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A role of MMP family molecules in remodeling ECM and advancing calcification

Research Project

Project/Area Number 20592134
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTohoku University

Principal Investigator

SASANO Yasuyuki  Tohoku University, 大学院・歯学研究科, 教授 (30196191)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Megumi  東北大学, 大学院・歯学研究科, 助教 (20431512)
AKITA Hirotoshi  東北大学, 大学院・歯学研究科, 助教 (10108540)
Co-Investigator(Renkei-kenkyūsha) SUZUKI Osamu  東北大学, 大学院・歯学研究科, 教授 (60374948)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords骨 / 石灰化 / 器官培養 / カルシウム / 原子吸光分光光度計 / エネルギー分散型X線分析装置 / MMP / ラット / 骨発生 / 骨修復 / 頭蓋骨 / 下顎骨 / 元素濃度 / GM6001 / DMSO / リン / 炭素
Research Abstract

An organ culture system to model the physiological calcification process was designed using rat embryonic calvaria as a device for analyzing its mechanism. Standardized calvarial explants were dissected from rat embryos aged day 18 and cultured for 1, 3, and 5 days. The Ca content was examined with atomic absorption spectrophotometry and distribution of Ca and the concentration of Ca, P and C were examined with von Kossa stain and/or SEM-EDX. The result indicates that the organ culture system using E18 calvaria is useful for modeling of the physiological calcification process in vitro.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2011 2010 2009 2008

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (19 results) Book (1 results)

  • [Journal Article] Root and periodontal tissue development after allogenic tooth transplantation between rat littermates.2011

    • Author(s)
      Andou K, Nakamura M, Ina Y, Sasaki K, Sasano Y
    • Journal Title

      Oral Diseases (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fibroblast growth factor 10 regulates Meckel's cartilage formation during early mandibular morphogenesis in rats.2011

    • Author(s)
      Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
    • Journal Title

      Dev Biol (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Root and periodontal tissue development after allogenic tooth transplantation between rat littermates.2011

    • Author(s)
      Andou K, 他
    • Journal Title

      Oral Diseases

      Volume: 未定(in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fibroblast growth factor 10 regulates Meckel's cartilage formation during early mandibular morphogenesis in rats.2011

    • Author(s)
      Terao F, 他
    • Journal Title

      Dev Biol

      Volume: 未定(in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of collagen types I and II on articular cartilage in a rat knee contracture model.2010

    • Author(s)
      Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, Takahashi I, Sasano Y, Onoda Y, Suda H, Itoi E
    • Journal Title

      Connective Tissue Research 51

      Pages: 22-30

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of collagen types I and II on articular cartilage in a rat knee contracture model.2010

    • Author(s)
      Hagiwara Y, 他
    • Journal Title

      Connective Tissue Research 51

      Pages: 22-30

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy.2009

    • Author(s)
      Kaneko R, Akita H, Shimauchi H, Sasano Y
    • Journal Title

      J Electron Microsc 58(6)

      Pages: 363-373

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects.2009

    • Author(s)
      Ebina H, Hatakeyama J, Onodera M, Honma T, Kamakura S, Shimauchi H, Sasano Y
    • Journal Title

      Oral Diseases 15

      Pages: 273-280

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts.2009

    • Author(s)
      Sato S, Tsuchiya M, Komaki K, Kusunoki S, Tsuchiya S, Haruyama N, Takahashi I, Sasano Y, Watanabe M
    • Journal Title

      Histochemistry Cell Biol 131

      Pages: 583-591

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of mechanical stress response during chondrogenesis.2009

    • Author(s)
      Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
    • Journal Title

      Journal of Biomechanical Science and Engineering 4

      Pages: 307-317

    • NAID

      130000129002

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy.2009

    • Author(s)
      Kaneko R, 他
    • Journal Title

      J Electron Microsc 58(6)

      Pages: 363-373

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of mechanical stress response during chondrogenesis.2009

    • Author(s)
      Takahashi I, 他
    • Journal Title

      Biomechanical Science and Engineering 4

      Pages: 307-317

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Micro-CT qnalysis of alveolar bone healing using a rat experimental model of critical-size defects2009

    • Author(s)
      Ebina H, 他
    • Journal Title

      Oral Diseases 15

      Pages: 273-280

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and intracellular transportation of type I procollagen during functional differentiation of odontoblasts2009

    • Author(s)
      Sato S, 他
    • Journal Title

      Histochemistry Cell Biol 131

      Pages: 583-591

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Root elongation and periodontal tissue formation in tooth germsallogenically transplanted between rat littermates.2011

    • Author(s)
      Andou K, 他
    • Organizer
      The 4th International Synposium for Interface Oral Health Science
    • Place of Presentation
      仙台市
    • Year and Date
      2011-03-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats.2011

    • Author(s)
      Kato A, 他
    • Organizer
      The 4th International 5ynposium for Interface Oral Health Science
    • Place of Presentation
      仙台市
    • Year and Date
      2011-03-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Root elongation and periodontal tissue formation in tooth germs allogenically transplanted between rat littermates.2011

    • Author(s)
      Andou K, Nakamura M, Ina Y, Sasaki K, Sasano Y
    • Organizer
      The 4th International Synposium for Interface Oral Health Science.
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats.2011

    • Author(s)
      Kato A, Nakamura M, Echigo S, Sasano Y
    • Organizer
      The 4th International Synposium for Interface Oral Health Science.
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] 硬組織の発生と修復における細胞外基質のリモデリング2010

    • Author(s)
      笹野泰之
    • Organizer
      歯科基礎医学会学術大会 サテライトシンポジウム
    • Place of Presentation
      東京都江戸川区船堀
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] カプサイシンを利用した骨修復における感覚神経の役割の検討2010

    • Author(s)
      加藤晶子、中村恵、越後成志、笹野泰之
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      盛岡市
    • Related Report
      2010 Annual Research Report 2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 硬組織の発生と修復における細胞外基質のリモデリング2010

    • Author(s)
      笹野泰之
    • Organizer
      第52回歯科基礎医学会学術大会 サテライトシンポジウム
    • Place of Presentation
      東京都江戸川区船堀
    • Related Report
      2010 Final Research Report
  • [Presentation] マイクロCTを利用した同腹他個体ラット間移植における歯根伸長の検討2009

    • Author(s)
      安藤浩二, 他
    • Organizer
      第55回東北・北海道連合支部学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bone formation and ECM remodeling cease within a limited period regardless of completion of bone healing in the rat calvarial defect.2009

    • Author(s)
      Sasano Y
    • Organizer
      Workshop, 8^<th> Pan-Pacific Connective Tissue Societies Symposium.
    • Place of Presentation
      神奈川県
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] The physiological calcification process is replicated in a rat embryonic calvarial culture2009

    • Author(s)
      Kimura Y, 他
    • Organizer
      The 3^<rd> International Synposium for Interface Oral Health Science
    • Place of Presentation
      仙台市
    • Year and Date
      2009-01-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] The physiological calcification process is replicated in a rat embryonic calvarial culture.2009

    • Author(s)
      Kimura Y, Kikunaga S, Takahashi I, Hatakeyama Y. Fukumoto S, Sasano Y
    • Organizer
      The 3rd International Synposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Immunohistological study on STRO-1 in developing rat dental tissues with light and electron microscopy.2009

    • Author(s)
      Kaneko R, Akita H, Shimauchi H, Sasano Y
    • Organizer
      The 3rd International Synposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Bone formation and ECM remodeling cease within a limited period regardless of completion of bone healing in the rat calvarial defect.2009

    • Author(s)
      笹野泰之
    • Organizer
      Workshop, 8th Pan-Pacific Connective Tissue Societies Symposium.
    • Place of Presentation
      Shonan Village Center, Kanagawa, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] カプサイシンの全身投与が知覚および三叉神経線維の形態に及ぼす影響2009

    • Author(s)
      加藤晶子、中村恵、越後成志、笹野泰之
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 同腹他個体ラット間の根未完成歯移植における歯根伸長と歯周組織形成に関する検討2009

    • Author(s)
      安藤浩二、中村恵、伊奈慶典、佐々木啓一、笹野泰之
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] マイクロCTを利用した同腹他個体ラット間移植における歯根伸長の検討2009

    • Author(s)
      安藤浩二、中村恵、笹野泰之
    • Organizer
      第55回東北・北海道連合支部学術集会
    • Place of Presentation
      仙台市
    • Related Report
      2010 Final Research Report
  • [Presentation] 胎生期ラット頭蓋骨器官培養系における骨基質石灰化進行過程の検討2008

    • Author(s)
      木村泰子、笹野泰之
    • Organizer
      第54回東北・北海道連合支部学術集会
    • Place of Presentation
      郡山市
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 骨基質の石灰化進行を再現する器官培養系の検討2008

    • Author(s)
      木村泰子、菊永茂司、高橋一郎、畠山雄次、福本敏、笹野泰之
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京都
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] I型コラーゲン発現を指標とした硬組織形成細胞のin vivoでの活性の検討2008

    • Author(s)
      笹野泰之
    • Organizer
      ワークショップ「組織細胞化学からみた硬組織研究の新展開」第49回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      長崎市
    • Related Report
      2010 Final Research Report
  • [Book] Interface Oral Health Science 2009(Sasano T, Suzuki O (eds))(The physiological calcification process is replicated in a rat embryonic calvarial culture.)2009

    • Author(s)
      Kimura Y, Kikunaga S, Takahashi I, Hatakeyama Y, Fukumoto S, Sasano Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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