| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
The gene expression of amelogenin in several mouse tissues were evaluated in this study. The samples were chosen from embryonic 9day, 18day mouse tissue mix cDNA, 8week mouse tissues mix cDNA and several each target tissues cDNA. The target tissues for this study were selected 16 tissues (brain, heart, lung, liver, stomach, uterus, placenta, kidney, pancreas, testine, muscle, skin, small intestine, large intestine, spleen, and ovary)from 8week old mouse body. The polymerase chain reaction(PCR)was used to amplify several size of amelogenin using a part of exon6 to exon7(small size of PCR amplification : 226bp).The result of PCR amplification showed the positive react of gene expression at 8week mouse tissues mix cDNA and 18day mouse tissue mix cDNA. However none of gene expression was detected by embryonic 9 day mouse tissues mix cDNA. The result of the PCR amplification for 16tissues was obtained amelogenin gene expression in 14 tissues excluded stomach and pancreas. These results suggested that amelogenin genes are expressed in various tissues and this expression is not only restricted to development stage that also expressed in adult mouse. These results suggested that amelogenin might play some homeostatic role in various tissues of the body.
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