| Project/Area Number |
20592169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ISHISAKI Akira Iwate Medical University, 歯学部, 教授 (20356439)
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| Co-Investigator(Kenkyū-buntansha) |
飯塚 正 北海道大学, 大学院・歯学研究科, 准教授 (80168062)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 幹細胞 / 歯根膜 / 再生医療 / 再生医学 / 間葉系幹細胞 / 転写因子 / 歯学 / 細胞・組織 / 血管内皮細胞 / 骨芽細胞 / 細胞分化 |
|---|
| Research Abstract |
To elucidate molecular mechanisms underlying the regulation of proliferation and differentiation of mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL), we established two types of single cell-derived cultures (SCDCs) of rat ligament cells, one of which named as SCDC2 retained the ability of self-renewal and multipotency and another of which named as SCDC1 did not. Then, we comprehensively compared the genes expression status and phosphorylation status of intracellular molecules between SCDC1 and SCDC2. We found that among 54,000 target genes, around 600 genes were identified as differentially expressed genes between the two types of cells. In particular, we found that some transcription factors, which were previously reported as proliferation and differentiation regulators of several kinds of stem cells, were highly expressed in the SCDC2 cells. Moreover, we found that the FGF-induced- and TGF--induced-intracellular signaling played important roles on the regulation of proliferation and differentiation of the MSCs. Thus, we successfully elucidated the molecular mechanisms underlying the regulation of proliferation and differentiation of MSCs derived from PDL.
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