Development of methodology for the hard tissue regeneration using dental pulp cells
| Project/Area Number |
20592192
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
FUKASAWA Kayoko Matsumoto Dental University, 大学院・歯学独立研究科, 准教授 (60064698)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
UEHARA Shunsuke 松本歯科大学, 歯学部, 助教 (90434480)
NAKAMURA Midori 松本歯科大学, 歯学部, 講師 (90278177)
UDAGAWA Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 歯髄細胞 / 再生医学 / 石灰化機構 / 硬組織再生 / 石灰化 / 象牙質 / セメント質 / 骨芽細胞 / BMP |
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| Research Abstract |
We characterized dental pulp cells in comparison with osteoblasts and explored the mechanisms of calcification. We found that densely cultured dental pulp cells exhibited stronger ECM (extracellular matrix) calcification activity than osteoblasts. A microarray analysis showed the overall gene expression in dental pulp cells to be similar to that in osteoblasts, though dental pulp cells expressed higher level of annexin A8. Then, we examined the effect of overexpression and knockdown of annexin A8 on ECM calcification in osteoblasts and dental pulp cells. Overexpression of annexin A8 remarkably induced ECM calcification in osteoblasts. In contrast, knockdown of annexin A8 in dental pulp cells suppressed ECM calcification. We transplanted three-dimensional culture of dental pulp cells into immunocompromised mice and found that the grafts generated bone in which hematopoiesis was carried out. Taken together, these results suggest that transplanted dental pulp cells exert bone regenerative activity through the expression of annexin A8.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Alisol-B, a novel phyto-steroid, suppresses the RANKL-induced osteoclast formation and prevents bone loss in mice.2010
Author(s)
Lee JW, Kobayashi Y, Nakamichi Y, Udagawa N, Takahashi N, Im NK, Seo HJ, Jeon WB, Yonezawa T, Cha BY, Woo JT
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Journal Title
Biochem Pharmacol 80
Pages: 352-361
Related Report
Peer Reviewed
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[Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast formation and function in different manners.2009
Author(s)
Takahashi M, Mizoguchi T, Uehara S, Nakamichi Y, Yang S, Naramoto H, Yamashita T, Kobayashi Y, Furusawa K, Udagawa N, Uematsu T, Takahashi N
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Journal Title
J Bone Miner Metab 27
Pages: 24-35
NAID
Related Report
Peer Reviewed
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[Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo.2009
Author(s)
Mizoguchi T, Muto A, Udagawa N, Arai A, Yamashita T, Hosoya A, Ninomiya T, Nakamura H, Yamamoto Y, Kinugawa S, Nakamura M, Nakamichi Y, Kobayashi Y, Nagasawa S, Oda K, Tanaka H, Tagaya M, Penninger JM, Ito M, Takahashi N
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Journal Title
J Cell Biol 184
Pages: 541-554
Related Report
Peer Reviewed
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