Basic research of the antigen delivery system that it aimed at the optimization of the DNA vaccine for cancer prevention

• Project/Area Number: 20592166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: MAEDA Hatsuhiko Aichi Gakuin University, 歯学部, 教授 (30175591)
• Co-Investigator(Kenkyū-buntansha): KUBO Katsutoshi 愛知学院大学, 歯学部, 准教授 (60329604)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: DNAワクチン / パピローマウイルス / ナノスフェアー / ドラッグデリバリー / 癌抑制効果 / 口腔 / 扁平上皮癌 / naked pDNA / ハムスター / DNA ワクチン

Research Abstract

Many oral mucous diseases are related to human papillomavirus (HPV). Recently it has become clear that more potent methods for DNA vaccine delivery need to be developed to enhance the efficacy of DNA vaccines. In this study, the best antigen delivery system was examined for the purpose of preventing HPV infection by using the DNA vaccine. The results showed that the immunization with PLGA nanosphere DNA vaccines followed by EP in vivo delayed carcinoma development of papillomavirus-associated oral cancer. Both of in vivo electroporation and PLGA nanosphere are potent methods for DNA vaccine delivery, therefore, it contributes to the papillomavirus-induced oral cancer prevention.

Research Products

• [Journal Article] Human papillomaviruses in oral carcinoma and oral potentially malignant disorders : a systematic review. (2011)
  • Author(s): S Syrjanen, G Lodi, I von Bultzingslowen, A Aliko, P Arduino, G Campisi, S Challacombe, G Ficarra, C Flaitz, HM Zhou, H Maeda, C Miller, M Jontell
  • Journal Title: Oral Dieseases 17(Suppl.1) Pages: 58-72
  • Peer Reviewed
• [Journal Article] ヒトパピローマウイルス(HPV)と口腔粘膜疾患 (2010)
  • Author(s): 前田初彦
  • Journal Title: 日本口腔外科学会雑誌 56(8) Pages: 464-470
  • NAID: 10027843392
  • Peer Reviewed
• [Journal Article] ヒトパピローマウイルス(HPV)と口腔粘膜疾患 (2010)
  • Author(s): 前田初彦
  • Journal Title: 日本口腔外科学会雑誌 Volume: 56 Pages: 464-470
  • NAID: 10027843392
  • Peer Reviewed
• [Presentation] Enhancement of DNA vaccine potency against hamster oral papillomavirus-associated oral cancer by in vivo electroporation. (2010)
  • Author(s): Maeda H, Kubo K, Yoshida W, Torii R, Sato E, Jinno M, Takayama M, Wada A
  • Organizer: The 6th Asian Science Seminar in TAIWAN
  • Place of Presentation: 台湾
  • Year and Date: 2010-11-20
• [Presentation] nhancement of DNA vaccine potency against hamster oral papillomavirus-associated oral cancer by In Vivo electroporation (2010)
  • Author(s): H Maeda, K Kubo, W Yoshida, R Torii, E Sato, M Jinno, M Takayama, A Wada
  • Organizer: The 6^<th> Asian Science Seminar in TAIWAN
  • Place of Presentation: 中山、台湾
  • Year and Date: 2010-11-20
• [Presentation] Enhancement of DNA Vaccine Potency by In Vivo Electroporation (2010)
  • Author(s): Maeda H, Yoshida W, Kubo K, Sugita Y, Sato E, Suzumura Y, Torii R, Wada A, Komatsu S, Ito K, Saito T, Umebayashi T, Jinno M, Takayama M, Honda Y
  • Organizer: 10^<th> Biannial Conference, European Association of Oral Medicine
  • Place of Presentation: London, UK
  • Year and Date: 2010-09-24
• [Presentation] 癌化抑制DNAワクチンの最適化を目指した抗原デリバリーシステム (2010)
  • Author(s): 前田初彦
  • Organizer: 第9回バイオテクノロジー国際会議
  • Place of Presentation: 東京
  • Year and Date: 2010-07-01
• [Presentation] 口腔領域のHPV感染 (2010)
  • Author(s): 前田初彦
  • Organizer: 第32回大阪STI研究会・第1回日本性感染症学会関西支部学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-06-26
• [Presentation] 癌化抑制DNAワクチンのDDSとしての低電圧電気穿孔法の最適化を目指した基礎的研究 (2010)
  • Author(s): 前田初彦,久保勝俊,杉田好彦,佐藤恵美子,吉田和加,和田昭,高山光平,神野正人
  • Organizer: 第28回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-29
• [Presentation] 癌化抑制DNAワクチンのDDSとしての低電圧電気穿孔法の最適化を目指した基礎的研究 (2010)
  • Author(s): 前田初彦
  • Organizer: 第28回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 学術総合センター(東京都)
  • Year and Date: 2010-01-29
• [Presentation] Enhancement of DNA Vaccine Potency by In Vivo Electroporation. (2010)
  • Author(s): Maeda H, Yoshida W, Kubo K, Sugita Y, Sato E, Suzumura Y, Torii R, Wada A, Komatsu S, Ito K, Saito T, Umebayashi T, Jinno M, Takayama M, Honda Y
  • Organizer: The 10th EAOM (Biennial Conference of the European Association of Oral Medicine)
  • Place of Presentation: London
• [Presentation] In vivo electroporation enhnaces the DNA vaccine potency against hamster oral papillomavirus-associated oral cancer. (2008)
  • Author(s): Maeda H, Sugita Y, Kubo K, Sato E, Suzumura Y, Jinno M, Takayama K, Honda Y, Komori A
  • Organizer: 14^<th> International Congress, International Association of Oral Pathologists
  • Place of Presentation: San Francisco, California, USA
  • Year and Date: 2008-06-23
• [Presentation] In vivo electroporation enhances the DNA vaccine potency agaist hamster oral papillomavirus-associated oral cancer (2008)
  • Author(s): Hatsuhiko Maeda
  • Organizer: The 14th International Congress on Oral Pathology and Medicine
  • Place of Presentation: アメリカ合衆国, サンフランシスコ
  • Year and Date: 2008-06-23
• [Presentation] DNAワクチンの癌化抑制効果における電気穿孔法のDDSとしての有用性について (2008)
  • Author(s): 前田初彦,神野正人,高山光平,小森敦夫,本田由馬,芳山昌典,和田昭,吉田和加,杉田好彦,四ツ柳智久
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京