Proteomic and Lipidomic Analysis of Tear Fluids from Dry Eye Patients
| Project/Area Number |
20592068
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | 独立行政法人国立病院機構(東京医療センター臨床研究センター) |
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Principal Investigator |
YAMADA Masakazu 独立行政法人国立病院機構(東京医療センター臨床研究センター), 東京医療センター臨床研究センター視覚研究部, 部長 (50210480)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 蛋白質 / プロテオーム / 分析化学 / 生体分子 / 脂質 |
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| Research Abstract |
To understand the pathophysiology of dry eye syndrome, there is a need for precise and intensive biochemical analysis of tear fluids. We employed liquid chromatography combined with electron-spray ionization mass spectroscopy to profile tear proteins and lipids from tears of patients with dry eye syndrome. Hundreds of various molecules were successfully identified from a small volume of tear sample. Of these, prominent differences were found in the scores of complement C3, phosphatidylcholine, and sphingomyelin. The score of complement C3 in patients with dry eye syndrome was much higher than that of normal subjects. The levels of phosphatidylcholine and sphingomyelin in tears of dry eye syndrome were significantly lower than those of normal controls. Proteomic and lipidomic approaches appear to be useful in exploring the biomarkers of dry eye and the new potential targets for the treatments of dry eye syndrome.
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Report
(4 results)
Research Products
(136 results)
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[Journal Article] mUBIAD1mmuta tionzalterszammitochondrial prenyltrans ferase z to cause Schnyder corneal dyst rophy.2010
Author(s)
Nickerson ML, Kostiha BN, Brandt W, Fredericks W, Xu KP, Yu FS, Gold B, Chodosh J, Goldberg M, Lu da W, Yamada M, Tervo TM, Grutzmacher R, Croasdale C, Hoeltzenbein M, Sutphin J, Malkowicz SB, Wessjohann L, Kruth HS, Dean M, Weiss JS.
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Peer Reviewed
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[Journal Article] マイボーム腺機能不全ワーキンググループ.マイボーム腺機能不全の定義と診断基準2010
Author(s)
天野史郎, 有田玲子, 木下茂, 横井則彦, 外園千恵, 小室青, 鈴木智, 島崎潤,田聖花, 前田直之, 高静花, 堀裕一, 西田幸二, 久保田久世, 後藤英樹, 山口昌彦, 小幡博人, 山田昌和, 村戸ドール, 小川葉子, 松本幸裕, 坪田一男
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Journal Title
あたらしい眼科 27
Pages: 627-631
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Peer Reviewed
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[Journal Article] UBIAD1 mutation alters a mitochondrial prenyltransferase to cause Schnyder corneal dystrophy2010
Author(s)
Nickerson ML, Kostiha BN, Brandt W, Fredericks W, Xu KP, Yu FS, Gold B, Chodosh J, Goldberg M, Lu da W, Yamada M, Tervo TM, Grutzmacher R, Croasdale C, Hoeltzenbein M, Sutphin J, Malkowicz SB, Wessjohann L, Kruth HS, Dean M, Weiss JS.
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[Journal Article]2009
Author(s)
山田昌和
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Journal Title
こどもの目やに、抗菌薬でいいの? 眼科インストラクションコース18. 眼科診療のスキルアップ、白内障・小児・ぶどう膜炎編(メジカルビュー社)
Pages: 123-127
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[Journal Article]2009
Author(s)
重安千花、山田昌和
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Journal Title
コンタクトレンズと角膜代謝. 眼科プラクテイス28、標準コンタクトレンズ診療(文光堂)
Pages: 37-40
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[Journal Article]2009
Author(s)
山田昌和、水野嘉信
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Journal Title
アクネ菌角膜炎. 眼科プラクテイス29、眼感染症の謎を解く(文光堂)
Pages: 134-135
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[Journal Article]2009
Author(s)
山田昌和
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Journal Title
ニューキノロン製剤の薬剤移行比較. 眼科プラクティス29、眼感染症の謎を解く(文光堂)
Pages: 430-431
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[Journal Article] 拒絶反応のない理想的な角膜移植手術を目指して全層角膜移植から内皮細胞移植へ2008
Author(s)
山上聡, 新家眞, 天野史郎, 臼井智彦, 三村達哉, 横尾誠一, 青山佳世, 大沢稔也, 上羽悟史, 松島綱治, 林孝彦, 田中香純, 水木信久, 海老原伸行, 村上晶, 諸星計, 宮崎大, 井上幸次, HamrahP, LiuY, DanaMR, 中野英樹, 羽藤晋, 山田昌和, 羽室淳爾, 山田潤, 後藤晋, 小杉正明, 鈴木洋, 北川全
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