Expression of a lipid-metabolizing enzyme, diacylglycerol kinase, after brain damage

• Project/Area Number: 20592116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Yamagata University
• Principal Investigator: KAWAMAE Kaneyuki 山形大学, 医学部, 教授 (70254026)
• Co-Investigator(Kenkyū-buntansha): GOTO Kaoru 山形大学, 医学部, 教授 (30234975) ; ISEKI Ken 山形大学, 医学部, 准教授 (70332921)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 脳損傷 / 脂質代謝酵素 / ミクログリア / グリア細胞 / リモデリング / KOマウス / 脂質代謝 / 神経細胞 / 核

Research Abstract

Diacyglycerol kinase (DGK) plays an important role in the intracellular signal transduction through the metabolism of a second messenger diacylglycerol. One of the DGK isozymes, DGKζ was originally shown to be expressed in neurons under physiological conditions. However, we found that under pathological conditions, a cryoinjury brain model, DGKζ-immunoreactivity is detected in activated microglia that phagocytose the debris after brain injury. These results suggest that DGK. participates in the remodeling of the brain after injury.

Research Products

• [Journal Article] NAP Interaction of nucleosome assembly proteins abolishes nuclear localization of DGKζ by attenuating its association with importins (2011)
  • Author(s): Okada M, Hozumi Y, Ichimura T, Tanaka T, Hasegawa H, Yamamoto M, Takahashi N, Iseki K, Yagisawa H, Shinkawa T, Isobe T, Goto K
  • Journal Title: Exp Cell Res Volume: 317 Pages: 2853-63
  • Peer Reviewed
• [Journal Article] Excitotoxicity by kainate-induced seizure causes diacylglycerol kinase ζ to shuttle from the nucleus to the cytoplasm in hippocampal neurons (2011)
  • Author(s): Saino-S S, Hozumi Y, Goto K
  • Journal Title: Neurosci Lett Volume: 494 Pages: 185-9
  • Peer Reviewed
• [Journal Article] Altered expression pattern of testican-1 mRNA after brain injury (2011)
  • Author(s): Iseki K, Hagino H, Zhang Y, Mori T, Sato N, Yokoya S, Hozumi Y, Goto K, Tase C
  • Journal Title: Biomed Res Volume: 32 Pages: 373-8
  • NAID: 130004470790
  • Peer Reviewed
• [Journal Article] 集中治療室における危機管理体制の現状-全国調査 (2011)
  • Author(s): 川前金幸, 鮎川勝彦, 内野博之, 中敏夫, 西田修, 成松英智, 池田寿昭
  • Journal Title: 日本集中治療医学会 Volume: 18 Pages: 116-25
  • Peer Reviewed
• [Journal Article] The Tohoku ALI Study Group. A prospective cohort study of ALI/ARDS in the Tohoku district of Japan (second report) (2010)
  • Author(s): Endo S, Shibata S, Sato N, Hashiba E, Tajjimi K, Saito K, Kawamae K, Nakane M, Murakawa M
  • Journal Title: J Anesth Volume: 24 Pages: 351-358
  • Peer Reviewed
• [Journal Article] Identification of a functional nuclear export sequence in diacylglycerol kinase (2010)
  • Author(s): Evangelisti C, Gaboardi GC, Billi AM, Ognibene A, Goto K, Tazzari PL, McCubrey JA, Martelli AM
  • Journal Title: Cell Cycle Volume: 9 Pages: 1-6
  • Peer Reviewed
• [Journal Article] 手術中に覚醒を必要とする脳神経外科手術(awake craniotomy)の麻酔管理におけるレミフェンタニルの有効性 (2010)
  • Author(s): 岡田真行, 高田康輔, 川前金幸
  • Journal Title: 麻酔 Volume: 59 Pages: 75-81
  • Peer Reviewed
• [Journal Article] アナフィラキシーショックの治療にβ遮断薬が影響を及ぼした心肺停止に陥った1症例 (2010)
  • Author(s): 永野達也, 伊関憲, 仁木敬夫, 杉浦明日美, 二藤部丈司, 川前金幸
  • Journal Title: 日本集中治療医学会雑誌 Volume: 17 Pages: 207-10
  • Peer Reviewed
• [Journal Article] Signaling Cascade of Diacylglycerol Kinase beta in the Pituitary Intermediate Lobe : Dopamine D2 Receptor/Phospholipase C-beta4/Diacylglycerol Kinase-beta/Protein Kinase C-alpha (2010)
  • Author(s): Hozumi Y, et al.
  • Journal Title: J Histochem Cytochem Volume: 58 Pages: 119-129
  • Peer Reviewed
• [Journal Article] Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta (2010)
  • Author(s): Evangelisti C, et al.
  • Journal Title: Cell Cycle Volume: 9 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Brain traume induces ex-pression of diacylglycerol kinase ζ in microglia (2009)
  • Author(s): Nakano T, Iseki K, Hozumi Y, Kawamae K, Wakabayashi I, Goto K
  • Journal Title: Neurosci Lett Volume: 46 Pages: 110-115
  • Peer Reviewed
• [Journal Article] TIS21/BTG2/PC3 and cyclin D1 are key determinants of nuclear diacylglycerol kinase-ζ-dependent cell cycle arrest (2009)
  • Author(s): Evangelisti C, Astolfi A, Gaboardi GC, Tazzari P, Pession A, Goto K, Martelli AM
  • Journal Title: Cellular Signalling Volume: 21 Pages: 801-9
  • Peer Reviewed
• [Journal Article] 嫌酒者によるメタノール中毒の1例 (2009)
  • Author(s): 伊関憲, 永野達也, 杉浦明日美, 金井雅代, 仁木敬夫, 堀寧, 川前金幸
  • Journal Title: 中毒研究 Volume: 22(3) Pages: 238-239
  • Peer Reviewed
• [Journal Article] Brain trauma induces expression of diacylglycerol kinase zeta in microglia (2009)
  • Author(s): Nakano T, Iseki K, Hozumi Y, Kawamae K, Wakabayashi I, Goto K.
  • Journal Title: Neurosci Lett. 461 Pages: 110-115
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase beta promotes dendritic outgrowth and spine maturation in developing hippocampal neurons (2009)
  • Author(s): Hozumi, et al.
  • Journal Title: BMC Neurosci. 10 Pages: 99-99
  • Peer Reviewed
• [Journal Article] Localization of diacylglycerol kinase epsilon on stress fibers in vaseular smooth muscle cells (2009)
  • Author(s): Nakano, et al.
  • Journal Title: Cell Tissue Res. 337 Pages: 167-175
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase ζ is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells (2008)
  • Author(s): Hasegawa H, Nakano T, Hozumi Y, Takagi M, Ogino T, Okada M, Iseki K, Kondo H, Watanabe M, Martelli AM, Goto K
  • Journal Title: J Cell Biochem Volume: 105 Pages: 756-65
  • Peer Reviewed
• [Journal Article] 剖検により判明した心サルコイドーシスの1例 (2008)
  • Author(s): 大瀧恵, 伊関憲, 池谷龍一, 奥山志野, 永野達也, 山川光徳, 川前金幸
  • Journal Title: 日本救急医学会雑誌 Volume: 19 Pages: 229-234
  • NAID: 10024372179
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase beta accumulates on the perisynaptic site of medium spiny neurons in the striatum (2008)
  • Author(s): Hozumi, et.al.
  • Journal Title: Eur J Neurosci 28 Pages: 2409-2422
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase zeta is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells (2008)
  • Author(s): Hasegawa, et.al.
  • Journal Title: J Cell Biochem 105 Pages: 756-765
  • Peer Reviewed
• [Journal Article] Lipid messenger, diacylglycerol, and its regulator, diacylglycerol kinase, in cells, organs, and animals : history and perspective (2008)
  • Author(s): Goto et.al.
  • Journal Title: Tohoku J Exp Med 214 Pages: 199-212
  • NAID: 10024167082
  • Peer Reviewed
• [Presentation] ジアシルグリセロールキナーゼの細胞内局在の制御機構 (2010)
  • Author(s): 後藤薫
  • Organizer: 第5回DGK研究会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-11
• [Presentation] Inspiratory Trigger and Expiratory Termination Sensitivity of New Anesthesia Ventilators (2010)
  • Author(s): Nakane M, Kurita S, Takahashi N, Yoshioka J, Oda S, Kawamae K
  • Organizer: American Society of Anesthesiologists annual congress 2010
  • Place of Presentation: San Diego
• [Presentation] 日本集中治療医学会危機管理委員会:各施設の集中治療部における危機管理体制の現状 (2010)
  • Author(s): 川前金幸, 鮎川勝彦, 内野博之, 中敏夫, 西田修, 成松英智, 池田寿昭
  • Organizer: 第37回日本集中治療医学会
  • Place of Presentation: 広島
• [Presentation] Nuclear assembly proteinはDGKζの細胞内局在を制御する (2010)
  • Author(s): 岡田雅司, 後藤薫
  • Organizer: 第115回日本解剖学会総会
  • Place of Presentation: 盛岡
• [Presentation] DNA損傷におけるジアシルグリセロールキナーゼζとp53の役割 (2010)
  • Author(s): 田中俊昭, 後藤薫
  • Organizer: 第115回日本解剖学会総会
  • Place of Presentation: 盛岡
• [Presentation] 無酸素無グルコース負荷(OGD)によるζ型ジアシルグリセロールキナーゼの細胞内局在の変化:海馬スライスを用いた検討 (2010)
  • Author(s): 鈴木祐輔, 後藤薫
  • Organizer: 第115回日本解剖学会総会
  • Place of Presentation: 盛岡
• [Presentation] 細胞質局在型ジアシルグリセロールキナーゼξは細胞死を抑制する (2010)
  • Author(s): 田中俊昭, ら
  • Organizer: 第56日本解剖学会・東北北海道地方会
  • Place of Presentation: 旭川
• [Presentation] Cytoplasmic lipid kinase suppresses p53 stability (2009)
  • Author(s): Tanaka T, Okada M, Goto K
  • Organizer: 39th Annual Meeting of Neuroscience
  • Place of Presentation: Chicago, USA
• [Presentation] Nucleocytoplasmic translocation of diacylglycerol kinase zeta under oxygen and glucose deprivation in rat hippocampal slices (2009)
  • Author(s): Suzuki Y, Yamazaki Y, Kaneko K, Fujii S, Iseki K, Goto K
  • Organizer: 39th Annual Meeting of Neuroscience
  • Place of Presentation: Chicago, USA
• [Presentation] Upregulation of agrin in the reactive astrocytes after brain injury (2009)
  • Author(s): Iseki K, Goto K
  • Organizer: 9th European Meeting on Glial Cells in Health and Disease
  • Place of Presentation: Paris, France
• [Presentation] ジアシルグリセロールキナーゼの血管内皮細胞における発現解析 (2009)
  • Author(s): 中野知之, 若林一郎, 後藤薫
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
• [Presentation] Nucleocytoplasmic translocation of diacylglycerol kinase zeta under oxygen and glucose deprivation in rat hippocampal slices (2009)
  • Author(s): Suzuki Y, Yamazaki Y, Kaneko K, Fujii S, Iseki K, Goto K.
  • Organizer: 39th Annual Meeting of Neuroscience
  • Place of Presentation: Chicago
• [Presentation] 細胞質局在型ジアシルグリセロールキナーゼζはp53の発現を抑制する (2009)
  • Author(s): 田中俊昭、岡田雅司、後藤薫
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山
• [Presentation] Expression and subcellular localization of diacylglycerol kinase isozyme in rat aortic endothelial cells (2008)
  • Author(s): Nakano T, Goto K, Wakabayashi I
  • Organizer: 48th American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
• [Presentation] Diacylglycerol kinase β accumulates on the perisynaptic site of medium spiny neurons in the striatum (2008)
  • Author(s): Hozumi Y, Iseki K, Kawamae K, Goto K
  • Organizer: 38th Annual Meeting of Neuroscience
  • Place of Presentation: Washington DC, USA
• [Presentation] Diacylglycerol kinase b accumulates on the perisynaptic site of medium spiny neurons in the striatum (2008)
  • Author(s): Yasukazu Hozumi, Ken Iseki, Kaneyuki Kawamae, Kaoru Goto
  • Organizer: 38th Annual Meeting of Neuroscience
  • Place of Presentation: Washington D. C.