| Project/Area Number |
20592178
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
INENAGA Kiyotoshi Kyushu Dental College, 歯学部, 教授 (90131903)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Kentro 九州歯科大学, 歯学部, 助教 (40316154)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ピロカルピン / セビメリン / 唾液分泌 / 口渇 / 昇圧 / 昇圧反応 / 喉の渇き / 口腔乾燥 / ムスカリン受容体 / 口渇中枢 / 催唾剤 |
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| Research Abstract |
Pilocarpine and cevimeline induce salivary secretion and get wet in the oral cavity. We showed that intraperitoneally and intracerebroventricularly injected pilocarpine elicited water intake in rats and the induced behavior was suppressed by intracerebroventricularly preadministered atropine. The same applications of pilocarpine increased the number of c-Fos positive neurons in the subfornical organ, median nucleus of preoptic area, and organum vasculosum of lamina terminalis, which may constitute the thirst center in the brain. Pilocarpine directly affected and depolarized neurons in the subfornical organ of slice preparations. On the other hand, intraperitoneally injected cevimeline did not increase water intake but rather suppressed angiotensin II-induced water intake. Pilocarpine and cevimeline increased intracellular calcium concentration in acinar cells of the salivary gland in vitro preparations. The increment of intracellular calcium concentration with in vitro application was rather short-lasting than with in vivo application. Thus, pilocarpine and cevimeline show actions on acinar cells of the salivary gland through the same intracellular second messenger system. From these results, it is suggested that pilocarpine and cevimeline have different actions between the central nervous system and the peripheral organs.
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