| Project/Area Number |
20592192
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
FUKASAWA Kayoko Matsumoto Dental University, 大学院・歯学独立研究科, 准教授 (60064698)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
UEHARA Shunsuke 松本歯科大学, 歯学部, 助教 (90434480)
NAKAMURA Midori 松本歯科大学, 歯学部, 講師 (90278177)
UDAGAWA Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 歯髄細胞 / 再生医学 / 石灰化機構 / 硬組織再生 / 石灰化 / 象牙質 / セメント質 / 骨芽細胞 / BMP |
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| Research Abstract |
We characterized dental pulp cells in comparison with osteoblasts and explored the mechanisms of calcification. We found that densely cultured dental pulp cells exhibited stronger ECM (extracellular matrix) calcification activity than osteoblasts. A microarray analysis showed the overall gene expression in dental pulp cells to be similar to that in osteoblasts, though dental pulp cells expressed higher level of annexin A8. Then, we examined the effect of overexpression and knockdown of annexin A8 on ECM calcification in osteoblasts and dental pulp cells. Overexpression of annexin A8 remarkably induced ECM calcification in osteoblasts. In contrast, knockdown of annexin A8 in dental pulp cells suppressed ECM calcification. We transplanted three-dimensional culture of dental pulp cells into immunocompromised mice and found that the grafts generated bone in which hematopoiesis was carried out. Taken together, these results suggest that transplanted dental pulp cells exert bone regenerative activity through the expression of annexin A8.
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