Verification for the effectiveness of β3 integrin-recombinant cultured epithelium by animal experiments
| Project/Area Number |
20592112
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
KUBO Miyoko Kawasaki Medical School, 医学部, 講師 (00098609)
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| Co-Investigator(Kenkyū-buntansha) |
MORIGUCHI Takahiko 川崎医療福祉大学, 医療技術学部, 教授 (10111816)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 再生医学 / 培養表皮 / 移植・再生医療 / 動物実験 |
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| Research Abstract |
We verified the effectiveness ofβ3 integrin-recombinant cultured epithelium by animal experiments. Human kerainocytes were transduced withβ3 integrin subunit cDNA by a retrovirus-mediated transduction method.β3 positive cells were enriched by cell sorting (89% positive), and were cultured on fibrin gels for 5 days to make the cultured epithelium.The cultured epithelia were transplanted to the backs of nude mice on which full-thickness wounds were topically treated with Mitomycin C to make a retarded wound healing model. Cultured epithelia made ofβ-galactosidase cDNA-transduced keratinocytes were used as a control. Histological examination was done with the samples taken at three time points (1, 2, 3 weeks).Cultured epithelia made ofβ3-recombinant keratinocytes increased its take-rate in a slight to moderate degree at all time points.Further experiments should be done to determine the effectiveness of the cultured epithelium in vivo.
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Report
(4 results)
Research Products
(15 results)
