Mechanism of salivary secretion and activation of paracellular pathway.
Project/Area Number |
20592152
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Tokyo Dental College |
Principal Investigator |
HASHIMOTO Sadamitsu Tokyo Dental College, 歯学部, 准教授 (10201708)
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Co-Investigator(Renkei-kenkyūsha) |
MURAKAMI Masataka 生理学研究所, 細胞器官研究系, 准教授 (10104275)
SHIMONO Masaki 東京歯科大学, 歯学部, 教授 (00085771)
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Research Collaborator |
SUGIYA Hiroshi 日本大学, 生物資源学部, 教授 (20050114)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 唾液腺 / 唾液分泌 / タイト結合 / 細胞骨格 / 傍細胞経路 / isoproterenol / carbachol / 凍結割断法 |
Research Abstract |
To elucidate the morphological change of the paracellular route for fluid secretion, the cytoskeleton of TJs were examined by TEM, using deep etching freeze-fracture (FF) replicas. Isolated submandibular glands (SMGs) were stimulated with 1 μM of carbachol (CCh) and isoproterenol (IPR). After CCh/IPR stimulation, cytoskeletal filaments beneath the plasma membrane were arranged in a thicker layer. Contraction of the submembranous actin cytoskeleton during exocytosis elicited by CCh/IPR may cause rearrangement of TJ strands, and modulate increased paracellular permeability.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium.2010
Author(s)
Sugisawa, M., Masaoka, T., Enokiya, Y., Muramatsu, T., Hashimoto, S., Yamada, S., Shimono, M.
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Journal Title
J Periodont Res
Volume: 45(2)
Pages: 284-291
Related Report
Peer Reviewed
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[Journal Article] Down-regulated genes in mouse dental papillae and pulp.2010
Author(s)
Sasaki, H., Muramatsu, T., Kwon, H.J., Yamamoto, S., Hashimoto, S., Jung, H.S., Shimono, M.
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Journal Title
J Dent Res.
Volume: 89(7)
Pages: 679-683
Related Report
Peer Reviewed
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[Journal Article] Sodium-calcium exchangers in rat ameloblasts.2010
Author(s)
Okumura, R., Shibukawa, Y., Muramatsu, T., Hashimoto, S., Nakagawa, K., Tazaki, M., Shimono, M.
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Journal Title
J Pharmacol Sci.
Volume: 112(2)
Pages: 223-230
NAID
Related Report
Peer Reviewed
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[Journal Article] Proliferation, migration and apoptosis of periodontal ligament cells after tooth replantation.2010
Author(s)
Sato, K., Muramatsu, T., Tsuchiya, Y., Masaoka, T., Enokiya, Y., Hashimoto, S., Shimono, M.
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Journal Title
Oral Dis.
Volume: 16(3)
Pages: 263-268
Related Report
Peer Reviewed
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[Journal Article] Electrophysiological properties of AQP6 in mouse parotid acinar cells.2009
Author(s)
Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S. Momose Y., Tazaki M., Shimono M., Sugiya H.
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Journal Title
J.Medical Investigation Proceeding 56
Pages: 347-349
NAID
Related Report
Peer Reviewed
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[Journal Article] Electrophysiological properties of AQP6 in mouse parotid acinar cells.2009
Author(s)
Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S., Momose Y., Tazaki M., Shimono M., Sugiya H.
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Journal Title
J. Medical Investigation 56(Sup)
Pages: 347-349
NAID
Related Report
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[Presentation] Electrophysiological properties of AQP6 in mouse parotid acinar cells.2009
Author(s)
Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S., Momose Y., Tazaki M., Shimono M., Sugiya H.
Organizer
ISES, The 11th International Symposium on Exocrine Secretion
Place of Presentation
Tokushima, Japan
Related Report
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[Presentation] Electrophysiological properties of AQP6 in mouse parotid acinar cells.2009
Author(s)
Ichikawa H., Shibukawa Y., Sahara Y., Tsumura M., Qi B., Satoh K., Narita T., Hashimoto S., Momose Y., Tazaki M., Shimono M., Sugiya H.
Organizer
ISES, The 11th International Symposium on Exocrine Secretion, Tokushima 09,(外分泌腺機能国際シンポジウム,徳島09 '外分泌-メカニズムと病気')
Place of Presentation
徳島
Related Report
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