| Project/Area Number |
20592232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
KATAYAMA Tadashi Meikai University, 歯学部, 教授 (10105596)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Yukio 明海大学, 歯学部, 教授 (40207931)
SUDA Tomoyo 明海大学, 歯学部, 助教 (60226579)
ISHIHARA Satiyo 明海大学, 歯学部, 講師 (10223017)
IWASAKA Kensuke 明海大学, 歯学部, 講師 (60424058)
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| Co-Investigator(Renkei-kenkyūsha) |
KIKUCHI Hirotaka 明海大学, 歯学部, 講師 (70234193)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医学 / 歯学 / 歯髄 / 生体材料 / PRP |
|---|
| Research Abstract |
The efficacy of the extract of washed platelet (WPLT) as a stimulator of the proliferation and the calcification of human dental pulp stem cells was investigated. The growth of pulp cells was weakly stimulated by treatment with platelet-rich plasma (PRP), while it was more effectively stimulated by WPLT. Moreover, platelet-poor plasma (PPP) dose-dependently inhibited the growth of cultured human dental pulp cells. WPLT stimulated the calcification induced by β-glycerophosphate and ascorbic acid in the pulp cells, although WPLT alone was ineffective. WPLT significantly stimulated the mRNA expression of bone Gla protein (BGP), but not that of bone sialoprotein (BSP) or dentin sialoprotein (DSP), β-glycerophosphate and ascorbic acid stimulated both BGP and DSP, and addition of WPLT with BGP and Aa did not produce any addition or ayregic effect of the mRNA expression by β-glycerophosphate and ascorbic acid. These results suggest that WPLT is a potent stimulator of human dental pulp cells, and play a significant role in bone calcification.
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