| Project/Area Number |
20592152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
HASHIMOTO Sadamitsu Tokyo Dental College, 歯学部, 准教授 (10201708)
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| Co-Investigator(Renkei-kenkyūsha) |
MURAKAMI Masataka 生理学研究所, 細胞器官研究系, 准教授 (10104275)
SHIMONO Masaki 東京歯科大学, 歯学部, 教授 (00085771)
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| Research Collaborator |
SUGIYA Hiroshi 日本大学, 生物資源学部, 教授 (20050114)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 唾液腺 / 唾液分泌 / タイト結合 / 細胞骨格 / 傍細胞経路 / isoproterenol / carbachol / 凍結割断法 |
|---|
| Research Abstract |
To elucidate the morphological change of the paracellular route for fluid secretion, the cytoskeleton of TJs were examined by TEM, using deep etching freeze-fracture (FF) replicas. Isolated submandibular glands (SMGs) were stimulated with 1 μM of carbachol (CCh) and isoproterenol (IPR). After CCh/IPR stimulation, cytoskeletal filaments beneath the plasma membrane were arranged in a thicker layer. Contraction of the submembranous actin cytoskeleton during exocytosis elicited by CCh/IPR may cause rearrangement of TJ strands, and modulate increased paracellular permeability.
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