| Project/Area Number |
20592120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Okayama University |
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Principal Investigator |
KATAYAMA Hiroshi Okayama University, 岡山大学病院, 准教授 (90161067)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Kiyoshi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40108171)
SHIMIZU Hiroko 岡山大学, 医学部, 客員研究員 (80423284)
TAKAHASHI Toru 岡山大学, 大学病院, 准教授 (40252952)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 消化管傷害 / グルタミン / ストレス蛋白 / 炎症 / アポトーシス / 出血性ショック / 消化管障害 / ヘムオキシゲナーゼ-1 / 自然免疫 / SIRS / MODS / HIF-1alpha |
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| Research Abstract |
Hemorrhagic shock (HS) is an oxidative stress which causes intestinal tissue injury. Heme oxygenase (HO)-1 is induced by oxidative stress, and is thought to play an important role in the protection of tissues from oxidative injury. We previously reported the ileum to be the most susceptible to HS-induced tissue injury site in the intestine, because HO-1 induction is the lowest at this site. We also previously demonstrated that glutamine significantly induced HO-1 in the lower intestinal tract. In the present study, we investigated whether glutamine pretreatment improves HS-induced intestinal tissue injury in the ileum by HO-1 induction. Treatment of rats with glutamine markedly induced functional HO-1 protein in mucosal epithelial cells in the ileum. Glutamine treatment before HS (mean arterial blood pressure of 30mmHg for 60min) significantly ameliorated HS-induced mucosal inflammation and apoptotic cell death in the ileum, In contrast, treatment with a specific inhibitor of HO activity, abolished the beneficial effect of glutamine pretreatment. These findings indicate that glutamine pretreatment significantly ameliorated tissue injury in the ileum after HS by inducing HO-1.
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