Presentation] マイクロアレイを用いた網膜上増殖組織の包括的遺伝子発現解析 (2009)
  • Author(s): 吉田茂生
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-12-04
• [Presentation] Persistent secretion of Endostatin in Vitreous after Vitrectomy in Patients with Proliferative Diabetic Retinopathy (2009)
  • Author(s): 吉田茂生
  • Organizer: The Second Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: Fukuoka
  • Year and Date: 2009-11-29
• [Presentation] 増殖糖尿病網膜症の硝子体手術前後の硝子体中 endostatin の変化 (2009)
  • Author(s): 吉田茂生
  • Organizer: 日本臨床眼科学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-10-11
• [Presentation] 増殖糖尿病網膜症の硝子体手術前後の硝子体中MCP-1の変化 (2009)
  • Author(s): 吉田茂生
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-04-18
• [Presentation] 硝子体手術後の糖尿病網膜症患者硝子体中の血管新生因子の変化 (2008)
  • Author(s): 吉田茂生
  • Organizer: 日本網膜硝子体学会
  • Place of Presentation: 京都市
  • Year and Date: 2008-11-29
• [Presentation] 硝子体手術による糖尿病網膜症患者硝子体中の血管新生因子の変化 (2008)
  • Author(s): 吉田茂生
  • Organizer: ジャパンマクラクラブ
  • Place of Presentation: 蒲郡
  • Year and Date: 2008-08-23
• [Presentation] ゲノム医科学からみた網膜前増殖組織の生成機序 (2008)
  • Author(s): 古田茂生
  • Organizer: 第10回かぼす眼科研究会
  • Place of Presentation: 別府
  • Year and Date: 2008-06-14
• [Presentation] Role of TEM7 in Neovascular Endothelial Cells of Fibrovascular Membranes From Patients With Proliferative Diabetic Retinopathy (2008)
  • Author(s): Yoshida S
  • Organizer: Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2008-04-27
• [Presentation] 増殖硝子体網膜症に伴う増殖組織のexpressed sequence tag解析 (2008)
  • Author(s): 吉田茂生
  • Organizer: 日本眼科学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-04-17
• [Remarks] ホームページ等
  • URL: http://www.eye.med.kyushu-u.ac.jp/
• [Patent(Industrial Property Rights)] 増殖糖尿病網膜症の検出方法および予防・治 療剤のスクリーニング方法 (2010)
  • Inventor(s): 吉田茂生、出原賢治
  • Industrial Property Rights Holder: 福岡大学、佐賀大学
  • Industrial Property Number: 2010-093240
  • Filing Date: 2010-04-09
• [Patent(Industrial Property Rights)] 増殖糖尿病網膜症の検出方法および予防・治療剤のスクリーニング方法 (2010)
  • Inventor(s): 吉田茂生、出原賢治
  • Industrial Property Rights Holder: 福岡大学、佐賀大学
  • Industrial Property Number: 2010-093240
  • Filing Date: 2010-04-09