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Analysis of biomarker for septic shock

Research Project

Project/Area Number 20592132
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Emergency medicine
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

YAMASHITA Uki  University of Occupational and Environmental Health, Japan, 医学部, 名誉教授 (00028680)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Yasuhiro  産業医科大学, 医学部, 准教授 (10309958)
SUGIURA Tsutomu  産業医科大学, 産業医学研究支援施設, 准教授 (40131924)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsSeptic shock / プロスタグランジン / PG合成酵素ノックアウトマウス / Septic Shock / マクロファージ / 免疫応答 / Th1, Th2
Research Abstract

We analyzed the role of prostaglandin (PG) in acute inflammatory reactions and the following immune responses using PG synthetase knockout (KO) mice. The production of cytokine from LPS-stimulated macrophages was decreased in PG-KO mice in comparison with wild type (WT) mice. On the other hand, the serum cytokine level from LPS-treated mice was higher in PG-KO mice than WT mice. In the LPS-challenged mice, serum GOT level was decreased in PG-KO mice. However, GPT level was equal between PG-KO and WT mice. The function of helper T cells after immunization with protein antigens in PG-KO mice was different from that of WT mice. Taken together, these results suggest that PG played an important role for the regulation of inflammatory and immune responses such as cytokine productions.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (43 results)

All 2011 2010 2009 2008

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (22 results)

  • [Journal Article] Apoptosis induction through proteasome inhibitory activity of cucurbitacin D in human T-cell leukemia.2011

    • Author(s)
      Ding N., Yamashita U., Matsuoka H., Sugiura T., Tsukada J., Noguchi J., Yoshida Y.
    • Journal Title

      Cancer (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stimulation of IFN-gamma production by garlic lectin in mouse spleen cells : Involvement of IL-12 via activation of p38 MAPK and ERK in macrophages.2011

    • Author(s)
      Dong Q., Sugiura T., Toyohira Y., Yoshida Y., Yanagihara N., Karasaki Y.
    • Journal Title

      Phytomedicine 18

      Pages: 309-316

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Apoptosis induction through proteasome inhibitory activity of cucurbitacin D inhuman T-cell leukemia.2011

    • Author(s)
      Ding, N., Yamashita, U., Sugiura, T., Yoshida Y., et al.
    • Journal Title

      Cancer

      Volume: In press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimulation of IFN-gamma production by garlic lectin in mouse spleen cells : Involvement of IL-12 via activation of p38 MAPK and ERK in macrophages.2011

    • Author(s)
      Dong, Q., Sugiura, T., Yoshida Y., et al.
    • Journal Title

      Phytomedicine

      Volume: 18 Pages: 309-316

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thymocytes are activated by toluene inhalation through the transcription factors NF-κB, STAT5 and NF-AT.2010

    • Author(s)
      Liu JQ., Yoshida Y., Kunugita N., Noguchi J., Sugiura T., Ding N., Arashidani K., Fujimaki H., Yamashita U.
    • Journal Title

      Journal of Applied Toxicology 30

      Pages: 656-660

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thymocytes are activated by toluene inhalation through the transcription factors NF-κ B, STAT5 and NF-AT.2010

    • Author(s)
      Liu, JQ., Yoshida Y., Sugiura T., Yamashita U., et al.
    • Journal Title

      Journal of Applied Toxicology

      Volume: 30 Pages: 656-660

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An indoor air pollutant 2-ethyl-hexanol activates CD4 cells.2009

    • Author(s)
      Yoshida Y., Liu J.Q., Sugiura T., Ishidao T., Ueno S., Yanagita H., Fueta Y., Kunugita N., Hori H., Yamashita U.
    • Journal Title

      Chemico-Bio Intera 177

      Pages: 137-141

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro.2009

    • Author(s)
      Takahashi N., Yoshida Y., Sugiura T., Matsuno K., Fujino A, Yamashita U.
    • Journal Title

      International Immunopharmacology 9

      Pages: 508-513

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formaldehyde inhalation activates signal transducer and activator of transcription 5 in immune cells.2009

    • Author(s)
      Yoshida Y., Ding N., Liu JQ., Noguchi J., Sugiura T., Kunugita N.
    • Journal Title

      European Immunology Conference 39

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The NF-kappaB RelA subunit confers resistance to Leishmania major by inducing nitric oxide synthase 2 and Fas expression but not Th1 differentiation.2009

    • Author(s)
      Omata S., Kuroda E., Sugiura T., Yamashita U.
    • Journal Title

      J Immunol 182

      Pages: 4910-4916

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of 1-bromopropane, a substitute for chlorofluorocarbons2009

    • Author(s)
      Yoshida Y., Nakano Y., Ueno S., Liu JQ., Fueta Y., Ishidao T., Kunugita N., Yanagihara N., Sugiura T., Yamashita U., Hori H.
    • Journal Title

      on brain-derived neurotrophic factor (BDNF) expression Int ImmPharma 9

      Pages: 433-438

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The indoor air pollutant 2-ethyl-hexanol activates CD4 cells.2009

    • Author(s)
      Yoshida, Y., 劉冀琴, 杉浦勉, 石田尾徹, 上野晋, 笛田由紀子, 欅田尚樹, 保利一, 山下優毅
    • Journal Title

      Chemico-Bio Intera 177

      Pages: 137-141

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro.2009

    • Author(s)
      Takahashi, N., Yoshida, Y., Sugiura, T., Masuno K., Fujino, A, Yamashita, U
    • Journal Title

      International Immunopharmacology 9

      Pages: 508-513

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formaldehyde inhalation activates signal transducer and activator of transcription 5 in immune cells.2009

    • Author(s)
      Yoshida, Y., Ding, N., Liu, JQ., Noguchi, J., Sugiura, T., Kunugita, N.
    • Journal Title

      European Immunology Conference 39

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The NF-kappaB RelA subunit confers resistance to Leishmania major by inducing nitric oxide synthase 2 and Fas expression but not Th1 differe ntiation.2009

    • Author(s)
      Omata, S., Kuroda, E., Sugiura, T., Yamashita, U.
    • Journal Title

      J Immunol 182

      Pages: 4910-4916

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of 1-bromopropane, a substitute for chlorofluorocarbons, on brain-derived neurotrophic factor (BDNF) expression2009

    • Author(s)
      Yoshida, Y., Nakano, Y., Ueno, S., Liu, JQ., Fueta, Y., Ishidao, T., Kunugita, N., Yanagihara, N., Sugiura, T., Yamashita, U., Hori, H.
    • Journal Title

      Int ImmPharma 9

      Pages: 433-438

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of prostaglandin E2 synthesis and microsomal prosta-glandin E synthase-1 expression in microglia by glioma-derived soluble factors.2008

    • Author(s)
      Nakano Y., Kuroda E., Kito T., Uematsu S., Akira S., Yokota A., Nishizawa S., Yamashita U.
    • Journal Title

      J. Neurosurgery 108

      Pages: 311-319

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous hypersensitivities to hapten are controlled by IFN-gamma-upregulated keratinocyte Th1 chemokines and IFN-gamma-downregulated langerhans cell Th2 chemokines.2008

    • Author(s)
      Mori T, Kabashima K., Yoshiki R., Sugita K., Shiraishi N., Onoue A., Kuroda E., Kobayashi M., Yamashita U.
    • Journal Title

      J.Invest.Dermatol. 128

      Pages: 1719-1727

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of prostaglandin E_2 synthesis and microsomal prosta-glandin E synthase-1 expression in microglia by glioma-derived soluble factors.2008

    • Author(s)
      Y. Nakano, et al.
    • Journal Title

      J. Neurosurgery 108

      Pages: 311-319

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous hypersensitivities to hapten are controlled by IFN-gamma-upregulated keratinocyte Th1 chemokines and IFN-gamma-downregulated langerhans cell Th2 chemokines.2008

    • Author(s)
      T. Mori, et al.
    • Journal Title

      J. Invest. Dermatol. 128

      Pages: 1719-1727

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA-binding activity of NF-kB and phosphorylation of p65 are induced by N-acetylcysteine through phosphatidylinositol (PI) 3-kinase2008

    • Author(s)
      J. Q. Liu, et al.
    • Journal Title

      Molecular Immunology 45

      Pages: 3984-3989

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] BDNF produced by leukemia cells attenuates cytokine production in macrophages and induced Foxp3 positive cells through NF-AT, Smad and RhoA.2010

    • Author(s)
      Yoshida Y., Tsukada J.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Anti-proliferative activity of cucurbitacin D on T-cell leukemia through proteasome inhibition.2010

    • Author(s)
      Ding N. Yamashita U., Yoshida Y.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Oxidative stress and apoptotic changes in mouse macrophages exposed to cadmium.2010

    • Author(s)
      Sugiura T., 山下優毅
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] BDNF produced by leukemia cells attenuates cytokine production in macrophages and induced Foxp3 positive cells through NF-AT, Smad and RhoA.2010

    • Author(s)
      Yoshida, Y., Tsukada, J.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸(神戸国際会議場)
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Anti-proliferative activity of cucurbitacin D on T-cell leukemia through proteasome inhibition.2010

    • Author(s)
      Ding, N.Yamashita, U., Yoshida, Y.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸(神戸国際会議場)
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Oxidative stress and apoptotic changes in mouse macrophages exposed to cadmium.2010

    • Author(s)
      Sugiura, T., Yamashita, U.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸(神戸国際会議場)
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of mechanism of the IL -1 induction by new proteasome inhibitor Cucurbitacin D.2010

    • Author(s)
      吉田安宏
    • Organizer
      Bit's 3rd. Annual World Cancer Congress 2010
    • Place of Presentation
      Singapore
    • Year and Date
      2010-06-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of mechanism of the IL-1 induction by new proteasome inhibitor Cucurbitacin D2010

    • Author(s)
      Yoshida, Y.
    • Organizer
      Bit's 3rd Annual World Cancer Congress 2010
    • Place of Presentation
      シンガポール(シンガポールEXPO)
    • Year and Date
      2010-06-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] BDNF produced by leukemia cells modulates immune responses.2010

    • Author(s)
      吉田安宏
    • Organizer
      1st. World Congress of Immunodiseases and Therapy.
    • Place of Presentation
      Beijing
    • Year and Date
      2010-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] BDNF produced by leukemia cells modulates immune responses2010

    • Author(s)
      Yoshida, Y.
    • Organizer
      1st.World Congress of Immunodiseases and Therapy
    • Place of Presentation
      中国・北京(北京国際コンベンションセンター)
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Induction of apoptosis in T-cell leukemia by new drug which targeted a proteasome inhibition.2009

    • Author(s)
      Yoshida Y., Ding N., Yamashita U.
    • Organizer
      World Congress of Gene-2009
    • Place of Presentation
      中国・佛山
    • Year and Date
      2009-12-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Induction of apoptosis in T-cell leukemia by new drug which targeted aproteasome inhibition.2009

    • Author(s)
      Yoshida, Y., Ding, N., Yamashita, U.
    • Organizer
      World Congress of Gene-2009
    • Place of Presentation
      中国・佛山
    • Year and Date
      2009-12-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cucurbitacin Dの転写および転写後修飾によるIL-1β産生の調節機構2009

    • Author(s)
      丁寧,吉田安宏,山下優毅
    • Organizer
      第39回日本免疫学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] BDNF produced by leukemia cells activates AP1 and NF-AT, which resulted in induction of Foxp3.2009

    • Author(s)
      吉田安宏,丁寧,山下優毅
    • Organizer
      第39回日本免疫学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] カドミウムにより誘導される酸化傷害に対するレドックス制御におけるメタロチオネインの役割2009

    • Author(s)
      杉浦勉,山下優毅
    • Organizer
      第39回日本免疫学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] BDNF produced by leukemia cells activates AP1 and NF-AT, which resulted in induction of Foxp3.2009

    • Author(s)
      吉田安宏, 丁〓, 山下優毅
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪(大阪国際会議場)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] カドミウムにより誘導される酸化傷害に対するレドックス制御におけるメタロチオネインの役割2009

    • Author(s)
      杉浦勉, 山下優毅
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪(大阪国際会議場)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cucurbitacin Dの転写および転写後修飾によるIL-1β産生の調節機構2009

    • Author(s)
      Ding, N., 吉田安宏, 山下優毅
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪(大阪国際会議場)
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Triterpenoids cucurbitacin D isolated from cucurbitaceae induces apoptosis in adult T-cell leukemia through proteasome inhibition. 20092009

    • Author(s)
      Yoshida Y., Ding N., Yamashita U.
    • Organizer
      4th Medical Biotech Forum, International experts symposium
    • Place of Presentation
      大連
    • Year and Date
      2009-08-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Triterpenoids cucurbitacin D isolated from cucurbitaceae induces apoptosis in adult T-cell leukemia through proteasome inhibition.2009

    • Author(s)
      Yoshida, Y., Ding, N., Yamashita, U.
    • Organizer
      2009 4th Medical Biotech Forum, International experts symposium
    • Place of Presentation
      中国・大連
    • Year and Date
      2009-08-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] プロスタグランジンD2による免疫反応の調節2008

    • Author(s)
      山下優毅,丁寧,吉田安宏
    • Organizer
      第26回産業医科大学学会総会
    • Place of Presentation
      北九州市
    • Year and Date
      2008-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] プロスタグランジンD_2による免疫反応の調節2008

    • Author(s)
      山下優毅, 丁宇, 吉田安宏
    • Organizer
      第26回産業医科大学学会総会
    • Place of Presentation
      北九州市
    • Year and Date
      2008-10-30
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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