| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Research Abstract |
Pressure ulcer occurs due to soft tissue irreversible injury by external forces such as perpendicular force, shear stress. The aim of this study demonstrates the effectof three factors which are perpendicular force, shear stress, ischemic reperfusion injury in mice by intravital microscopic techniques.
The cremaster muscle wistar rat model can observe the microcirculation in which blood flow is stopped by perpendicular force. Capillary vessel could not crush and occlude by 32mmHg
perpendicular force. We newly designed a compact capillaroscopy which was composed with a CCD video-probe equipped acontact-type objective lens and illuminator. In the present study, we evaluated usefulness of the instrument for a bed-side human capillaroscopy to observe the capillary flow in various dermal regions. The influences of tissue compression on the dermal capillary blood flow were also investigated to confirm the utility for clinical applications. Our capillaroscopy visualized the nutritional capillary blood flow in almost all parts of skin surface. Our observations showed that a level of vertical stress similar to arterial pressure was required to stop the capillary flow.
From these demonstrations the present CCD video-probe based capillaroscopy would be useful for clinical applications as a bed-side human capillaroscopy.
|
