| Project/Area Number |
20592186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
INOUE Tomio Showa University, 歯学部, 教授 (70184760)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Shiro 昭和大学, 歯学部・口腔生理学教室, 助教 (60384187)
NAKAYAMA Kiyomi 昭和大学, 歯学部・口腔生理学教室, 助教 (00433798)
MOCHIZUKI Ayako 昭和大学, 歯学部・口腔生理学教室, 助教 (10453648)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 口腔生理学 / 咀嚼 / 協調運動 / 三叉神経運動ニューロン / 表情筋運動ニューロン / ケージドグルタミン酸 / 膜電位感受性色素 / パッチクランプ / 三叉神経運動核 / 顔面神経核 / プレモーターニューロン / 吸啜 / 三叉神経 / 協調機構 / 舌運動 / 顔面運動 |
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| Research Abstract |
Mastication is a highly coordinated movement of the jaw, tongue, lip and the cheek, in which food is hold by the tongue and cheek, and then food is crushed by the upper and lower jaws with closure of the lips. Motor commands for the coordinated movement patterns of the jaw, tongue, lip and the cheek are thought to be produced by the pattern generator for mastication ; however, the detail is not still clear. In the present study, we investigated regions in frontal brainstem slice preparation from neonatal rats where premotor neurons targeting for jaw-opening, jaw-closing and facial motoneurons were located, using voltage-sensitive dye, whole-cell patch-clamp recordings and laser photostimulation in order to examine the neural mechanisms underlying coordination of the movements of jaw and facial muscles. Electrical stimulation of the reticular formation located between the trigeminal motor nucleus and the facial nucleus elicited optical responses both in the trigeminal motor nucleus and the facial nucleus. Photostimulation of the reticular formation also evoked post synaptic currents (PSCs) in jaw-opening, jaw-closing and facial motoneurons; however, the region of which photostimulation evoked PSCs in these three kinds of motoneurons were different among them. These results suggest that premotor neurons targeting for jaw-opening, jaw-closing and facial motoneurons might be differentially located in the reticular formation between the trigeminal motor nucleus and the facial nucleus.
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