Regulation mechanisms of development, growth and degeneration of craniofacial embryonic tissues.

• Project/Area Number: 20592146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Meikai University
• Principal Investigator: AMANO Osamu Meikai University, 歯学部, 教授 (60193025)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 発生 / 熱ショックタンパク / アポトーシス / メッケル軟骨 / 甲状舌管 / 歯肉 / 甲状腺 / 脂肪酸結合タンパク / 細胞増殖 / 軟骨吸収 / 骨端板軟骨 / 軟骨内骨化 / 熱ショック蛋白質 / Hsp27 / Hsp25 / 歯胚

Research Abstract

The following findings were obtained in the development and degenerative processes of mouse Meckel's cartilage : apoptosis was not found in Meckel's chondrocytes ; many apoptotic cells were found in the perichondrium of lateral growing aspect of the cartilage during the developing period ; and organ-cultured mouse embryonic mandibles supplemented with an apoptotic depressant showed partial deficiency of Meckel's cartilage. These results strongly suggested that the apoptosis is involved in the differentiation of chondroblasts in the perichondrim and is essential for the morphogenesis of Meckel's cartilage.

Research Products

• [Journal Article] Localization of heat shock protein 27 (hsp27) in the rat gingiva and its changes with tooth eruption. (2011)
  • Author(s): Sasaki A, Yamada T, Inoue K, Momoi T, Tokunaga H, Sakiyama K, Kanegae H, Suda N, Amano O
  • Journal Title: Acta Histochem Cytochem 44 Pages: 17-24
  • NAID: 130000395312
  • Peer Reviewed
• [Journal Article] Chemical constituents of Lycoris albiflora and their cytotoxic activities. (2011)
  • Author(s): Jitsuno M, Yokosuka A, Hashimoto K, Amano O, Sakagami H, Mimaki Y
  • Journal Title: Nat Prod Commun 6 Pages: 187-192
  • Peer Reviewed
• [Journal Article] Localization of heat shock protein 27 (hsp27) in the rat gingiva and its changes with tooth eruption. (2011)
  • Author(s): Sasaki A, Yamada T, Inoue K, Momoi T, Tokunaga H, Sakiyama K, Kanegae H, Suda N, Amano O.
  • Journal Title: Acta Histochem Cytochem. Volume: 44 Pages: 17-24
  • NAID: 130000395312
  • Peer Reviewed
• [Journal Article] Chemical constituents of Lycoris albiflora and their cytotoxic activities. (2011)
  • Author(s): Jitsuno M, Yokosuka A, Hashimoto K, Amano O, Sakagami H, Mimaki Y.
  • Journal Title: Nat Prod Commun. Volume: 6 Pages: 187-92
  • Peer Reviewed
• [Journal Article] Meckel's cartilage : Discovery, embryology and evolution. (2010)
  • Author(s): Amano O, Doi T, Yamada T, Sasaki A, Sakiyama K, Kanegae H, Kindaichi K
  • Journal Title: J Oral Biosci 52 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by benzaldehyde. (2010)
  • Author(s): Ariyoshi-Kishino K, Hashimoto K, Amano O, Saitoh J, Kochi M, Sakagami H
  • Journal Title: Anticancer Res 30 Pages: 5069-5076
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by benzaldehyde. (2010)
  • Author(s): Ariyoshi-Kishino K, Hashimoto K, Amano O, Saitoh J, Kochi M, Sakagami H.
  • Journal Title: Anticancer Res. Volume: 30 Pages: 5069-5076
  • Peer Reviewed
• [Journal Article] Meckel's cartilage : Discovery, embryology and evolution (2010)
  • Author(s): Amano, O., et al.
  • Journal Title: J.Oral Biosci. 52(印刷中)
  • Peer Reviewed
• [Journal Article] Cell death induced by nutritional starvation in mouse macrophage-like RAW264.7 cells. (2009)
  • Author(s): Sakagami H, Kishino K, Amano O, Kanda Y, Kunii S, Yokote Y, Oizumi H, Oizumi T
  • Journal Title: Anticancer Res 29(1) Pages: 343-347
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by gefitinib in oral squamous cell carcinoma cell lines. (2009)
  • Author(s): Chu Q, Amano O, Kanda Y, Kunii S, Wang Q, Sakagami H
  • Journal Title: Anticancer Res 29(12) Pages: 5023-5031
  • Peer Reviewed
• [Journal Article] Non-apoptotic cell death induced by nutritional starvation in J774.1 mouse macrophage-like cell line. (2009)
  • Author(s): Thet MM, Yokote Y, Chu Q, Wang QT, Kishino K, Tamura N, Shimada J, Amano O, Kanda Y, Kunii S, Takekawa F, Hatano H, Shimada C, Hasegawa H, Sakagami H
  • Journal Title: Anticancer Res 29(12) Pages: 5083-5088
  • Peer Reviewed
• [Journal Article] マウス胎仔の臼歯歯胚における熱ショックタンパク質Hsp27の局在 (2009)
  • Author(s): 佐々木会, 山田亨, 土居孝資, 岡安麻里, 徳永寛司, 鐘ヶ江晴秀, 天野修
  • Journal Title: 明海歯科医学 38 Pages: 58-66
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by gefitinib in oral squamous cell carcinoma cell lines (2009)
  • Author(s): Chu, Q., Amano, O., et al.
  • Journal Title: Anticancer Res. 29 Pages: 5023-2031
  • Peer Reviewed
• [Journal Article] Cell death induced by nutritional starvation in mouse macrophage-like RAW264.7 cells (2009)
  • Author(s): Sakagami, H., Kishino, K., Amano, O., et al.
  • Journal Title: Anticancer Res. 29 Pages: 343-347
  • Peer Reviewed
• [Journal Article] マウス胎仔の臼歯歯胚における熱ショックタンパク質Hsp27の局在 (2009)
  • Author(s): 佐々木 会, 他
  • Journal Title: 明海歯科医学 38 Pages: 58-66
  • Peer Reviewed
• [Journal Article] Cell death induced by nitritional starvation in mouse macrophage-like RAW264.7 cells (2009)
  • Author(s): Sakagami, H., et al.
  • Journal Title: Anticancer Research 29 Pages: 343-348
  • Peer Reviewed
• [Journal Article] メッケル軟骨の発生,消失とその役割 (2008)
  • Author(s): 天野修
  • Journal Title: 小児口腔外科 18 Pages: 1-8
  • NAID: 130004356847
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by beta-cyclodextrin benzaldehyde inclusion compound. (2008)
  • Author(s): Liu Y, Sakagami H, Hashimoto K, Kikuchi H, Amano O, Ishihara M, Kanda Y, Kunii S, Kochi M, Zhang W, Yu G
  • Journal Title: Anticancer Res 28(1A) Pages: 229-236
  • Peer Reviewed
• [Journal Article] Glutathione can efficiently prevent direct current-induced cytotoxicity (2008)
  • Author(s): Nakamura Y, Shimetani A, Fujii H, Amano O, Sakagami H, Takahashi K
  • Journal Title: J Endod 34(6) Pages: 693-697
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by benzocycloheptoxazines in human tumor cell lines. (2008)
  • Author(s): Murayama H, Miyahara K, Wakabayashi H, Kurihara T, Hashimoto K, Amano O, Kikuchi H, Nakamura Y, Kanda Y, Kunii S, Motohashi N, Sakagami H
  • Journal Title: Anticancer Res 28(2A) Pages: 1069-1078
  • NAID: 120005520596
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by peplomycin in oral squamous cell carcinoma cell lines. (2008)
  • Author(s): Liu Y, Sakagami H, Amano O, Kikuchi H, Nakamura Y, Ishihara M, Kanda Y, Kunii S, Zhang W, Yu G
  • Journal Title: Anticancer Res 28(4B) Pages: 2197-2204
  • Peer Reviewed
• [Journal Article] Tumor-specific cytotoxicity and type of cell death induced by sodium 5,6-benzylidene-L-ascorbate. (2008)
  • Author(s): Kishino K, Hashimoto K, Amano O, Kochi M, Liu WK, Sakagami H
  • Journal Title: Anticancer Res 28(5A) Pages: 2577-2584
  • Peer Reviewed
• [Journal Article] メッケル軟骨の発生, 消失とその役割 (2008)
  • Author(s): 天野 修
  • Journal Title: 小児口腔外科 18 Pages: 1-8
  • NAID: 130004356847
  • Peer Reviewed
• [Presentation] Development and degeneration of mouse thyroglossal duct. (2011)
  • Author(s): Inoue K, Bando Y, Takizawa S, Momoi T, Sakiyama K, Sakashita H, Amano O.
  • Organizer: 第116回日本解剖学会総会・第88回日本生理学会総会合同学会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-03-28
• [Presentation] Localization of epidermal-type fatty acid binding protein (E-FABP) in the tibial epiphyseal plates of mice. (2010)
  • Author(s): Bando Y, Sakiyama K, Inoue K, Adachi Y, Owada Y, Amano O
  • Organizer: The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama
• [Presentation] Development and degeneration of mouse thyroglossal duct. (2010)
  • Author(s): Inoue K, Bando Y, Takizawa S, Momoi T, Sakiyama K, Sakashita H, Amano O
  • Organizer: The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama
• [Presentation] Localization of heart-type fatty acid-binding protein in oral muscles of mice. (2010)
  • Author(s): Momoi T, Bando Y, Inoue K, Sakiyama K, Owada Y, Suda N, Amano O
  • Organizer: The 116th Annual Meeting of the Japanese Association of Anatomists
  • Place of Presentation: Yokohama
• [Presentation] IGF遺伝子導入による培養筋芽細胞の特性変化 (2010)
  • Author(s): 崎山浩司, 瀧澤将太, 坂東康彦, 天野修
  • Organizer: 第47回日本口腔組織培養学会学術大会
  • Place of Presentation: 高知
• [Presentation] 唾液腺の解剖・組織からHSPまで (2010)
  • Author(s): 天野修
  • Organizer: 第34回日本口腔外科学会教育研修会
  • Place of Presentation: 大阪府
• [Presentation] マウスメッケル軟骨の軟骨膜におけるアポトーシス (2010)
  • Author(s): 土居孝資, 桃井知子, 井上勝元, 佐々木会, 崎山浩司, 鐘ヶ江晴秀, 天野修
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] マウスメッケル軟骨におけるpodoplaninの局在 (2010)
  • Author(s): 桃井知子, 崎山浩司, 井上勝元, 土居孝資, 佐々木会, 鐘ヶ江晴秀, 天野修
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] メカニカルストレスを付与したラット歯根膜でのHsp27・Hsp 47の局在と変動 (2010)
  • Author(s): 佐々木会, 土居孝資, 井上勝元, 桃井知子, 崎山浩司, 鐘ヶ江晴秀, 天野修
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] マウス甲状舌管の発生と消失におけるHsp27の局在 (2010)
  • Author(s): 井上勝元, 佐々木会, 土居孝資, 桃井知子, 崎山浩司, 坂下英明, 天野修
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] BadとCAはNaF誘導性アポトーシスに関与する (2010)
  • Author(s): 杉山完司, 中島敏明, 天野修
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡市
• [Presentation] 唾液腺の解剖・組織からHSPまで (2010)
  • Author(s): 天野修
  • Organizer: 第33回日本口腔外科学会教育研修会
  • Place of Presentation: 東京
• [Presentation] 口腔顎顔面の組織発生・再生における熱ショック蛋白の役割. (2009)
  • Author(s): 天野 修
  • Organizer: 第21回日本歯科医学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-11-04
• [Presentation] α,β-不飽和ケトン類は,ヒト口腔扁平上皮癌細胞にミトコンドリアの障害を伴う細胞死を誘導する (2009)
  • Author(s): 坂上宏, 天野修
  • Organizer: 第46回日本口腔組織培養学会学術大会
  • Place of Presentation: 東京
• [Presentation] NaF誘導性アポトーシスにおけるBadタンパク質の関与 (2009)
  • Author(s): 大槻純子, 杉山完司, 天野修, 安井利一, 坂上宏
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] 熱ショックタンパク質27のマウス甲状舌管における局在 (2009)
  • Author(s): 井上勝元, 土居孝資, 佐々木会, 崎山浩司, 坂下英明, 天野修
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] マウスメッケル軟骨の軟骨膜におけるアポトーシス (2009)
  • Author(s): 土居孝資, 佐々木会, 井上勝元, 崎山浩司, 鐘ヶ江晴秀, 天野修
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] 舌癌が周囲筋線維に与える影響 (2009)
  • Author(s): 崎山浩司, 井上勝元, 土居孝資, 佐々木会, 岩沼治, 天野修
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] ベンズアルデヒドによりヒト口腔扁平上皮癌細胞に誘導される細胞死の解析 (2009)
  • Author(s): 岸野香織, 橋本研, 天野修, 坂上宏
  • Organizer: 第51回歯科基礎医学会学術大会・総会
  • Place of Presentation: 新潟
• [Presentation] 矯正力を付与したラット歯根膜でのHsp27・Hsp47の局在と変動 (2009)
  • Author(s): 佐々木会, 土居孝資, 井上勝元, 桃井知子, 崎山浩司, 鐘ヶ江晴秀, 天野修
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
• [Presentation] マウスMeckel軟骨におけるpodoplaninの局在 (2009)
  • Author(s): 桃井知子, 崎山浩司, 井上勝元, 鐘ヶ江晴秀, 天野修
  • Organizer: 第51回日本組織細胞化学会学術集会
  • Place of Presentation: 大津
• [Presentation] 矯正力を付与したラット歯根膜でのHsp27の局在・変動 (2009)
  • Author(s): 佐々木会, 真野樹子, 成田亜希子, 松井成幸, 天野修, 鐘ヶ江晴秀
  • Organizer: 第68回矯正歯科学会大会
  • Place of Presentation: 東京
• [Presentation] 解剖学者メッケルの一族 (2009)
  • Author(s): 天野修
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
• [Presentation] マウス舌癌周囲筋組織の筋線維特性 (2009)
  • Author(s): 崎山浩司, 井上勝元, 岩沼治, 阿部伸一, 井出吉信, 天野修
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
• [Presentation] マウス胎仔の甲状舌管の発生と消失における熱ショックタンパク質25の局在 (2009)
  • Author(s): 井上勝元, 佐々木会, 土居孝資, 桃井知子, 崎山浩司, 坂下英明, 天野修
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
• [Presentation] マウスメッケル軟骨の軟骨膜におけるアポトーシスの役割 (2009)
  • Author(s): 土居孝資, 山田亨, 佐々木会, 井上勝元, 鐘ヶ江晴秀, 天野修
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
• [Presentation] 歯の萌出に伴う口腔粘膜上皮におけるHsp27の局在の変化と細胞増殖との関連 (2009)
  • Author(s): 佐々木会, 山田亨, 土居孝資, 井上勝元, 桃井知子, 鐘ヶ江晴秀, 天野修
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
• [Presentation] 口腔顎顔面の組織発生・再生における熱ショック蛋白の役割 (2008)
  • Author(s): 天野修
  • Organizer: 第21回日本歯科医学会総会
  • Place of Presentation: 横浜
• [Book] 歯・口腔の構造と機能 (2011)
  • Author(s): 天野修, 他
  • Publisher: 医歯薬出版
• [Book] 歯・口腔の構造と機能 口腔解剖学・口腔組織発生学・口腔生理学 (2011)
  • Author(s): 天野修, 他
  • Total Pages: 276
  • Publisher: 医歯薬出版
• [Book] 人体の構造と機能 (2010)
  • Author(s): 天野修, 他
  • Publisher: 医歯薬出版
• [Book] 口腔解剖学(井出吉信,前田健康,天野修編) (2009)
  • Author(s): 天野修, 他
  • Publisher: 医歯薬出版
• [Book] 口腔解剖学 (2009)
  • Author(s): 天野修, 他
  • Total Pages: 212
  • Publisher: 医歯薬出版
• [Book] Current Experimental Study for Treatment of Cleft Lip and Palate (2008)
  • Author(s): Shida, Y., et al. (分担, 共同執筆)
  • Publisher: Quintessence Publishing