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Study of mechanisms for secretory granule exocytosis from salivary gland

Research Project

Project/Area Number 20592189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

IMAI Akane  The Nippon Dental University, 新潟生命歯学部, 准教授 (60180080)

Co-Investigator(Kenkyū-buntansha) NASHIDA Tomoko  日本歯科大学, 新潟生命歯学部, 准教授 (10133464)
SHIMOMURA Hiromi  日本歯科大学, 新潟生命歯学部, 教授 (40139259)
YOSHIE Sumio  日本歯科大学, 新潟生命歯学部, 教授 (30095278)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords唾液腺 / アミラーゼ分泌 / 開口分泌 / exocyst / Rab27 / Rabエフェクター / β刺激 / Rab-GAP / 局在 / Exocyst / Sec6 / Sec8 / 唾液分泌機構 / SNARE複合体 / 発現 / Rabタンパク質
Research Abstract

Small GTP-binding protein, Rab27, has been implicated in the regulation of different types of membrane trafficking, including melanosome transport in melanocytes and regulated secretion events in a wide variety of secretory cells. We have previously shown that Rab27 is involved in the control of isoproterenol (IPR)-induced amylase release from rat parotid acinar cells. Although Rab27 is predominantly localized on secretory granules under resting conditions, changes to its intracellular localization after β-stimulation have never been elucidated. The present study investigated IPR-induced redistribution of Rab27B in the parotid acinar cells, revealing translocation from secretory granules to the subapical region after 5 min of IPR treatment and then diffusion into the cytosol after 30 min of IPR treatment. Dissociation of Rab27B from the apical plasma membrane is probably mediated through the Rab GDP dissociation inhibitor (GDI) in the cytosol extracting GDP-bound Rab protein from membranes, as a dramatic increase in the amount of the Rab27B-GDI complex in the cytosol was observed 30 min after stimulation with IPR. These results indicate that, in parotid acinar cells, Rab27B is translocated, in a time-dependent manner, from secretory granules into the apical plasma membrane as a result of exposure to IPR, and then into the cytosol through binding with the GDI.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (57 results)

All 2011 2010 2009 2008 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (34 results) Book (1 results) Remarks (5 results)

  • [Journal Article] Evidence for amylase release by cyclin-dependent kinase 5 in the rat parotid.2011

    • Author(s)
      Shimomura H, Imai A, Nashida T
    • Journal Title

      Arch Biochem Biophys Vol.507

      Pages: 310-314

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for amylase release by cyclin-dependent kinase 5 in the rat parotid.2011

    • Author(s)
      Shimomura H., et al.
    • Journal Title

      Arch Biochem Biophys.

      Volume: 507 Pages: 310-314

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミニレビュー耳下腺腺房細胞における唾液分泌メカニズム2010

    • Author(s)
      今井あかね
    • Journal Title

      生化学 82巻

      Pages: 130-135

    • Related Report
      2010 Final Research Report
  • [Journal Article] Gene expression profiles of the three major salivary glands in rats.2010

    • Author(s)
      Nashida T, Sato R, Imai A, Shimomura H
    • Journal Title

      Biomedical Research Vol.31

      Pages: 387-399

    • NAID

      130004470766

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiles of the three major salivary glands in rats2010

    • Author(s)
      Nashida T., et al.
    • Journal Title

      Biomedical Research

      Volume: 31 Pages: 387-399

    • NAID

      130004470766

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 耳下腺腺房細胞における唾液分泌メカニズム2010

    • Author(s)
      今井あかね
    • Journal Title

      生化学 82

      Pages: 130-135

    • NAID

      10026300516

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Redistribution of small GTP-binding protein, Rab27B, in rat pard acin cellsafter stimulation with isoproterenol.2009

    • Author(s)
      Imai A, Yoshie S, Nashida T, Fukuda M, Shimomura H
    • Journal Title

      Eur J Oral Sci Vol.117

      Pages: 224-230

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Redistribution of Rab27-specific effector Slac2-c, but not Slp4-a, after isoproterenol-stimulation in rat parotid acinar cells.2009

    • Author(s)
      Imai A, Fukuda M, Yoshie S, Nashida T, Shimomua H
    • Journal Title

      Arch Oral Bol Vol.54

      Pages: 361-368

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of exocytotic protein expression and Ca2+-dependent peptide secretion in astrocytes.2009

    • Author(s)
      Paco S, Margel MA, Olkkonen VM, Imai A, Blasi J, Fischer-Colbrie R, Aguado F
    • Journal Title

      J Neurochem Vol.110

      Pages: 143-156

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transferrin secretory pathways in rat parotid acinar cells.2009

    • Author(s)
      Nashida T, Yoshie S, Imai A, Shimomura H
    • Journal Title

      Arch Biochem Biophys Vol.487

      Pages: 131-138

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical and immunological characterization of the two α-N-acetylgalactosaminidases from squid liver.2009

    • Author(s)
      Sadik G, Rashid Md H, Nashida T, Ishii K, Sato Y, Shiraishi T, Uda Y
    • Journal Title

      Biol Pharm Bull Vol.32

      Pages: 1469-1473

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Redistribution of small GTP-binding protein, Rab27B, in ratparotid acinar cells after stimulation with isoproterenol2009

    • Author(s)
      Imai A., et al.
    • Journal Title

      Eur J Oral Sci 117

      Pages: 224-230

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transferrin secretory pathways in rat parotid acinar cells2009

    • Author(s)
      Nashida T., et al.
    • Journal Title

      Arch Biochem Biophys 487

      Pages: 131-138

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of exocytotic protein expression and Ca2+-dependent peptide secretion in astrocytes2009

    • Author(s)
      Paco S., Imai A., et al.
    • Journal Title

      J Neurochem 110

      Pages: 143-156

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redistribution of Rab27-specific effector Slac2-c, but not Slp4-a, after isoproterenol-stimulation in rat parotid acinar cells2009

    • Author(s)
      Imai A., et al.
    • Journal Title

      Arch Oral Biol 54

      Pages: 361-368

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redistribution of small GTP-binding protein, Rab27B, in rat parotid acinar cells after stimulation with isoproterenol2009

    • Author(s)
      Imai A., et al.
    • Journal Title

      Eur J Oral Sci 117(in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unstimulated amylase secretion is proteoglycan-dependent in rat parotid acinar cells.2008

    • Author(s)
      Nashida T, Imai A, Yoshie S, Shimomura H, Yokosuka H, Kumakura M
    • Journal Title

      Arch Biochem Biophys Vol.469

      Pages: 165-173

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] NODマウス耳下腺におけるアクアポリン8の発現減少2010

    • Author(s)
      梨田智子,羽下麻衣子,吉江紀夫,今井あかね,下村浩巳
    • Organizer
      第83回日本生化学会合同大会(BMB2010)
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ラット耳下腺腺房細胞のExocystメンバーの検索と開口分泌に対する関与2010

    • Author(s)
      今井あかね,梨田智子,下村浩巳
    • Organizer
      第83回日本生化学会合同大会(BMB2010)
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] イソプロテレノール刺激時の耳下腺腺房細胞におけるEPI64のRab27-GTPase-activating protein(Rab27-GAP)としての働きと発現について2010

    • Author(s)
      今井あかね,吉江紀夫,石橋弘太郎,福田光則,羽下麻衣子,梨田智子,下村浩巳
    • Organizer
      第55回日本唾液腺学会
    • Place of Presentation
      文京学院大学(東京都)
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 耳下腺腺房細胞におけるRab-GTPase活性化タンパク質(EPI64)の働き2010

    • Author(s)
      今井あかね,吉江紀夫,羽下麻衣子,梨田智子,下村浩巳
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] NODマウス耳下腺におけるアクアポリンファミリーの発現2010

    • Author(s)
      梨田智子,吉江紀夫,今井あかね,羽下麻衣子,下村浩巳
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Gene expression profiles of the three major salivary glands in rats.2010

    • Author(s)
      佐藤律子,梨田智子,今井あかね,下村浩巳
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Gene expression of parotid glands in non-obese diabetic mouse.2010

    • Author(s)
      福島琢士,梨田智子,又賀泉
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] エナメル小柱をもつ唯一の非哺乳類Uromastyxの歯胚構造とアメロジェニン遺伝子2010

    • Author(s)
      石山巳喜夫,三上正人,岡俊哉,今井あかね,下村浩巳,大井田新一郎
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Gene expression of the three major salivary glands.2010

    • Author(s)
      Sato R, Nashida T, Imai A, Shimomura H
    • Organizer
      88th General Session and Exhibition of the IADR
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] EPI64 functions as a Rab27-GTPase-activating protein in parotid acinar cells.2010

    • Author(s)
      Imai A, Fukuda M, Yoshie S, Haga M, Ishibashi K, Nashida T, Shimomura H
    • Organizer
      88th General Session and Exhibition of the IADR
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Evidence of amylase release by CDK5 in rat parotid.2010

    • Author(s)
      下村浩巳, 今井あかね, 梨田智子
    • Organizer
      88th General Session and Exhibition of the IADR
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2010-07-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Evidence of amylase release by CDK5 in rat parotid2010

    • Author(s)
      Shimomura H., et al.
    • Organizer
      88th General Session and Exhibition of the IADR
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Gene expression of parotid glands in non-obese diabetic mouse.2010

    • Author(s)
      Fukushima T, Nashida T, Mataga I
    • Organizer
      88th General Session and Exhibition of the IADR
    • Place of Presentation
      バルセロナ(スペイン)
    • Year and Date
      2010-07-14
    • Related Report
      2010 Final Research Report
  • [Presentation] イソプロテレノール刺激による耳下腺腺房細胞のSec6とSec8の相互作用について2009

    • Author(s)
      今井あかね,梨田智子,下村浩巳
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県)
    • Year and Date
      2009-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット耳下腺トランスフェリンのトランスサイトーシスにおける細胞骨格の役割2009

    • Author(s)
      梨田智子,吉江紀夫,今井あかね,下村浩巳
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県)
    • Year and Date
      2009-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 鯨類アメロジェニン遺伝子の分子生物学的解析2009

    • Author(s)
      石山巳喜夫、三上正人,岡俊哉,今井あかね,下村浩巳,大井田新一郎
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県)
    • Year and Date
      2009-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] イソプロテレノール刺激による耳下腺腺房細胞のSec6とSec8の相互作用について2009

    • Author(s)
      今井あかね, 他
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市 朱鷺メッセ
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラット耳下腺トランスフェリンのトランスサイトーシスにおける細胞骨格の役割2009

    • Author(s)
      梨田智子, 他
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟市 朱鷺メッセ
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Secretory pathway of transferrin in rat parotid acinar cells.2008

    • Author(s)
      梨田智子., 今井あかね., 下村浩巳., 吉江紀夫.
    • Organizer
      第81回生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2008-12-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Secretory pathway of transferrin in rat parotid acinar cells2008

    • Author(s)
      梨田 智子, 他
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] β刺激後の耳下腺腺房におけるRab27B、Slac2-cおよび Slp4-aの細胞内局在について2008

    • Author(s)
      今井あかね,吉江紀夫,福田光則,梨田智子,下村浩巳
    • Organizer
      第81回生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2008-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] β刺激後の耳下腺腺房におけるRab27B、Slac2-cおよびSlp4-aの細胞内局在について2008

    • Author(s)
      今井あかね, 他
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット耳下腺腺房細胞におけるExocystメンバーの検索と相互作用について2008

    • Author(s)
      今井あかね,梨田智子,下村浩巳
    • Organizer
      第50回歯科基礎医学会年会
    • Place of Presentation
      TOC有明コンベンションホール(東京都)
    • Year and Date
      2008-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット耳下腺からのトランスフェリン非刺激分泌経路の解明2008

    • Author(s)
      梨田智子,今井あかね,下村浩巳,吉江紀夫
    • Organizer
      第50回歯科基礎医学会年会
    • Place of Presentation
      TOC有明ベンションホール(東京都)
    • Year and Date
      2008-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 唾液腺における転写因子Mist1 mRNAの発現2008

    • Author(s)
      佐藤律子,梨田智子,今井あかね
    • Organizer
      第50回歯科基礎医学会年会
    • Place of Presentation
      TOC有明ベンションホール(東京)
    • Year and Date
      2008-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Uromastyxにおけるエナメル小柱の存在とアメロジェニン遺伝子発現の選択的splicingとの関連2008

    • Author(s)
      石山巳喜夫、三上正人,岡俊哉,今井あかね,下村浩巳,大井田新一郎
    • Organizer
      第50回歯科基礎医学会年会
    • Place of Presentation
      TOC有明ベンションホール(東京)
    • Year and Date
      2008-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット耳下腺腺房細胞におけるExocystメンバーの検索と相互作用について2008

    • Author(s)
      今井あかね, 他
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      TOC有明コンベンションホール
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット耳下腺からのトランスフェリン非刺激分泌経路の解明2008

    • Author(s)
      梨田 智子, 他
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      TOC有明コンベンションホール
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] 唾液腺における転写因子Mistl mRNAの発現2008

    • Author(s)
      佐藤 律子, 他
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      TOC有明コンベンションホール
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] Uromastyxにおけるエナメル小柱の存在とアメロジェニン遺伝子発現の選択的splicingとの関連2008

    • Author(s)
      石山 巳喜夫, 他
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      TOC有明コンベンションホール
    • Year and Date
      2008-09-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] Isoproterenol-dependent redistribution of Rab27, Slac2-c and Slp4-a in parotid glands.2008

    • Author(s)
      IMAI A., YOSHIE S., FUKUDA M., YOKOSUKA H., NASHIDA T., SHIMOMURA H.
    • Organizer
      86'th General Session of IADR
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Secretion of transferrin from rat parotid acinar cells.2008

    • Author(s)
      NASHIDA T., YOSHIE S., IMAI A., SHIMOMURA H., YOKOSUKA H.
    • Organizer
      86'th General Session of IADR
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2008-07-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Isoproterenol-dependent redistribution of Rab27, Slac2-c and Slp4-a in parotid glands2008

    • Author(s)
      Imai A., et al.
    • Organizer
      86^<th> General Session & Exhibition of the IADR
    • Place of Presentation
      カナダ・トロント
    • Year and Date
      2008-07-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Secretion of transferrin from rat parotid acinar cells2008

    • Author(s)
      Nashida T., et al.
    • Organizer
      86^<th> General Session & Exhibition of the IADR
    • Place of Presentation
      カナダ・トロント
    • Year and Date
      2008-07-04
    • Related Report
      2008 Annual Research Report
  • [Book] エナメル質比較発生学懇話会著,アメロジェニン蛋白の分布から見たエナメル質の系統発生エナメル質形成、構造、遺伝、再生、起源と進化2009

    • Author(s)
      石山巳喜夫,三上正人,今井あかね,下村浩巳
    • Publisher
      わかば出版
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ngt.ndu.ac.jp/guide/kouza/kiso04.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://wwwsoc.nii.ac.jp/jbiochem/magazine/82-02-05.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ngt.ndu.ac.jp/guide/kouza/kiso04.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ngt.ndu.ac.jp/guide/kouza/kiso04.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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