FGFR2b signaling controls the differentiation and function of Dental Pulp Stem Cells

• Project/Area Number: 20592294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KUREMOTO Koh-Ichi Osaka Dental University, 歯学部, 助教 (90319583)
• Co-Investigator(Kenkyū-buntansha): MAEDA Teruta 大阪歯科大学, 歯学部附属病院, 教授 (10103110)
• Co-Investigator(Renkei-kenkyūsha): YAMAZA Takayoshi 九州大学, 歯学部, 助教 (80304814)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: FGF / 歯髄幹細胞 / 象牙質再生

Research Abstract

Rodent incisors regenerate throughout the animal lifetime as a result of the continuous deposition of enamel, the hardest component of the tooth, by ameloblasts. Putative dental epithelial stem cells (DESCs) reside at the base of the incisors in a structure called the cervical loop (CL). The DESCs can self renew and give rise to ameloblast progenitor cells (APCs), which proliferate and differentiate into ameloblasts producing enamel. In this study, we explored the role of Fibroblast Growth Factor Receptor 2b (FGFR2b) signaling during incisor homeostasis in the adult mice. FGFR2b signaling plays a critical role in the regenerative capacity of adult incisors by controlling the proliferation of transit amplifying APCs, but not by regulating the survival of DESCs.

Research Products

• [Journal Article] Signaling by FGFR2b controls the regenerative capacity of adult mouse incisors (2010)
  • Author(s): Parsa S, Kuremoto K, Seidel K, Tabitatai Irani R, MacKenzie B, Yamaza T, Akiyama K, Branch J, Koh C, Al Alam D, Klein O.D., Bellusci S
  • Journal Title: Development 137巻 Pages: 3743-3752
  • Peer Reviewed
• [Journal Article] Signaling by FGFR2b controls the regenerative capacity of adult mouseincisors. (2010)
  • Author(s): Parsa S, Kuremoto K, Seidel K, 他
  • Journal Title: Development Volume: 137 Pages: 3743-3752
  • Peer Reviewed
• [Presentation] 線維芽細胞増殖因子が口腔上皮由来幹細胞の機能と分化を制御する (2011)
  • Author(s): 呉本晃一, 他
  • Organizer: 日本補綴歯科学会第120回記念学術大会
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2011-05-22
• [Presentation] FGFR2b signaling and ameloblast stem cells in mouse incisor regeneration (2010)
  • Author(s): 呉本晃一, 他
  • Organizer: 88th General Session & Exibition of the Internatinal Assosiacin for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona(Spain)
  • Year and Date: 2010-07-17
• [Presentation] FGFR2b signaling and ameloblast stem cells in mouse incisor regeneration. (2010)
  • Author(s): Kuremoto K, 他
  • Organizer: 88th General Session & Exibition of the Internatinal Assosiacin for Dental Research
  • Place of Presentation: Barcerona, Spain
  • Year and Date: 2010-07-17
• [Presentation] Fibroblast Growth Factor 10-mediated Delay in Bladder Wound Healing in Inducible Transgenic Mice (2009)
  • Author(s): Koh-ichi Kuremoto
  • Organizer: American Urological Association Annual Meeting 2009
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-04-26
• [Presentation] Identifying the Role of Fibroblast Growth Factor Signaling in Bladder Urothelium using Inducible Transgenic Mice (2008)
  • Author(s): Koh-ichi Kuremoto
  • Organizer: American College of Surgeons 94th Annual Clinical Congress
  • Place of Presentation: San Francisco, USA