Intranuclear action of Cell cycle-specific growth inhibitory factor, CDT

• Project/Area Number: 20592140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SUGAI Motoyuki Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (10201568)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔細菌 / CDT / 細胞死 / 細胞周期 / 歯周病 / Aggregatibactor actinomycetemcomitans / Aggregatibacter actinomycetemcomitans / periodontal diseases / oral / DNase / homologous recombination / apoptosis / Dnase / DNA / アポトーシス

Research Abstract

When CdtB was expressed in yeat, Cdt acted as cytolethal and inhibited cell cycle at S/G2. Effect of mutation of DNA repair system on CDT-induced cell death was studied. Mutants of ead51, mre11, rad50 and wrs2 were found to be highly sensitive to CdtB. Further effect of mutation of apoptotic pathway on CDT-induced cell death was studied. Mutants of yca1 and aif1 did not change their susceptibility to CdtB. These resuts suggest that Cdt-induced yeast cell death is not dependent on apoptosis.

Research Products

• [Journal Article] Topical application of Aggregatibacter actinomycetemcomitans CDT induces cell cycle arrest in the rat gingival epithelium in vivo. (2011)
  • Author(s): Masaru Ohara, Mutsumi Miyauchi, Keiko Tsuruda, Takashi Takata, Motoyuki Sugai.
  • Journal Title: Journal of Periodontal Research 46 Pages: 389-395
  • Peer Reviewed
• [Journal Article] Topical application of Aggregatibacter actinomycetemcomitans CDT induces cell cycle arrest in the rat gingival epithelium in vivo (2011)
  • Author(s): Ohara, M., et al.
  • Journal Title: Journal of Periodontal Research Volume: 46 Pages: 389-395
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin from Aggregatibacter actinomycetemcomitans induces DNA damage, S/G2 cell cycle arrest and caspase-independent death in budding yeast model. (2010)
  • Author(s): Oranart Matangkasombut, Roongtiwa Wattanawaraporn, Keiko Tsuruda, Masaru Ohara, Motoyuki Sugai, Skorn Mongkolsuk.
  • Journal Title: Infection and Immunity 78 Pages: 783-792
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin from Aggregatibacter actinomycetemcomitans induces DNA dmage, S/G2 cell cycle arrest, and caspase-independent death in a Saccharomyces cerevisiae model (2010)
  • Author(s): Oranart Matangkasombut, et al.
  • Journal Title: Infection and Immunity 78 Pages: 783-792
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin type I and type IV genes are framed with lambdoid prophage genes in extraintestinal pathogenic Escherichia coli. (2009)
  • Author(s): Toth I, Nougayrede JP, Dobrindt U, Ledger TN, Boury M, Morabito S, Fujiwara T, Sugai M, Hacker J, Oswald E.
  • Journal Title: Infection and Immunity 77(1) Pages: 492-500
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin type I and type IV genes are framed with lamboid prophage genes in extraintestinal pathogenic Escherichia coli (2009)
  • Author(s): Istvan Toth, et al.
  • Journal Title: Infection and Immunity 77 Pages: 492-500
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 and Jurkat cells. (2008)
  • Author(s): Ohara, M., Hayashi, T., Kusunoki, Y., Nakachi, K., Fujiwara, T., Komatsuzawa, H., Sugai, M.
  • Journal Title: Infection and Immunity 76(10) Pages: 4783-4791
  • Peer Reviewed
• [Journal Article] Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells (2008)
  • Author(s): Ohara M., et al.
  • Journal Title: Infection and Immunity 76 Pages: 4783-4791
  • Peer Reviewed
• [Presentation] Using yeast as a model to study the mechanisms of bacterial Cytolethal Distendina Toxin (2010)
  • Author(s): Oranart Matangkasombut
  • Organizer: 2^<nd> International Workshop on Biodental Education & Research
  • Place of Presentation: Hiroshima City
  • Year and Date: 2010-02-12
• [Presentation] Cytolethal distending toxin from Aggregatibacter actinomycetemcomitans induces DNA damage, S/G2 cell cycle arrest and caspase-independent death in budding yeast model. (2009)
  • Author(s): Matangkasombut, O., Wattanawaraporn, R., Tsuruda, K., Ohara, M., Sugai, M., Mongkolsuk, S.
  • Organizer: 3^<rd> Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima