| Project/Area Number |
20592220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO Takuichi 東北大学, 大学院・歯学研究科, 講師 (10303132)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAUCHI Hidetoshi 東北大学, 大学院・歯学研究科, 教授 (70187425)
TAKAHASHI Nobuhiro 東北大学, 大学院・歯学研究科, 教授 (60183852)
YAMAKI Keiko 東北大学, 大学院・歯学研究科, 助教 (90182419)
MAYANAGI Gen 東北大学, 大学院・歯学研究科, 助教 (10451600)
MATSUYAMA Junko 新潟大学, 医歯学系, 助教 (30293236)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯学 / 口腔細菌 / 根尖性歯周炎 / PCR / 16SrRNA / 16S rRNA |
|---|
| Research Abstract |
Development of novel microflora-profiling methods of infected root canals and their application to endodontic therapy The aim of this study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacterial identification, before and after root canal treatment. Samples from infected root canals before and after treatment were collected, followed by anaerobic culture. After incubation, CFUs were counted and isolated bacteria were identified by 16S rRNA gene sequencing. The findings of this study suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.
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