| Project/Area Number |
20592185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
SUZUKI Keiko Showa University, 歯学部, 講師 (50119187)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shoji 昭和大学, 歯学部, 教授 (00111617)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 炎症性骨破壊 / 破骨細胞 / イメージング / 骨吸収治療薬 / インビボイメージング / Toll-like receptor / ケモカイン / 骨粗鬆症治療薬 / in vivo imaging / 共焦点顕微鏡 |
|---|
| Research Abstract |
In this study, we investigated the mobilization of osteoclast precursor cells (OCPs) in living mice, in which the interrelationship among different cell types are maintained, by using in vivo imaging techniques. Within 3 days of luciferase (Luc)-Tg OCPs injection, Luc-positive cells were detected in the inflammation site, secondary lymph nodes and spleen; and furthermore, expression of TNFalpha, IL-1beta, IL-6, MCP-1 and MIP-1alpha mRNAs were significantly increased in LPS- or Pam3-injected mice. These findings indicate that OCPs circulating in the bloodstream migrate into the local inflammation site through MCP-1/MIP-1alpha activation and can take part in the rapid and extensive bone destruction.
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