The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization
| Project/Area Number |
20592089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
HAYASHIDA Makoto Jichi Medical University, 医学研究院, 助教 (70452761)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Tomoaki 九州大学, 医学研究院, 教授 (20197247)
MATSUURA Toshiharu 九州大学, 大学病院, 助教 (10532856)
高橋 由紀子 九州大学, 大学病院, 助教 (80380442)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 胆道閉鎖症 / 成因 / マイクロキメリズム |
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| Research Abstract |
Biliary atresia (BA) is a cholestatic disease of unknown etiology. It has recently been suggested that GVHD caused by microchimerism is an etiology in the development of autoimmune disease. Moreover, the liver is a frequent target organ of GVHD. The aim of this study is to identify the presence and extent of maternal microchimerism and to determine whether it play a role in the etiology of BA. The liver biopsy specimens of 6 male BA patients (BA group) and 6 males with other liver diseases (non-BA group) were assayed for X- and Y-chromosome using fluorescent in situ hybridization (FISH). The cells with two sex chromosomes in the nuclei were counted. Cells with one X- and one Y-chromosomes were considered to be host cells and those with two X-chromosome were considered to be of maternal origin. The frequency of cells with XX chromosomes per 1,000 host cells in the BA group and the non-BA group were 3.00±0.75 and 0.99±0.50, respectively. (P=0.005). The presence of female cells in the liver of male BA patients was significantly higher than in males with other liver disease. Maternal microchimerism is therefore suggested to contribute to the pathogenesis of BA.
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Report
(4 results)
Research Products
(37 results)
