• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of invasion/metastasis inhibition therapy against oral squamous cell carcinomas : a basic study.

Research Project

Project/Area Number 20592205
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionOita University

Principal Investigator

KAWANO Kenji  大分大学, 医学部, 教授 (50214664)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords口腔扁平上皮癌 / 浸潤 / 転移 / 接着分子 / アノイキス / anoikis / 口腔扁平上癌
Research Abstract

Multicellular aggregates of MOK205, an oral squamous carcinoma cell line, transformed to monolayer colonies on laminin 5 and type 1 collagen substrates, and then showed cell dispersion only on laminin 5. This phenomenon was triggered by the increased motility and disruption of E-cadherin-mediated intercellular adhesion via the activation of integrin receptor by laminin 5. When PP1, a specific inhibitor of the Src family, was added to the cell scattering model of MOK205, cell dispersion was almost completely blocked, suggesting that Src was involved in the interaction between integrin and cadherin. In addition, fluorescence microscopic observation revealed that phospho-tyrosine 215-Src and phospho-tyrosine 529-Src were localized along the cell membrane of the intercellular adhesion of MOK205 immediately before cells started dispersion on laminin 5. Taken together, these results suggested that Src could be a target of the invasion/metastasis inhibition therapy against oral squamous cell carcinomas.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi