Role of C/EBPβ, a transcription factor in neutrophils, in the pathogenesis of septic acute lung injury

• Project/Area Number: 20592125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SHIME Nobuaki Kyoto Prefectural University of Medicine, 医学研究科, 講師 (00260795)
• Co-Investigator(Kenkyū-buntansha): HIRAI Hideyo 京都大学, 医学研究科, 助教 (50315933)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 集中治療医学 / 好中球 / 急性肺損傷 / 敗血症 / 転写因子 / C/EBPβ / C / EBPβ / 造血過程 / フローサイトメーター

Research Abstract

The purpose of this study is to obtain basic data for maintaining proper body weight by controlling obesity following childbirth. The levels of autonomic nerve activity and body composition were measured in pregnant and puerperal women by analyzing the power spectra of heat rate fluctuations. Results 1) Parasympathetic nerve activity decreased while sympathetic nerve activity increased in the third trimester as compared with the second trimester. 2) Increased body fat percentage, decreased muscle volume and increased autonomic nerve activity were confirmed following childbirth. The results of this study can be applied to the development of a proper weight management program for use after childbirth by monitoring BMI and body composition starting one year after childbirth.

Research Products

• [Journal Article] Eradication of the commensal intestinal microflora by oral antimicrobials interferes with the host response to lipopolysaccharide. (2010)
  • Author(s): Umenai T, Hirai H, Shime N, Nakaya T, Asahara T, Nomoto K, Kita M, Tanaka Y, Imanishi J.
  • Journal Title: Eur J Clin Microbiol Infect Dis 29(6) Pages: 633-641
  • Peer Reviewed
• [Journal Article] The importance of a judicious and early empiric choice of antimicrobial for methicillin-resistant Staphylococcus aureus bacteraemia. (2010)
  • Author(s): Shime N, Kosaka T, Fujita N.
  • Journal Title: Eur J Clin Microbiol Infect Dis 29(12) Pages: 1475-1479
  • Peer Reviewed
• [Journal Article] Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences. (2010)
  • Author(s): Kamio N, Hirai H, Ashihara E, Tenen DG, Maekawa T, Imanishi J
  • Journal Title: Biochem Biophys Res Commun 393 Pages: 498-503
  • NAID: 120002426875
  • Peer Reviewed
• [Journal Article] The importance of a judicious and early empiric choice of antimicrobial for methicillin-resistant Staphylococcus aureus bacteraemia. (2010)
  • Author(s): Shime N, et al.
  • Journal Title: Eur J Clin Microbiol Infect Dis Volume: 29 Pages: 1475-9
  • Peer Reviewed
• [Journal Article] Eradication of the commensal intestinal microflora by oral antimicrobials interferes with the host response to lipopolysaccharide. (2010)
  • Author(s): Umenai T, Hirai H, Shime N, et al.
  • Journal Title: Eur J Clin Microbiol Infect Dis Volume: 29 Pages: 633-41
  • Peer Reviewed
• [Journal Article] Prediction of pediatric endotracheal tube size by ultrasonography. (2010)
  • Author(s): Shibasaki M, Nakajima Y, Ishii S, Shimizu F, Shime N, et al.
  • Journal Title: Anesthesiology Volume: 113 Pages: 819-24
  • Peer Reviewed
• [Journal Article] Eradication of the commensal intestinal microflora by otalantimicrobials interferes with the host response to lipopolysaccharide (2010)
  • Author(s): Umenai T, Shime N, Hirai H, et al.
  • Journal Title: Eur J Clin Microbiol Infect Dis (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Pharmacokinetics and tolerance of linezolid for meticillin-resistant Staphylococcus aureus mediastinitis in paediatric patients. (2009)
  • Author(s): Kosaka T, Kokufu T, Shime N, Sugioka N, Kato R, Hamaoka K, Fujita N.
  • Journal Title: Int J Antimicrob Agents 32(12) Pages: 368-370
  • Peer Reviewed
• [Journal Article] Hyperventilation versus standard ventilation for infants in postoperative care for congenital heart defects with pulmonary hypertension. (2009)
  • Author(s): Umenai T, Shime N, Kato Y, Hashimoto S.
  • Journal Title: J Anesth 23(81) Pages: 80-86
  • NAID: 10025381685
  • Peer Reviewed
• [Journal Article] Clinical and hemodynamic factors associated with the outcome of early extubation attempts after right heart bypass surgery. (2009)
  • Author(s): Kurihara Y, Shime N, Miyazaki T, Kato Y, Hashimoto S.
  • Journal Title: Interact Cardiovasc Thorac Surg 8(6) Pages: 624-628
  • Peer Reviewed
• [Journal Article] Disseminated Aspergillosis Following Resolution of Pneumocystis Pneumonia with Sustained Elevation of Beta-Glucan in an Intensive Care Unit : a Case Report. (2009)
  • Author(s): Saito T, Shime N, Itoh K, Fujita N, Saito Y, Shinozaki M, Shibuya K, Makimura K, Hashimoto S.
  • Journal Title: Infection 37(6) Pages: 547-550
  • Peer Reviewed
• [Journal Article] Identification of a myeloid committed progenitor as the cancer initiating cell in acute promyelocytic leukemia. (2009)
  • Author(s): Guibal FC, Alberich-Jorda M, Hirai H, Ebralidze A, Levantini E, Di Ruscio A, Zhang P, Santana-Lemos BA, Neuberg D, Wagers AJ, Rego EM, Tenen DG
  • Journal Title: Blood 114(27) Pages: 5415-5425
  • Peer Reviewed
• [Journal Article] Toll-like receptors (TLRs) are expressed by myeloid leukaemia cell lines, but fail to trigger differentiation in response to the respective TLR ligands. (2009)
  • Author(s): Okamoto M, Hirai H, Taniguchi K, Shimura K, Inaba T, Shimazaki C, Taniwaki M, Imanishi J
  • Journal Title: Br J Haematol 147(4) Pages: 585-587
  • Peer Reviewed
• [Journal Article] C/EBPファミリー転写因子による好中球産生の制御機構 (2009)
  • Author(s): 平位秀世
  • Journal Title: 医学のあゆみTOPICS 23(12) Pages: 1199-1200
• [Journal Article] Pharmacokinetics and tolerance of linezolid for meticillin-resistant Staphylococcus aureus mediastinitis in paediatric patients. (2009)
  • Author(s): Kosaka T, Kokufu T, Shime N, et al.
  • Journal Title: Int J Antimicrob Agents 32 Pages: 368-370
  • Peer Reviewed
• [Journal Article] Hyperventilation versus standard ventilation for infants in postoperative care for congenital heart defects with pulmonary hypertension. (2009)
  • Author(s): Umenai T, Shime N, et al.
  • Journal Title: J Anesth 23 Pages: 80-86
  • NAID: 10025381685
  • Peer Reviewed
• [Journal Article] Fas ligand released by activated monocytes causes apoptosis of lung epithelial cells in human acute lung injury model in vitro. (2008)
  • Author(s): Mizuta M, Nakajima H, Mizuta N, Kitamura Y, Nakajima Y, Hashimoto S, Matsuyama H, Shime N, Amaya F, Koh H, Ishizaka A, Magae J, Tanuma SI, Hashimoto S.
  • Journal Title: Biol Pharm Bull 31 Pages: 386-390
  • NAID: 110006623061
  • Peer Reviewed
• [Journal Article] Current problems in the diagnosis and treatment of hospital-acquired methicillin-resistant Staphylococcus aureus pneumonia. (2008)
  • Author(s): Sakaguchi M, Shime N, Fujitaitae, FujitaaFujiki S, Hashimoto S.
  • Journal Title: J Anesth. 22(2) Pages: 125-130
  • NAID: 10024128947
  • Peer Reviewed
• [Journal Article] Neutrophil elastase activity in acute lung injury and respiratory distress syndrome. (2008)
  • Author(s): Hashimoto S, Okayama Y, Shime N, Kimura A, Funakoshi Y, Kawabata K, Ishizaka A, Amaya F.
  • Journal Title: Respirology 13(4) Pages: 581-584
  • Peer Reviewed
• [Journal Article] Acute lung inflammation and ventilator-induced lung injury caused by ATP via the P2Y receptors : an experimental study. (2008)
  • Author(s): Matsuyama H, Amaya F, Hashimoto S, Ueno H, Beppu S, Mizuta M, Shime N, Ishizaka A, Hashimoto S.
  • Journal Title: Respir Res 9 Pages: 79-79
  • Peer Reviewed
• [Journal Article] Neutrophil elastase activity in acute lung injury and respiratory distress syndrome (2008)
  • Author(s): Hashimoto S, Okayama Y, Shime N, et al.
  • Journal Title: Respirology 13 Pages: 581-4
  • Peer Reviewed
• [Journal Article] Current problems in the diagnosis and treatment of hospital-acquired methicillin-resistant Staphylococcus aureus pneumonia (2008)
  • Author(s): Sakaguchi M, Shime N, et al.
  • Journal Title: J anesth 22 Pages: 125-30
  • NAID: 10024128947
  • Peer Reviewed
• [Journal Article] Acute lung inflammation and ventilator-induced lung injury caused by ATP via the P2Y receptors : an experimental study. (2008)
  • Author(s): Matsuyama H, Amaya F, Shime N, et al
  • Journal Title: Respir Res 9 Pages: 79-79
  • Peer Reviewed
• [Presentation] Candidemia-Induced Emergency Granulopoiesis Consist of Successive Dual Waves Triggered by the Shift from C/EBPalpha Dependency to C/EBPbeta Dependency. (2010)
  • Author(s): 佐竹早紀子, 平位秀世, 志馬伸朗, 長尾里奈, 田中瑠璃子, 八尾尚幸, 林嘉宏, 稲葉亨, 藤田直久, 今西二郎, 前川平
  • Organizer: 52st ASH ANNUAL MEETING AND EXPOSITION
  • Place of Presentation: ORLAND
  • Year and Date: 2010-12-06
• [Presentation] Candidemia-Induced Emergency Granulopoiesis Consist of Successive Dual Waves Triggered by the Shift from C/EBPalpha Dependency to C/EB Pbeta Dependency (2010)
  • Author(s): Satake S, Hirai H, Shime N, et al.
  • Organizer: 52^<st> ASH ANNUAL MEETING AND EXPOSITION
  • Place of Presentation: ORLAND, FL, USA.
  • Year and Date: 2010-12-06
• [Presentation] 敗血症による2相性"緊急"好中球造血のフローサイトメトリーによる解析 (2010)
  • Author(s): 佐竹早紀子, 平位秀世, 志馬伸朗, 長尾里奈, 田中瑠璃子, 八尾尚幸, 林嘉宏, 武内美紀, 芦原英司, 稲葉亨, 藤田直久,今西二郎, 前川平
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] 「骨髄系細胞の分化と機能制御」「緊急」顆粒球造血時のC/EBPβの制御におけるCREBファミリー転写因子 (2010)
  • Author(s): Hirai H, Kamio N, Matsusue A, Ogino S, Kimura N, Satake S, Tanaka R, Nagao R, Yao H, Hayashi Y, Takeuchi M, Ashihara E, Huang G, Tenen DG, Imanishi J, Maekawa T
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] 敗血症時の好中球造血における転写因子の機能解析 (2010)
  • Author(s): 佐竹早紀子, 平位秀世, 志馬伸朗, 芦原英司, 稲葉亨, 藤田直久, 今西二郎, 前川平
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-06
• [Presentation] Performance of evidence-based preventive measures for ventilator-associated pneumonia in Japanese healthcare settings. (2010)
  • Author(s): Shime N, Satake S, Hosokawa K, Hashimoto S
  • Organizer: The 10th. joint scientific congress of the Korean society of critical care medicine and Japanese society of intensive care medicine.
  • Place of Presentation: Hiroshima
  • Year and Date: 2010-03-06
• [Presentation] CREB Is Involved in the Regulation of C/EBPβ During 'emergency' Granulopoiesis. (2009)
  • Author(s): Hirai H, Kamio N, Matsusue A, Ogino S, Kimura N, Satake S, Ashihara E, Maekawa T, Tenen DG, Imanishi J
  • Organizer: The American Society of Hematology 51st Annual Meeting and Exposition
  • Place of Presentation: New Orleans
  • Year and Date: 2009-12-05
• [Presentation] Involvement of CREB in the regulation of "emergency" granulopoiesis through upregulation of C/EBP Kyoto University Global COE Center for Frontier Medicine (2009)
  • Author(s): 平位秀世
  • Organizer: International Symposium/Retreat2009.
  • Place of Presentation: Hyogo
  • Year and Date: 2009-11-07
• [Presentation] Sweep of the intestinal microflora induced by oral administration of antibiotics impairs host response to LPS through down-regulation of the surface Toll-like receptors expressions on the peritoneal macrophages. (2009)
  • Author(s): Umenai T, Hirai H, Nakaya T, Nakaya T, Tanaka Y, Imanishi J
  • Organizer: The 49th. Interscience Conference on Antimicrobial Agents and Chemotherapy.
  • Place of Presentation: San Francisco
  • Year and Date: 2009-09-12
• [Presentation] Ventilator-associated pneumonia. (2009)
  • Author(s): 志馬伸朗
  • Organizer: The 9th. joint scientific congress of the Korean society of critical care medicine and Japanese society of intensive care medicine
  • Place of Presentation: Seoul
  • Year and Date: 2009-04-25
• [Presentation] フローサイトメーターを用いた敗血症における顆粒球造血過程の検討 (2009)
  • Author(s): 藤木早紀子, 平位秀世, 志馬伸朗, 稲葉亨, 藤田直久, 今西二郎
  • Organizer: 第83回日本感染症学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-24
• [Presentation] 顆粒球造血過程をとらえるためのフローサイト新染色法を用いた敗血症病態の検討 (2009)
  • Author(s): 佐竹早紀子、平位秀世、志馬伸朗, ら
  • Organizer: 日本感染症学会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-24
• [Book] 人工呼吸器関連肺炎のすべて (2010)
  • Author(s): 志馬伸朗(編著)
  • Publisher: 東京:南江堂
• [Book] 人工呼吸器関連肺炎のすべて (2010)
  • Author(s): 志馬伸朗
  • Total Pages: 304
  • Publisher: 南江堂
• [Book] 抗菌薬のかしこい使い方 (2009)
  • Author(s): 志馬伸朗(分担執筆)
  • Total Pages: 378
  • Publisher: 南江堂
• [Book] 小児救急医療(小児科臨床ピクシス1) (2008)
  • Author(s): 志馬伸朗(分担)
  • Total Pages: 240
  • Publisher: 中山書店