| Project/Area Number |
20592145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
KUSAMA Kaoru Meikai University, 歯学部, 教授 (20130479)
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| Co-Investigator(Kenkyū-buntansha) |
SAKASHITA Hideaki 明海大学, 歯学部, 教授 (10178551)
田中 章夫 明海大学, 歯学部, 助教 (30406392)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKASHITA Hideaki 明海大学, 歯学部, 教授 (10178551)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 口腔病理学 / 口腔癌 / 扁平上皮癌 / 上皮間葉移行 / E-cadherin / vimentin / TGF-β / Snail / podoplanin / podolanin / E-カドヘリン / ビメンチン / mRNA |
|---|
| Research Abstract |
To clarify the association of epithelial-mesenchymal transition (EMT) with progression of oral squamous cell carcinoma (OSCC), the expression of transforming growth factor β (TGF-β), Snail, E-cadherin and vimentin was examined in 57 cases of oral SCC by an immunohistochemical method. TGF-β expression was demonstrated in 77.2% of cases, Snail in 63.2% and vimentin in 15.8%, respectively. Reduction or loss of E-cadherin expression was found in all cases. Localization of vimentin expression was overlapped with the reduction or loss of E-cadherin expression, but not with that of Snail expression. The expression of TGF-β, E-cadherin and vimentin was not correlated with any clinicopathological findings or invasive front grading (IFG). Analyses by real time RT-PCR using cell lines derived from head and neck cancers revealed the increased vimentin mRNA expression in Ca9-22 cells and significantly decreased E-cadherin mRNA expression in KB cells were demonstrated. HSC-3 cells showed slight increased Snail mRNA expression, but there was no change of vimentin and E-cadherin mRNA expressions. These findings suggest that TGF-β induced-EMT is involved in the progression of oral SCC, and reduction of E-cadherin expression may be associated with transcriptional repression factors other than Snail. EMT in OSCC occurred differently in each case and there were some OSCC cases which podoplanin expression is involved in the progression.
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