Establishment of the neurocristopathy treatment model and analysis of the differentiation mechanism of neural crest cells using mouse embryonic stem cells.

• Project/Area Number: 20592086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Gifu University
• Principal Investigator: MOTOHASHI Tsutomu Gifu University, 医学系研究科, 講師 (40334932)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経堤細胞 / ES細胞 / 神経堤症 / 転写因子Sox10 / 遺伝子改変マウス / セルソーター

Research Abstract

For the purpose of elucidating the mechanism of the neural crest cell differentiation, we generated the embryonic stem cells and mice, where the expression of Sox10 can be visualized with a fluorescent protein. The transplanted neural crest cells derived from the ES cells survived in the embryonic intestinal tract. We separated neural crest cells from the gene modified embryo and analyzed the gene expression by gene arrays. In addition, using the mice, we found that the multipotent neural crest-like cells were maintained in skin.

Research Products

• [Journal Article] Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages. (2011)
  • Author(s): Tsutomu Motohashi, Katsumasa Yamanaka, Kairi Chiba, Kentaro Miyajima, Hitomi Aoki, Tomohisa Hirobe, Takahiro Kunisada.
  • Journal Title: Developmental Dynamics. in press.
  • Peer Reviewed
• [Journal Article] Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages. (2011)
  • Author(s): Tsutomu Motohashi
  • Journal Title: Developmental Dynamics. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Functinally distinct melanocyte populations revealed by reconstitution of hair follicles in mice. (2010)
  • Author(s): Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Masataka Osawa, Takahiro Kunisada.
  • Journal Title: Pigment cell & melanoma Research. 24 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Unexpected multipotency of melanoblasts isolated from murine skin. (2009)
  • Author(s): Tsutomu Motohashi, Katsumasa Yamanaka, Kairi Chiba, Hitomi Aoki, Takahiro Kunisada.
  • Journal Title: STEM CELLS. 27 Pages: 888-897
  • Peer Reviewed
• [Journal Article] Unexpected multipotency of melanoblasts isolated from murine skin. (2009)
  • Author(s): Tsutomu Motohashi
  • Journal Title: STEM CELLS. 27 Pages: 888-897
  • Peer Reviewed
• [Journal Article] Differentiation of mouse hepatic progenitor cells induced by hepatocyte nuclear factor-4 and cell transplantation in mice with liver fibrosis. (2008)
  • Author(s): Atsushi Suetsugu, Masahito Nagaki, Hitomi Aoki, Tsutomu Motohashi, Takahiro Kunisada, Hisataka Moriwaki.
  • Journal Title: Transplantation. 86 Pages: 1178-1186
  • Peer Reviewed
• [Journal Article] Iris as a recipient tissue for pigment cells : Organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro. (2008)
  • Author(s): Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Huayue Chen, Takahiro Kunisada.
  • Journal Title: Developmental Dynamics. 237 Pages: 2394-2404
  • Peer Reviewed
• [Journal Article] Maintenance of Undifferentiated Mouse Embryonic Stem Cells in Suspentsion by the Serum- and Feeder-free Defined Culture Condition. (2008)
  • Author(s): Akiko Tsuji, Naoko Yoshimura, Hitomi Aoki, Alexei A.Sharov, Minoru S.H.Ko, Tsutomu Motohashi, Takahiro Kunisada.
  • Journal Title: Developmental Dynamics. 237 Pages: 2129-2138
  • Peer Reviewed
• [Journal Article] Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice. (2008)
  • Author(s): Atsushi Kawaguchi, Kairi Chiba, Yu Tanimura, Tsutomu Motohashi, Hitimi Aoki, Tomoko Takeda, Shinichi Hayashi, Katsuji Shimizu, Takahiro Kunisada.
  • Journal Title: development growth & differentiation. 50 Pages: 63-69
  • NAID: 10021095452
  • Peer Reviewed
• [Presentation] マウス内耳由来神経堤細胞の多分化能の解析 (2011)
  • Author(s): 本橋力
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] マウス色素芽細胞の持つ多分化能の解析 (2009)
  • Author(s): 本橋力
  • Organizer: 日本分子生物学会 第32回年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Unexpected multipotency of melanoblasts isolated from embryonic and neonatal skin. (2009)
  • Author(s): Tsutomu Motohashi
  • Organizer: 42th Annual Meeting for the Japanese Society of Developmental Biology
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-28
• [Presentation] マウス色素芽細胞の持つ多分化能の解析 (2009)
  • Author(s): 本橋 力
  • Organizer: 第8回再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] Analysis of multipotency of neural crest cells using mice that transcriotinal factor Sox10 is genetically modified. (2009)
  • Author(s): 本橋力
  • Organizer: 日本分子生物学会第32回年会
  • Place of Presentation: 横浜
• [Presentation] Unexpected multipotency of melanoblasts isolated from embryonic and neonatal skin. (2009)
  • Author(s): 本橋力
  • Organizer: 42th Annual Meeting for the Japanese Society of Developmental Biology
  • Place of Presentation: Niigata
• [Presentation] 「マウス色素芽細胞の持つ多分化能の解析」「各種の幹細胞」セッション (2009)
  • Author(s): 本橋力
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
• [Presentation] マウス色素芽細胞の持つ多分化能の解析 (2008)
  • Author(s): 本橋 力
  • Organizer: 第6回幹細胞シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-05-16
• [Presentation] Multipotent cell fates of melanocyte precursors isolated from embryonic and neonatal skin. (2008)
  • Author(s): Tsutomu Motohashi
  • Organizer: XXth International Pigment Cell Conference
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-09
• [Presentation] Concurrent Session 7-5, Multipotent cell fates of melanocyte precursors isolated from embryonic and neonatal skin. (2008)
  • Author(s): 本橋力
  • Organizer: XXth International Pigment Cell Conference(IPCC) & Vth International Melanoma Research Congress.
  • Place of Presentation: Royton Sapporo, Sapporo, Japan
• [Presentation] 「マウスES細胞由来の神経堤細胞の移植応用」、ポスター発表 (2008)
  • Author(s): 本橋力
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋
• [Remarks] ホームページ等
  • URL: http://www.med.gifu-u.ac.jp/saisei/index.htm
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/saisei/index.htm
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/saisei/index.htm
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/saisei/index.htm