Development osseoinductive nano-textured implant and analysis of its gene network

• Project/Area Number: 20592289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Nihon University
• Principal Investigator: SATOH Yoshinori Nihon University, 歯学部, 教授 (70060051)
• Co-Investigator(Kenkyū-buntansha): INAGE Toshihiko 日本大学, 歯学部, 准教授 (90096769) ; KUWATA Fumiyuki 日本大学, 歯学部, 教授 (60120440)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯学 / ナノ材料 / シグナル伝達 / 蛋白質 / 核酸 / Implant / bone formation / Implant surface / bone-implant interface / 骨誘導 / チタン / インプラント

Research Abstract

A novel nano-surfaced titanium has been generated by irradiated with Femto-sec laser and demonstrate stimulation of proliferation and differentiation of rat bone stroma cells. Numbers of cultured cell were counted and expressions of collagen and osteocalcin were studied in vitro. The surface roughness was increased by laser irradiation. In the culture with nano-surfaced Ti, the numbers of cultured were increased than those of machined surface at 7 day and at 10 day after the culture. The number of osteocalcin-positive cell increased than that of collagen-positive cell by the immunohistochemistry at 10 day after the culture.

Research Products

• [Journal Article] レーザー加工を用いた医療用ナノ表面チタンの骨芽細胞の分化に関する影響 (2010)
  • Author(s): 稲毛稔彦,佐藤吉則,稲毛寛彦,藤田哲夫,桑田文幸
  • Journal Title: 日大歯学 84巻 Pages: 10-15
  • Peer Reviewed
• [Journal Article] インプラント用チタン上で培養した骨芽細胞の分化に関する研究 (2010)
  • Author(s): 稲毛稔彦
  • Journal Title: 日大歯学 Volume: 84 Pages: 111-126
  • Peer Reviewed
• [Presentation] The regeneration of bone Via Nanoscopic Poss-collagen Nano-surface Ti Implant for bone (2010)
  • Author(s): T.Inage
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Nano-surfaced Biomimetic Ti Implant for Bone (2010)
  • Author(s): T.Inage, H.Inage, T.Fujita, Y.Satoh
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] Efficiency of Nano-surfaced Ti Implant for bone formation (2009)
  • Author(s): T.Inage, H.Inage, T.Fujita, F.Kuwata, Y.Satoh
  • Organizer: American Dental Association2009年次総会
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2009-10-02
• [Presentation] Efficiency of Nano-surfaced Ti Implant for Bone Formation (2009)
  • Author(s): T.Inage, H.Inage, T.Fujita, F.Kuwata, Y.satoh
  • Organizer: American dental association 2009 Annual Session
  • Place of Presentation: Hawaii Convention Center(USA)
  • Year and Date: 2009-10-02