A role of MMP family molecules in remodeling ECM and advancing calcification
| Project/Area Number |
20592134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SASANO Yasuyuki Tohoku University, 大学院・歯学研究科, 教授 (30196191)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Megumi 東北大学, 大学院・歯学研究科, 助教 (20431512)
AKITA Hirotoshi 東北大学, 大学院・歯学研究科, 助教 (10108540)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Osamu 東北大学, 大学院・歯学研究科, 教授 (60374948)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 骨 / 石灰化 / 器官培養 / カルシウム / 原子吸光分光光度計 / エネルギー分散型X線分析装置 / MMP / ラット / 骨発生 / 骨修復 / 頭蓋骨 / 下顎骨 / 元素濃度 / GM6001 / DMSO / リン / 炭素 |
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| Research Abstract |
An organ culture system to model the physiological calcification process was designed using rat embryonic calvaria as a device for analyzing its mechanism. Standardized calvarial explants were dissected from rat embryos aged day 18 and cultured for 1, 3, and 5 days. The Ca content was examined with atomic absorption spectrophotometry and distribution of Ca and the concentration of Ca, P and C were examined with von Kossa stain and/or SEM-EDX. The result indicates that the organ culture system using E18 calvaria is useful for modeling of the physiological calcification process in vitro.
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Report
(4 results)
Research Products
(34 results)
