Roles of cytoskeletal proteins in organic fibrosis and their application of therapy in fibrotic diseases

• Project/Area Number: 20592111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kinki University
• Principal Investigator: YOSHIDA Koji Kinki University, 医学部, 准教授 (60230736)
• Co-Investigator(Kenkyū-buntansha): SAITO Akio 近畿大学, 医学部, 講師 (40153788) ; HAGIHARA Satoru 近畿大学, 医学部, 講師 (40460852)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 線維化 / 線維芽細胞 / transgelin / integrin α11 / CIB1 / インテグリン / integrin / fibronectin / CTGF / 創傷治癒 / two-hybrid / shRNA / TGF-β

Research Abstract

Activation of myofibroblasts was inhibited when the expression of transgelin was knocked down. When human lung fibroblasts were stimulated by TGF-β, the integrin α11 (ITGA11) expression was markedly increased. Calcium and integrin binding protein 1 (CIB1) bound to the intracellular domain of ITGA11. The expression of CIB1 increased in human lung fibroblasts treated with TGF-β and in bleomycin-treated mice pulmonary tissues. These results suggest that CIB1 may be involved in the fibrosis.

Research Products

• [Journal Article] Connective Tissue Growth Factor Cooperates with Fibronectin in Enhancing Attachment and Migration of Corneal Epithelial Cells (2010)
  • Author(s): Sugioka K, Yoshida K, Kodama A, Mishima H, Abe K, Munakata H, Shimomura Y.
  • Journal Title: Tohoku J Exp Med. 222 Pages: 45-50
  • NAID: 10027085615
  • Peer Reviewed
• [Journal Article] Transforming growth factor-β upregulates the expression of integrin and related proteins in MRC-5 human myofibroblasts (2010)
  • Author(s): Honda E, Yoshida K, Munakata H.
  • Journal Title: Tohoku J Exp Med. 220 Pages: 319-327
  • NAID: 10027084026
  • Peer Reviewed
• [Journal Article] Transforming growth factor-beta upregulates the expression of integrin and related proteins in MRC-5 human myofibroblasts (2010)
  • Author(s): Honda Eiko
  • Journal Title: Tohoku J.Exp.Med. Volume: 220 Pages: 319-327
  • Peer Reviewed
• [Journal Article] Connective tissue growth factor cooperates with fibronectin in enhancing attachment and migration of corneal epithelial cells (2010)
  • Author(s): Sugioka Koji
  • Journal Title: Tohoku J.Exp.Med. Volume: 222 Pages: 45-50
  • Peer Reviewed
• [Presentation] インテグリンalpha11とcalcium and integrin binding protein 1の相互作用 (2010)
  • Author(s): 吉田浩二
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] インテグリンalpha11とcalcium and integrin binding protein 1の相互作用 (2010)
  • Author(s): 吉田浩二
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸ポートアイラン(神戸市)
  • Year and Date: 2010-12-10
• [Presentation] 角膜上皮細胞の伸長、接着に対するconnective tissue growth factorとfibronectinの相互作用 (2010)
  • Author(s): 杉岡孝二
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] 肺線維化におけるheat shock protein 27の役割 (2010)
  • Author(s): 朴雅美
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] 角膜上皮細胞の伸長、接着に対するconnective tissue growth factor とfibronectinの相互作用 (2010)
  • Author(s): 杉岡孝二
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] 肺線維化におけるheat shock protein 27の役割 (2010)
  • Author(s): 朴雅美
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] 角膜上皮細胞の接着、伸長に対するCTGFとフィプロネクチンの相互作用 (2009)
  • Author(s): 杉岡孝二
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2009-04-16
• [Presentation] TGF-βによる筋線維芽細胞(MRC-5)の活性化に伴う発現変化 (2008)
  • Author(s): 本田映子
  • Organizer: 第31回日本分子生物学会合同年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-12
• [Presentation] transgelinノックダウンが肺線維芽細胞MRC-5に及ぼす効果 (2008)
  • Author(s): 吉田浩二
  • Organizer: 第31回日本分子生物学会合同年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] transgelin遺伝子ノックダウンが肺線維芽細胞MRC-5に及ぼす効果 (2008)
  • Author(s): 吉田浩二
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10