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Regeneration of dentin by human skeletal muscle stem cells and developments of new therapies using stem cell injections.

Research Project

Project/Area Number 20592244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

NAKATA Kazuhiko  Aichi Gakuin University, 歯学部, 講師 (70261013)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hiroshi  愛知学院大学, 歯学部, 教授 (40064878)
OZEKI Nobuaki  愛知学院大学, 歯学部, 講師 (70469005)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords体性幹細胞 / ヒト骨格筋幹細胞(h-SMSCs) / 象牙芽細胞 / 象牙質再生 / 骨格筋幹細胞 / 幹細胞導入治療法
Research Abstract

We have examined the mechanism of human skeletal muscle stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and Enamelysin; both markers of odontoblast differentiation. We also found significant integrin α1β1, αVβ1 and αVβ3 expression with increased adhesion and motility on collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing. Moreover, differentiated cells was injected showed DSP and osteo-dentin in rats.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (6 results)

All 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] α7 integrin陽性骨格筋幹細胞の象牙質分化能2009

    • Author(s)
      尾関伸明、川合里絵, 田中毅、石塚恭子、中田和彦、中村洋
    • Journal Title

      日本歯科保存学雑誌 52巻(4)

      Pages: 319-329

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] α7integrin 陽性ヒト骨格筋幹細胞の象牙質分化能2009

    • Author(s)
      尾関伸明
    • Journal Title

      日本歯科保存学雑誌 52巻(4)

      Pages: 319-329

    • NAID

      10019830849

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Odontogenic potential of α7 integrin-positive human skeletal muscle stem cells2010

    • Author(s)
      尾関伸明, et al.
    • Organizer
      International Associaton for Dental Research
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Odontogenic potential of α7 integrin-positive human skeletal muscle stem cells2010

    • Author(s)
      Nobuaki Ozeki
    • Organizer
      88^<th> General Session & Exhibition of the IADR
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] α7 integrin陽性ヒト骨格筋幹細胞の象牙質分化能2009

    • Author(s)
      尾関伸明, 他
    • Organizer
      第75回愛知学院大学歯学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-12-06
    • Related Report
      2010 Final Research Report
  • [Presentation] α7integrin 陽性ヒト骨格筋幹細胞の象牙質分化能2009

    • Author(s)
      尾関伸明
    • Organizer
      愛知学院大学歯学会
    • Place of Presentation
      愛知学院大学歯学部楠元学舍
    • Year and Date
      2009-12-06
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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