Development of sponge scaffold and osteogenic promoting factors in the scaffold for dentine-pulp complex regeneration
| Project/Area Number |
20592246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
YOSHIKAWA Masataka Osaka Dental University, 歯学部, 講師 (70148451)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 歯内療法学 / ハイドロキシアパタイト / アルギン酸 / ゼラチン / ポリビニルアルコール / セルロース / スポンジ担体 / 硬組織再生 / 骨髄細胞 / 組織工学 / 骨髄幹細胞 / デキストラン / 担体 / スポンジ / ヒアルロン酸 |
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| Research Abstract |
The use of porous ceramics is desirable as a scaffold for osteogenesis by undifferentiated mesenchymal stem cells. In the scaffold, an active proliferation and differentiation to hard tissue formation cells was expected for the cells. Therefore an effective factor to differentiate the cells was examined. As a result, it was recognized in hyaluronic acid, laminin and leucine to promote the cell proliferation and differentiation effectively. And it was recognized that the dextran was effective for the differentiation of the undifferentiated mesenchymal cells. The ceramics scaffold received the bone marrow stem cells in the pores. However, it was found that the attachment of the bone marrow stem cells in the sponge with fibrous structure was impossible because of the fibrous structure. Coating of the dextran to the sponge gave width to the fiber, and attachment of bone marrow stem cells would be possible. Oteogenesis in the sponge by bone marrow stem cells was induced in vitro and in vivo.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article]2010
Author(s)
Yoshikawa M, Tsuji N, Kakigi H, Yabuuchi T, Shimomura Y, Hayashi H, Ohgushi H
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Journal Title
Journal of Biomedical Science and Engineering 3
Pages: 751-757
Related Report
Peer Reviewed
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