Transdifferentiation induction of murine epidermal SP cells into corneal epithelial cells.

• Project/Area Number: 20592079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Ehime University
• Principal Investigator: SHIRAISHI Atsushi Ehime University, 大学院・医学系研究科, 寄附講座准教授 (90314963)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Takeshi 愛媛大学, 大学院・医学系研究科, 寄附講座助教 (70380285)
• Co-Investigator(Renkei-kenkyūsha): OHASHI Yuichi 愛媛大学, 大学院・医学系研究科, 教授 (00116005) ; SHIRAKATA Yuji 愛媛大学, 大学院・医学系研究科, 講師 (50226320)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 眼発生 / 再生医学 / 角膜上皮 / 再生医療 / マウス表皮細胞 / 形質転換 / マウス角膜上皮細胞 / ケラチン12 / マウス角膜 / 培養

Research Abstract

In the present study, we investigated the potential for murine epidermal cells transdifferentiation into corneal epithelial-like cells, and demonstrate that murine epidermal cells have the potential to transdifferentiate into corneal epithelial-like cells. The results indicate that transdifferentiation may require the effect of coculture with corneal epithelial cells, as well as the corneal limbal environment.

Research Products

• [Journal Article] Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. (2010)
  • Author(s): Yoshioka R, Shiraishi A, Kobayashi T, Morita S, Hayashi Y, Higashiyama S, Ohashi Y.
  • Journal Title: Invest Ophthalmol Vis Sci. 51(11) Pages: 5630-5639
• [Journal Article] Monoallelic expression of Krt12 gene during corneal-type epithelium differentiation of limbal stem cells. (2010)
  • Author(s): Hayashi Y, Call MK, Liu CY, Hayashi M, Babcock G, Ohashi Y, Kao WW.
  • Journal Title: Invest Ophthalmol Vis Sci. 51(9) Pages: 4562-4568
• [Journal Article] Lumican is required for neutrophil extravasation following corneal injury and wound healing. (2010)
  • Author(s): Hayashi Y, Call MK, Chikama T, Liu H, Carlson EC, Sun Y, Pearlman E, Funderburgh JL, Babcock G, Liu CY, Ohashi Y, Kao WW.
  • Journal Title: J Cell Sci. 123(Pt17) Pages: 2987-2995
• [Journal Article] Role of insulin in regulation of Na+/K+-dependent ATPase activity and pump function in corneal endothelial cells. (2010)
  • Author(s): Hatou S, Yamada M, Akune Y, Mochizuki H, Shiraishi A, Joko T, Nishida T, Tsubota K.
  • Journal Title: Invest Ophthalmol Vis Sci. 51(8) Pages: 3935-3942
• [Journal Article] Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. (2010)
  • Author(s): Yoshioka R, Shiraishi A, Kobayashi T, Morita S, Hayashi Y, Higashiyama S, Ohashi Y.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51 Pages: 5630-39
  • Peer Reviewed
• [Journal Article] Role of insulin in regulation of Na+-/K+-dependent ATPase activity and pump function in corneal endothelial cells. (2010)
  • Author(s): Hatou S, Yamada M, Akune Y, Mochizuki H, Shiraishi A, Joko T, Nishida T, Tsubota K
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51 Pages: 3935-42
  • Peer Reviewed
• [Journal Article] Hypoxia-altered signaling pathways of toll-like receptor 4 (TLR4) in human corneal epithelial cells. (2009)
  • Author(s): Hara Y, Shiraishi A, Ohashi Y.
  • Journal Title: Mol Vis. 15 Pages: 2515-2520
• [Journal Article] New technique for culturing corneal epithelial cells of normal mice. (2009)
  • Author(s): Kobayashi T, Yoshioka R, Shiraishi A, Ohashi Y.
  • Journal Title: Mol Vis. 15 Pages: 1589-1593
• [Journal Article] Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial ells to viral infections. (2009)
  • Author(s): Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, Ohashi Y.
  • Journal Title: Mol Vis. 15 Pages: 937-948
• [Journal Article] The effects of dexamethasone on the Na, K-ATPase activity and pump function of corneal endothelial cells. (2009)
  • Author(s): Hatou S, Yamada M, Mochizuki H, Shiraishi A, Joko T, Nishida T.
  • Journal Title: Curr Eye Res. 34(5) Pages: 347-354
• [Journal Article] New technique for culturing corneal epithelial cells of normal mice (2009)
  • Author(s): Kobayashi T, Yoshioka R, Shiraishi A, Ohashi Y
  • Journal Title: Molecular Vision Aug 14;15 Pages: 1589-93
  • Peer Reviewed
• [Journal Article] Alteration of TLR3 pathways by glucocorticoids may be responsible for immunosusceptibility of human corneal epithelial cells to viral infections (2009)
  • Author(s): Hara Y, Shiraishi A, Kobayashi T, Kadota Y, Shirakata Y, Hashimoto K, Ohashi Y
  • Journal Title: Molecular Vision May 8;15 Pages: 937-4
  • Peer Reviewed
• [Journal Article] Hypoxia-altered signaling pathways of toll-like receptor 4(TLR4)in human corneal epithelial cells (2009)
  • Author(s): Hara Y, Shiraishi A, Ohashi Y
  • Journal Title: Molecular Vision Dec 2;15 Pages: 2515-20
  • Peer Reviewed
• [Journal Article] Promiscuous recombination of LoxP alleles during gametogenesis in cornea Cre driver mice. (2008)
  • Author(s): Weng DY, Zhang Y, Hayashi Y, Kuan CY, Liu CY, Babcock G, Weng WL, Schwemberger S, Kao WW.
  • Journal Title: Mol Vis. 14 Pages: 562-571
• [Presentation] 角膜上皮創傷治癒機転における epiregulin の役割の検討 (2010)
  • Author(s): 張媛, 白石敦 小林剛, 吉岡龍治, 林康人, 大橋裕一
  • Organizer: 第34回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-02-12
• [Presentation] Epithelial sheets produced from cryooreserved human corneal epithelial cells using dermal fibroblast-embedded collagen gel. (2010)
  • Author(s): 白石敦, 小林剛, 楊旅軍, 白方裕司, 橋本公二, 大橋裕一
  • Organizer: The 25^<th> APAO Congress - A Joint Meeting of APAO/AAO
  • Place of Presentation: 北京、中国
• [Presentation] 培養マウス表皮細胞から角膜上皮様細胞への形質転換の検討 (2010)
  • Author(s): 小林剛、林康人、白石敦、大橋裕一
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
• [Presentation] 凍結保存細胞によるヒト培養角膜上皮シートの作製 (2010)
  • Author(s): 小林剛、白石敦、楊旅軍、白方裕司、橋本公二、大橋裕一
  • Organizer: 第34回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 仙台
• [Presentation] Murine Epidermal Cells can be Transdifferentiated into Corneal Epithelial-like Cells in the Corneal Epithelial Environment (2010)
  • Author(s): Atsushi Shiraiahi, Takeshi Kobayashi, Yasuhito Hayashi, Yuichi Ohashi
  • Organizer: The Association for Research in Vision and Ophthalmology Annual Meeting
  • Place of Presentation: Fort Lawuderdale, USA
• [Presentation] Epithelial sheets produced from cryooreserved human corneal epithelial cells using dermal fibroblast-embedded collagen gel (2010)
  • Author(s): Atsushi SHIRAISHI, Takeshi KOBAYASHI, Lujun YANG, Yuji SHIRAKATA, Koji HASHIMOTO, Yuichi OHASHI
  • Organizer: The 25^<th> APAO Congress-A Joint Meeting of APAO/AAO
  • Place of Presentation: 北京,中国
• [Presentation] Role of HB-EGF in corneal epithelial wound healing (2009)
  • Author(s): R.Yoshioka, A.Shiraishi, T.Kobayashi, S.Morita, Y.Hayashi, S.Higashiyama, Y.Ohashi
  • Organizer: Korea Japan Joint Cornea Conference
  • Place of Presentation: 京都
  • Year and Date: 2009-10-17
• [Presentation] Role of HB-EGF in Corneal Epithelial Wound healing. (2009)
  • Author(s): 吉岡龍治, 森田真一, 林康人, 小林剛, 白石敦, 東山繁樹, 大橋裕一
  • Organizer: Korea Japan Joint Cornea Conference 2009
  • Place of Presentation: 京都
• [Presentation] HB-EGFはマウス角膜上皮細胞の遊走と接着を促進する (2009)
  • Author(s): 吉岡龍治, 白石敦, 小林剛, 森田真一, 林康人, 東山繁樹, 大橋裕一
  • Organizer: 第33回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 大阪
• [Presentation] コラーゲンゲルを用いた三次元上皮培養におけるゲル内実質細胞の増殖因子発現パターン (2009)
  • Author(s): 小林剛, 白石敦, 楊旅軍, 白方裕司, 橋本公二, 大橋裕一
  • Organizer: 第33回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 大阪
• [Presentation] コラーゲンゲルを用いた三次元上皮培養におけるゲル内実質細胞の増殖因子発現パターン (2009)
  • Author(s): 小林剛, 白石敦, 楊旅軍, 白方裕司, 橋本公二, 大橋裕一
  • Organizer: 第33回角膜カンファランス
  • Place of Presentation: 大阪
• [Presentation] コラーゲンゲルを用いた角膜上皮培養系における上皮-実質問相互作用の検討 (2009)
  • Author(s): 小林剛, 白石敦, 楊旅軍, 白方裕司, 橋本公二, 大橋裕一
  • Organizer: 第8回日本再生医療学会
  • Place of Presentation: 東京
• [Presentation] 眼科再生医療研究会-コラーゲンゲルを用いた培養ヒト角膜上皮シートの作製とその評価 (2008)
  • Author(s): 小林剛, 白石敦, 原祐子, 門田裕子, 楊旅軍, 白方裕司, 橋本公二, 大橋裕一
  • Organizer: 第62回日本臨床眼科学会
  • Place of Presentation: 東京
• [Presentation] Role of HB-EGF in corneal epithelial wound healing in vivo and in vitro. (2008)
  • Author(s): 吉岡龍治, 白石敦, 小林剛, 森田真一, 林康人, 東山繁樹, 大橋裕一
  • Organizer: The Association for Research in Vision and Ophthalmology Annual Meeting
  • Place of Presentation: Fort Lauderdale, USA
• [Presentation] Cultivation of cornea epithelial cells on the collagen gel containing human dermal fibroblasts. (2008)
  • Author(s): A.Shiraishi, T.Kobayashi, Y.Kadota, Y.Hara, Y.Shirakata, K.Hashimoto, Y.Ohashi
  • Organizer: Association for Research in Vision and Ophthalmology Annual Meeting
  • Place of Presentation: Fort Lauderdale, USA
• [Presentation] 角膜上皮創傷治癒機転におけるHB-EGFの役割-in vitro検討 (2008)
  • Author(s): 吉岡龍治, 白石敦, 森田真一, 小林剛, 林康人, 東山繁樹, 大橋裕一
  • Organizer: 第32回角膜カンファランス・第24回日本角膜移植学会
  • Place of Presentation: 東京
• [Presentation] 角膜上皮における幹細胞スイッチの可能性 (2008)
  • Author(s): 林康人, カオウィンストン, リウチャイアン, 大橋裕一
  • Organizer: 第32回角膜カンファランス・第24回日本角膜移植学会
  • Place of Presentation: 東京
• [Presentation] Cultivation of corneal epithelial cells on the collagen gel containing human dermal fibroblasts (2008)
  • Author(s): Atsushi Shiraihi. Takeshi Kobayashi, Yuko Kadota, Yuko Hara, Yuji Shirakata, Koji Hashimoto, Yuichi Ohashi
  • Organizer: 視覚と眼科学研究協議会
  • Place of Presentation: アメリカ・フロリダ
• [Remarks] ホームページ等