Molecular biological analysis of periodontopathic biofilm formation and its restraints by functional proteins

• Project/Area Number: 20592150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: KATO Tetsuo Tokyo Dental College, 歯学部, 教授 (00159253)
• Co-Investigator(Kenkyū-buntansha): KIMIZUKA Ryuta 東京歯科大学, 歯学部, 講師 (90287178) ; 岡田 あゆみ 東京歯科大学, 歯学部, 助教 (40231667)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細菌 / 歯学 / 歯周病 / バイオフィルム / 機能性タンパク質

Research Abstract

Dental plaque, a microbial biofilm formed by multiple organisms tightly adhered to the tooth surface, plays a key role in the development of periodontal disease. The effect of functional proteins on the periodontopathic biofilm formation was determined using the flat bottom of 96-well cell culture plates. Cystatin significantly inhibited biofilm formation by some periodontopathic bacterial strains. Galectins markedly suppressed IL-6 induction in human umbilical vascular endothelial cells by periodontopathic bacterial endotoxin.

Research Products

• [Journal Article] Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides (2010)
  • Author(s): Yoshinari M., Kato T., Matsuzaka K., Hayakawa T., Shiba K.
  • Journal Title: Biofouling 26 Pages: 103-110
  • Peer Reviewed
• [Journal Article] Antimicrobial activity of super-oxidised water against oral microorganisms (2010)
  • Author(s): Yamada K., Yama M., Takaku Y., Kakizawa T., Kimizuka R., Okuda K., Kato T.
  • Journal Title: Arch.Oral Biol. 55 Pages: 397-400
  • Peer Reviewed
• [Journal Article] Investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction (2010)
  • Author(s): Komiya-Ito A., Ishihara K., Tomita S., Kato T., Yamada S.
  • Journal Title: Bull.Tokyo Dent.Coll. 51 Pages: 139-144
  • NAID: 130004471694
  • Peer Reviewed
• [Journal Article] Bactericidal effects of 2.94 μm and 1.67 μm laser (2010)
  • Author(s): Kukidome N., Amagai T., Osuka K., Kato J., Hirai Y., Kato T., Aida S.
  • Journal Title: Bull.Tokyo Dent.Coll. 51 Pages: 185-192
  • Peer Reviewed
• [Journal Article] Basic peptide protamine exerts antimicrobial activity against periodontopathic bacteria (2010)
  • Author(s): Miura T., Iohara K., Kato T., Ishihara K., Yoshinari M.
  • Journal Title: J.Biomed.Sci.Engi. 3 Pages: 1069-1072
  • Peer Reviewed
• [Journal Article] Antimicrobial activity of super-oxidised water against oral microorganisms (2010)
  • Author(s): Yamada, K.
  • Journal Title: Arch.Oral Biol. Volume: 55 Pages: 397-400
  • Peer Reviewed
• [Journal Article] Investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction (2010)
  • Author(s): Komiya-Ito, A.
  • Journal Title: Bull.Tokyo Dent.Coll. Volume: 51 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Basic peptide protamine exerts antimicrobial activity against periodontopathic bacteria (2010)
  • Author(s): Miura, T.
  • Journal Title: J.Biomed.Sci.Engi. Volume: 3 Pages: 1069-1072
  • Peer Reviewed
• [Journal Article] Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides (2010)
  • Author(s): Yoshinari, M.
  • Journal Title: Biofouling 26 Pages: 103-110
  • Peer Reviewed
• [Journal Article] Congo red-binding protein in rough-phenotype Aggregatibacter actinomycetemcomitans is amyloid-like fiber (2009)
  • Author(s): Kimizuka R., Kato T. Hashimoto S., Yamanaka-Okada A., Okuda K., Ishihara K.
  • Journal Title: Bull.Tokyo Dent.Coll. 50 Pages: 23-29
  • NAID: 130004471668
  • Peer Reviewed
• [Journal Article] Effect of eel galectin AJL-1 on periodontopathic bacterial biofilm formation and their lipopolysaccharide-mediated inflammatory cytokine induction (2009)
  • Author(s): Takayama S., Saitoh E., Kimizuka R., Yamada S., Kato T.
  • Journal Title: Int.J.Antimicrob. Agents 34 Pages: 355-359
  • Peer Reviewed
• [Journal Article] Inhibitory effect of galectin-3 on cytokine-inducing activity of periodontopathic Aggregatibacter actinomycetemcomitans endotoxin in splenocytes derived from mice (2009)
  • Author(s): Kato T., Uzawa A., Ishihara K.
  • Journal Title: FEMS Immunol. Med.Microbiol. 57 Pages: 40-45
  • Peer Reviewed
• [Journal Article] Congo red-binding protein in rough-phenotype Aggregatibacter actinomycetemcomitans is amyloid-like fiber (2009)
  • Author(s): Kimizuka, R.
  • Journal Title: Bull. Tokyo dent. Coll. 50 Pages: 23-29
  • NAID: 130004471668
  • Peer Reviewed
• [Journal Article] Effect of eel galectin AJL-1 on periodontopathic bacterial biofilm formation and their lipopolysaccharide-mediated inflammatory cytokine induction (2009)
  • Author(s): Takayama, S.
  • Journal Title: Int.J.Antimicrob. Agents 34 Pages: 355-359
  • Peer Reviewed
• [Journal Article] Inhibitory effect of galectin-3 on cytokine-inducing activity of periodontopathic Aggregatibacter actinomycetemcomitans endotoxin in splenocytes derived from mice (2009)
  • Author(s): Kato, T.
  • Journal Title: FEMS Immunol. Med.Microbiol. 57 Pages: 40-45
  • Peer Reviewed
• [Journal Article] Relationship between antimicrobial protein levels in whole saliva and periodontitis (2008)
  • Author(s): Ito T., Komiya-Ito A, Arataki T., Furuya Y., Yajima Y., Yamada S., Okuda K., Kato T.
  • Journal Title: J.Periodontol. 79 Pages: 316-322
  • Peer Reviewed
• [Journal Article] Nicotine involved in periodontal disease through influence on cytokine levels (2008)
  • Author(s): Makino A., Yamada S., Okuda K., Kato T.
  • Journal Title: FEMS Immunol.Med.Microbiol. 52 Pages: 282-286
  • Peer Reviewed
• [Journal Article] Inhibitory effect of cranberry polyphenol on cariogenic bacteria (2008)
  • Author(s): Yamanaka-Okada A., Sato E., Kouchi T., Kimizuka R., Kato T., Okuda K.
  • Journal Title: Bull.Tokyo Dent.Coll. 49 Pages: 107-112
  • NAID: 130004868634
  • Peer Reviewed
• [Journal Article] Relationship between antimicrobial protein levels in whole saliva and periodontitis (2008)
  • Author(s): Ito, T.
  • Journal Title: J. Periodontol. 79 Pages: 316-322
  • Peer Reviewed
• [Journal Article] Nicotine involved in periodontal disease through influence on cytokine levels (2008)
  • Author(s): Makino, A.
  • Journal Title: FEMS Immunol. Med. Microbiol. 52 Pages: 282-286
  • Peer Reviewed
• [Journal Article] Inhibitory effect of cranberry polyphenol on cariogenic bacteria (2008)
  • Author(s): Yamanaka-Okada, A.
  • Journal Title: Bull. Tokyo dent. Coll. 49 Pages: 107-112
  • Peer Reviewed
• [Presentation] チタンインプラント埋入時の免疫応答-サイトカインプロフィール-、歯科学報 (2010)
  • Author(s): 小田貴士、西村孝太、伊藤太一、加藤哲男、吉成正雄、矢島安朝
  • Organizer: 第290回東京歯科大学学会総会(110 493)
  • Place of Presentation: 千葉
  • Year and Date: 2010-10-16
• [Presentation] チタンインプラント埋入時の免疫応答-サイトカインプロフィール- (2010)
  • Author(s): 小田貴士
  • Organizer: 第290回東京歯科大学学会総会
  • Place of Presentation: 東京歯科大学千葉校舎(千葉県)
  • Year and Date: 2010-10-16
• [Presentation] 黄色ブドウ球菌V8プロテアーゼ刺激によるA549細胞のIL-8産生 (2010)
  • Author(s): 平井要
  • Organizer: 第52回歯科基礎医学会総会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2010-09-22
• [Presentation] Effect of ethanol on galectin-3 production by human endothelial cells, J.Dent.Res.89 (Special Issue B) (2010)
  • Author(s): Kato T., Kimizuka R., Ishihara, K.
  • Organizer: 88th general session of the International Association for Dental Research
  • Place of Presentation: Barcelona(abstract4832)
  • Year and Date: 2010-07-17
• [Presentation] Effect of ethanol on galectin-3 production by human endothelial cells (2010)
  • Author(s): Kato T.
  • Organizer: 88th general session of the International Association for Dental Research
  • Place of Presentation: Centre Conventions International Barcelona (Spain)
  • Year and Date: 2010-07-17
• [Presentation] Identification of amyloid-like fiber in rough-phenotype Aggregatibacter actinomycetemcomitans, J.Dent.Res.89 (Special Issue B) (2010)
  • Author(s): Kimizuka R., Kato T., Ishihara K.
  • Organizer: 88th general session of the International Association for Dental Research
  • Place of Presentation: Barcelona(abstract2626)
  • Year and Date: 2010-07-16
• [Presentation] Identification of amyloid-like fiber in rough-phenotype Aggregatibacter a ctinomycetemcomitans (2010)
  • Author(s): Kimizuka, R.
  • Organizer: 88th general session of the International Association for Dental Research
  • Place of Presentation: Centre Conventions International Barcelona(Spain)
  • Year and Date: 2010-07-16
• [Presentation] シスタチンSAのPorphyromonas gingivalisおよびAggregatibacter actinomycetemcomitansにおける増殖抑制、歯科学報 (2010)
  • Author(s): 加藤哲男、小澤誠、石原和幸、斎藤英一
  • Organizer: 東京歯科大学創立120周年記念学術講演会第289回東京歯科大学学会(110 385)
  • Place of Presentation: 東京
  • Year and Date: 2010-05-08
• [Presentation] Aggregatibacter actinomycetemcomitans rough型集落形成株のアミロイド様物質産生、歯科学報 (2010)
  • Author(s): 君塚隆太、加藤哲男、石原和幸
  • Organizer: 東京歯科大学創立120周年記念学術講演会第289回東京歯科大学学会(110 429)
  • Place of Presentation: 東京
  • Year and Date: 2010-05-08
• [Presentation] 黄色ブドウ球菌V8プロテアーゼによるA549細胞からのIL-8の誘導、日細菌誌 (2010)
  • Author(s): 平井要、菊池有一郎、上田青海、柴田幸永、大久保裕一郎、平岡行博、加藤哲男、石原和幸、藤村節夫
  • Organizer: 第83回日本細菌学会総会(65 194)
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-27
• [Presentation] 黄色ブドウ球菌V8プロテアーゼ刺激によるA549細胞のIL-8産生、J.Oral Biosci. (2010)
  • Author(s): 平井要、菊池有一郎、上田青海、柴田幸永、平岡行博、加藤哲男、石原和幸、藤村節夫
  • Organizer: 第52回歯科基礎医学会総会(52(Supple.) 183)
  • Place of Presentation: 東京
• [Presentation] シスタチンSAのPorphyromonas gingivalisおよびAggregatibacter actinomycetemcomitansにおける増殖抑制 (2010)
  • Author(s): 加藤哲男
  • Organizer: 第289回東京歯科大学学会例会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] Aggregatibacter actinomycetemcomitans rough型集落形成株のアミロイド様物質産生 (2010)
  • Author(s): 君塚隆太
  • Organizer: 第289回東京歯科大学学会例会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] ヒト内皮細胞のガレクチンー3産生におよぼすエタノールの影響、J.Oral Biosci. (2009)
  • Author(s): 加藤哲男、君塚隆太、石原和幸
  • Organizer: 第51回歯科基礎医学会総会(51(Supple.) 134)
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] ヒト内皮細胞のガレクチンー3産生におよぼすエタノールの影響 (2009)
  • Author(s): 加藤哲男
  • Organizer: 第51回歯科基礎医学会総会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-09-11
• [Presentation] 歯周病原細菌LPSの炎症性サイトカイン誘導能に対するガレクチン-3の抑制効果、歯科学報 (2009)
  • Author(s): 加藤哲男、高山沙織、君塚隆太、石原和幸
  • Organizer: 第287回東京歯科大学学会例会(109 243)
  • Place of Presentation: 千葉
  • Year and Date: 2009-06-06
• [Presentation] 歯周病原細菌LPSの炎症性サイトカイン誘導能に対するガレクチン-3の抑制効果 (2009)
  • Author(s): 加藤哲男
  • Organizer: 第287回東京歯科大学学会例会
  • Place of Presentation: 東京歯科大学千葉校舎
  • Year and Date: 2009-06-06
• [Presentation] Aggregatibacter actinomycetemcomitans rough型集落形成株のアミロイド形成、日細菌誌 (2009)
  • Author(s): 君塚隆太、加藤哲男、石原和幸
  • Organizer: 第82回日本細菌学会総会(64 185)
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 歯周病原細菌LPSのサイトカイン誘導に対するガレクチンの影響、J.Oral Biosci. (2008)
  • Author(s): 加藤哲男、高山沙織、君塚隆太、石原和幸
  • Organizer: 第50回歯科基礎医学会総会(50(Supple.) 205)
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Rough型Aggregatibacter actinomycetemcomitansはアミロイド様物質を形成する、J.Oral Biosci. (2008)
  • Author(s): 君塚隆太、加藤哲男、岡田あゆみ、橋本貞充、石原和幸
  • Organizer: 第50回歯科基礎医学会総会(50(Supple.) 213)
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] 歯周病原細菌LPSのサイトカイン誘導に対するガレクチンの影響 (2008)
  • Author(s): 加藤哲男
  • Organizer: 第50回歯科基礎医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Rough型Aggregatibacter actinomycetemcomitansはアミロイド様物質を形成する (2008)
  • Author(s): 君塚隆太
  • Organizer: 第50回歯科基礎医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Antibacterial effects of eel galectin on periodontopathic bacteria, J.Dent.Res.87 (Special Issue A) (2008)
  • Author(s): Takayama S., Kimizuka R., Saitoh E., Yamada S., Okuda K., Kato T.
  • Organizer: 86th general session of the International Association for Dental Research
  • Place of Presentation: Toronto(CD-ROM1396)
  • Year and Date: 2008-07-04
• [Presentation] Antibacterial effects of eel galectin on periodontopathic bacteria (2008)
  • Author(s): Takayama, S.
  • Organizer: 86th general session of the International Association for Dental Research
  • Place of Presentation: Toronto
  • Year and Date: 2008-07-04
• [Presentation] ウナギガレクチンの歯周病予防における有効性、歯科学報 (2008)
  • Author(s): 高山沙織、加藤哲男、君塚隆太、小川貴也、奥田克爾、山田了
  • Organizer: 第285回東京歯科大学学会例会(108 182)
  • Place of Presentation: 千葉
  • Year and Date: 2008-06-07
• [Presentation] ウナギガレクチンの歯周病予防における有効性 (2008)
  • Author(s): 高山沙織
  • Organizer: 第285回東京歯科大学学会例会
  • Place of Presentation: 千葉
  • Year and Date: 2008-06-07
• [Book] 最新口腔微生物学 (2009)
  • Author(s): 奥田克爾、石原和幸、加藤哲男(共著)
  • Total Pages: 477
  • Publisher: 一世出版
• [Book] 最新口腔微生物学 (2009)
  • Author(s): 奥田克爾
  • Total Pages: 477
  • Publisher: 一世出版