Possibility of periodontal regeneration using dental pulp stem cells

• Project/Area Number: 20592315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tsurumi University
• Principal Investigator: GOMI Kazuhiro Tsurumi University, 歯学部, 准教授 (10178460)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 歯髄幹細胞 / 歯周組織再生 / CD-44 / CD-90 / b-FGF / 免疫不全ラット / 結合組織性付着 / CD90 / トリチウム(3H)サイミジン / SRTO-1 / FGF-2 / 組織工学 / CD44

Research Abstract

The purpose of this study was examined the localization of pulp stem cells in the pulp, which is able to obtain without tooth extraction, and evaluated the growth of stem cells and the surface antigen with FGF addition culture. Also, we were evaluated the regenerated tissue using pulp stem cells in artificially created periodontal bone defect. As a result, (1) the pulp stem cells, which have high proliferation rate, were located in the center of coronal pulp chamber. (2) As for the cellular surface antigen, which performed an amplification culture by FGF, CD34 and CD45 were negative, and it was shown that CD44 and CD90 were positive. (3) After implantation of pulp stem cells in artificial periodontal bone defect, connective tissue attachment was found in the experimental group, but the alveolar bone regeneration was not found. In this study, we implanted human pulp stem cells to rat ; it was thought that there were some immune problems. However, it was thought that pulp stem cells has a possibility for periodontal regeneration.

Research Products

• [Journal Article] Microbicidal and cytotoxic effects of functional water in vitro. (2010)
  • Author(s): Gomi K, Makino T, Suzuki S, Hasegawa M, Maeda N, Arai T
  • Journal Title: Quintessence Int. 41 Pages: 166-172
  • Peer Reviewed
• [Journal Article] Cytodifferentiation activity of synthetic human enamel sheath proteinpeptides. (2010)
  • Author(s): Kakegawa A, Oida S, Gomi K, Nagano T, Yamakoshi Y, Fukui T, Kanazashi M, Arai T, Fukae M
  • Journal Title: J Periodont Res. 45 Pages: 643-649
  • NAID: 130004570949
  • Peer Reviewed
• [Journal Article] Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells. (2009)
  • Author(s): Morito A, Kida Y, Suzuki K, Inoue K, Kuroda N, Gomi K, Arai T, Sato T
  • Journal Title: Arch Histol Cytol. 72 Pages: 51-64
  • NAID: 10025868067
  • Peer Reviewed
• [Journal Article] Mmp-20 and Klk4 Cleavage Site Preferences for Amelogenin Sequences. (2009)
  • Author(s): T. Nagano, A. Kakegawa, Y. Yamakoshi, S. Tsuchiya, J.C.-C. Hu, K. Gomi, T. Arai, J.D. Bartlett, J.P. Simmer
  • Journal Title: J Dent Res. 88 Pages: 823-828
  • Peer Reviewed
• [Journal Article] One-stage full-mouth versus partial-mouth scaling and root planning during the effective half-life of systemically administered azithromycin. (2009)
  • Author(s): Yashima A, Gomi K, Maeda N, Arai T
  • Journal Title: J Periodontol. 80 Pages: 1406-1413
  • Peer Reviewed
• [Journal Article] Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells (2009)
  • Author(s): Morito A, Kida Y, Suzuk K, Inoue K, Kuroda N, Gomi K, Arai T, Sato T
  • Journal Title: Arch Histol Cytol 72 Pages: 51-64
  • NAID: 10025868067
  • Peer Reviewed
• [Journal Article] Degradation of noncollagenous components by neutrophil elastase reduces the mechanical strength of rat periodontal ligament. (2008)
  • Author(s): Ujiie Y, Shimada A, Komatsu K, Gomi K, Oida S, Arai T, Fukae M
  • Journal Title: J Periodont Res. 43 Pages: 22-31
  • Peer Reviewed
• [Presentation] アジスロマイシンを用いた歯周抗菌療法の多施設研究 (2010)
  • Author(s): 新田浩,五味一博,新井高,前田伸子,福田光男,野口俊英,野杁由一郎,恵比須繁之,島田靖子,吉江弘正
  • Organizer: 第53回日本歯周病学会秋季学術大会
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2010-09-19
• [Presentation] Effect of azithromycin to gingival and periodontal ligament fibroblasts. (2010)
  • Author(s): Gomi K, Yamaguchi T, Nagano T, Momoi Y, Arai T
  • Organizer: The 96^<th> Annual Meeting of American Academy of Periodontolog y in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Hawaii Convention Center, Honolulu, HI
• [Presentation] インプラント周囲炎に対するfull-mouth SRPによる細菌叢の変化 (2009)
  • Author(s): 牧野智彦,大島朋子,五味一博,川崎文嗣,小川比佐誌,八島章博,前田伸子,新井高
  • Organizer: 第52回日本歯周病学会秋季学術大会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2009-10-11
• [Presentation] full-mouth SRPと歯周外科処置を併用した侵襲性歯周炎の一症例 (2009)
  • Author(s): 八島章博,五味一博,新井高
  • Organizer: 第52回日本歯周病学会秋季学術大会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2009-10-11
• [Presentation] The effects of full mouth SRP conjunction with Azithromycin for peri-implantitis. (2009)
  • Author(s): Makino T, Gomi K, Yashima A, Kawasaki F, Oshima T, Maeda N, Arai T
  • Organizer: The 6^<th> Annual Meeting of Asian Pacific Society of Periodontology
  • Place of Presentation: Cariton Hotel, Singapore
• [Presentation] One-stage full- versus partial-mouth scaling and root planing under vali dity period of systemically administ rated azithromycin. (2009)
  • Author(s): Yashima A, Gomi K, Maeda N, Arai T
  • Organizer: EUROPERIO6
  • Place of Presentation: Stockholm International Fairs and Congress Centre, Stockholm, Sweden
• [Presentation] 抗菌薬併用によるfull-mouth SRPにおける1回法vs.分割法の比較 (2009)
  • Author(s): 八島章博,五味一博,牧野智彦,近内理代,前田伸子,新井高
  • Organizer: 第52回日本歯周病学会春季学術大会
  • Place of Presentation: 岡山コンベンショナルセンター
• [Presentation] インプラント周囲炎に対するfull-mouth disinfectionの細菌学的効果 (2008)
  • Author(s): 牧野智彦,小川比佐誌,川崎文嗣,大島朋子,五味一博,前田伸子,新井高
  • Organizer: 第51回日本歯周病学会秋季学術大会
  • Place of Presentation: 四日市市文化会館
  • Year and Date: 2008-10-19
• [Presentation] アジスロマイシンを用いた歯周抗菌療法の多施設研究-中間報告- (2008)
  • Author(s): 五味一博,新井高,前田伸子,野杁由一郎,恵比須繁之,小田茂,和泉雄一,新田浩,山本幸司,吉江弘正,福田光男,野口俊英
  • Organizer: 第51回日本歯周病学会秋季学術大会
  • Place of Presentation: 四日市市文化会館
  • Year and Date: 2008-10-19
• [Book] 薬'10/'11歯科疾患名から治療薬と処方例がすぐわかる本 (2010)
  • Author(s): 朝波惣一郎、王宝禮編集,五味一博
  • Publisher: クインテッセンス出版
• [Book] Periodontal Therapy Basic and Clinical Practices 3^<rd> Edition (2009)
  • Author(s): 新井高,五味一博,八島章博, 他
  • Total Pages: 255
  • Publisher: 永末書店
• [Book] 歯周病予防のストラテジー (2009)
  • Author(s): 吉江弘正、宮田隆編,五味一博
  • Publisher: 医歯薬出版
• [Book] 薬 (2008)
  • Author(s): 朝波惣一郎、王宝禮編集,五味一博
  • Publisher: クインテッセンス出版