| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
We verified the effectiveness ofβ3 integrin-recombinant cultured epithelium by animal experiments. Human kerainocytes were transduced withβ3 integrin subunit cDNA by a retrovirus-mediated transduction method.β3 positive cells were enriched by cell sorting (89% positive), and were cultured on fibrin gels for 5 days to make the cultured epithelium.The cultured epithelia were transplanted to the backs of nude mice on which full-thickness wounds were topically treated with Mitomycin C to make a retarded wound healing model. Cultured epithelia made ofβ-galactosidase cDNA-transduced keratinocytes were used as a control. Histological examination was done with the samples taken at three time points (1, 2, 3 weeks).Cultured epithelia made ofβ3-recombinant keratinocytes increased its take-rate in a slight to moderate degree at all time points.Further experiments should be done to determine the effectiveness of the cultured epithelium in vivo.
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