Clarification of interaction between β-catenin and PTH in osteogenesis for craniofacial bone regeneration

• Project/Area Number: 20592320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: KANNO Yuki The University of Tokyo, 医学部附属病院, 助教 (80451813)
• Co-Investigator(Kenkyū-buntansha): OGASAWARA Toru 東京大学, 医学部附属病院, 講師 (20359623) ; YANO Fumiko 東京大学, 大学院・医学系研究科, 特任助教 (80529040) ; 近津 大地 東京大学, 医学部附属病院, 助教 (30343122) ; 大橋 克巳 東京大学, 医学部・附属病院, 助教 (60233235)
• Co-Investigator(Renkei-kenkyūsha): OGATA Naoshi 東京大学, 医学部附属病院, 講師 (10361495)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 顎顔面 / 骨再生 / PTHシグナル / β-catenin / PTH / PTH/PTHrP受容体 / PTHrP受容体 / βカテニン / 骨形成 / 顎顔面骨再生 / G蛋白 / Wntシグナル

Research Abstract

The purpose of this study is to clarify regulatory mechanism of bone metabolism and its effect via interaction between PTH signaling, that has anabolic effect in bone, and β-catenin binding to PTH/PTHrP receptors. By elucidating osteogenic function via the interaction, we aim to develop PTH-related osteogenesis-accelerating drugs that are more effective with less side-effect.

Research Products

• [Journal Article] Enhancement of boneformation ex vivo and in vivo by a helioxanthin-derivative. (2010)
  • Author(s): Nakajima K, Komiyama Y, Hojo H, Ohba S, Yano F, Nishikawa N,Ihara S, Aburatani H, Takato T, Chung UI
  • Journal Title: Biochem Biophys Res Commun. 395(4) Pages: 502-508
  • Peer Reviewed
• [Journal Article] Trehalose inhibits oral dryness by protecting the cell membrane. (2010)
  • Author(s): Mori Y, Yano F, Shimohata N, Suzuki S, Chung UI, Takato T.
  • Journal Title: Int J Oral Maxillofac Surg. 39(9) Pages: 916-921
  • Peer Reviewed
• [Journal Article] Tumor-induced osteomalacia associated with a maxillofacial tumor producing fibroblast growth factor 23 : report of a case and review of the literature. (2010)
  • Author(s): Mori Y, Ogasawara T, Motoi T, Shimizu Y, Chikazu D, Tamura K, Fukumoto S, Takato T.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 109(3) Pages: 57-63
  • Peer Reviewed
• [Journal Article] A bone harvesting method involving a surgical template prepared using a three-dimensional model (2010)
  • Author(s): Saijo H, Kanno Y, Mori Y, Chikazu D, Iino M, Takato T.
  • Journal Title: Asian.J. Oral.Maxillofac.Surg. 22 Pages: 20-22
  • Peer Reviewed
• [Journal Article] Evaluation and analysis of bone formation at palate of cleft lip and palate patients after palatoplasty based on computed tomographic scanning and three dimensional data. (2010)
  • Author(s): Saijo H, Mori Y , Kanno Y, Chikazu D, Fujihara H, Ohkubo K, Hikiji H, Iino S, Yonehara Y, Takato T
  • Journal Title: Journal of Plastic Surgery & Hand Surgery 44 Pages: 21-25
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of endochondral ossification by HIF-2alpha during skeletal growth and osteoarthritis development. (2010)
  • Author(s): Saito T, Fukai A, Mabuchi A, Ikeda T, Yano F, Ohba S, Nishida N, Akune T, Yoshimura N, Nakagawa T, Nakamura K, Tokunaga K, Chung UI, Kawaguchi H.
  • Journal Title: Nat Med. 16(6) Pages: 678-686
  • Peer Reviewed
• [Journal Article] Runx1 and Runx2 cooperate during sternal morphogenesis. (2010)
  • Author(s): Kimura A, Inose H, Yano F, Fujita K, Ikeda T, Sato S, Iwasaki M, Jinno T, Ae K, Fukumoto S, Takeuchi Y, Itoh H, Imamura T, Kawaguchi H, Chung UI, Martin JF, Iseki S, Shinomiya K, Takeda S.
  • Journal Title: Development. 137(7) Pages: 1159-67
  • Peer Reviewed
• [Journal Article] Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system. (2010)
  • Author(s): Hojo H, Yano F, Ohba S, Igawa K, Nakajima K, Komiyama Y, Kan A, Ikeda T, Yonezawa T, Woo JT, Takato T, Nakamura K, Kawaguchi H, Chung UI.
  • Journal Title: J Bone Miner Metab. 28(6) Pages: 627-33
  • NAID: 10027845798
  • Peer Reviewed
• [Journal Article] Akt1 in murine chondrocytes controls cartilage calcification during endochondral ossification under physiologic and pathologic conditions. (2010)
  • Author(s): Fukai A, Kawamura N, Saito T, Oshima Y, Ikeda T, Kugimiya F, Higashikawa A, Yano F, Ogata N, Nakamura K, Chung UI, Kawaguchi H.
  • Journal Title: Arthritis Rheum. 62(3) Pages: 826-836
  • Peer Reviewed
• [Journal Article] A bone harvesting method involving a surgical template prepared using a three-dimensional model (2010)
  • Author(s): Saijo H, et al.
  • Journal Title: Asian Journal of Oral and Maxillofacial Surgery Volume: 22 Pages: 20-22
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of endochondral ossification by HIF-2alpha during skeletal growth and osteoarthritis development. (2010)
  • Author(s): Saito T, et al.
  • Journal Title: Nat Med. Volume: 16 Pages: 678-686
  • Peer Reviewed
• [Journal Article] Coordination of chondrogenesis and osteogenesis by hypertrophic chondrocytes in endochondral bone development. (2010)
  • Author(s): Hojo H, et al.
  • Journal Title: J Bone Miner Metab. Volume: 28 Pages: 489-502
  • Peer Reviewed
• [Journal Article] Transforming Growth Factor-β1 in Combination with Fibroblast Growth Factor-2 and Insulin-like Growth Factor-I for Chondrocyte Proliferation Culture and Cartilage Regenerative Medicine. (2009)
  • Author(s): Ogasawara T, Ohba S, Fujihara Y, Takahashi T, Liu G, Chikazu D, Suenaga H, Chung UI, Yoda T, Mori Y, Susami T, Takato T, Hoshi K.
  • Journal Title: Asian J Oral Maxillofac Surg. 21 Pages: 18-26
  • Peer Reviewed
• [Journal Article] Three-dimensional shape control of implant devices (2009)
  • Author(s): Chung U-I, et al.
  • Journal Title: The Handbook of Intelligent Scaffold for Regenerative Medicine (In press)
  • Peer Reviewed
• [Journal Article] Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology (2009)
  • Author(s): Saijo H, et al.
  • Journal Title: J Artif Organs 12 Pages: 200-205
  • Peer Reviewed
• [Journal Article] A bone harvesting method involving a surgical template prepared using a three-dimensional model (2009)
  • Author(s): Saijo H, et al.
  • Journal Title: Asian Journal of Oral and Maxillofacial Surgery (In press)
  • Peer Reviewed
• [Journal Article] Development of high-throughput screening system for osteogenicdrugs using a cell-based sensor. (2008)
  • Author(s): Hojo H, Igawa K, Ohba S, Yano F, Nakajima K, Komiyama Y,Ikeda T, Lichtler AC, Woo JT, Yonezawa T, Takato T, Chung UI.
  • Journal Title: Biochem Biophys Res Commun. 376(2) Pages: 375-379
  • Peer Reviewed
• [Journal Article] A case of tumoural calcinosis in the temporomandibular joint associated with systemic sclerosis. (2008)
  • Author(s): Daichi Chikazu et al.,
  • Journal Title: Int J Oral Maxillofac Surg 37 Pages: 190-193
  • Peer Reviewed
• [Journal Article] Effects of transforming growth factor (TGF)-β1 in combination with fibroblast growth factor (FGF)-2 and insulin-like growth factor (IGF)-I on chondrocytes proliferation culture for the cartilage regenerative medicine (2008)
  • Author(s): Toru Ogasawara et al.,
  • Journal Title: Asian J Oral Maxillofac Surg (In press)
  • Peer Reviewed
• [Journal Article] Patchedl haploinsufficiency increases adult bone mass and modulates Gli3 repressor activity (2008)
  • Author(s): Shinsuke Ohba et al.,
  • Journal Title: Dev Cell 14 Pages: 689-699
  • Peer Reviewed
• [Presentation] A novel method for designing and fabricating custom-made artificial bones. (2010)
  • Author(s): Kanno Y, Takato T, Saijo H, Igawa K, Seto I, Mori Y, Ohkubo K, Sasaki N, Suzuki S, Chung UI.
  • Organizer: Bernd-Spiessl-Symposium 2010
  • Place of Presentation: Basel,Switzerland.
  • Year and Date: 2010-06-18
• [Presentation] A novel method for designing and fabricating custom-made artificial bones. (2010)
  • Author(s): Yuki Kanno, et al.
  • Organizer: Bernd-Spiessl Symposium
  • Place of Presentation: バーゼル大学(スイス、バーゼル)
  • Year and Date: 2010-06-18
• [Presentation] カスタムメイド人工骨移植後の骨癒合状態の評価 (2010)
  • Author(s): 菅野勇樹,西條英人,井川和代,斉藤健太郎,瀬戸一郎,森良之,高戸毅
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 幕張メッセリンク,千葉
• [Presentation] 両側顎関節強直症に対し、人工下顎頭を応用した1例 (2010)
  • Author(s): 菅野勇樹、西條英人、斎藤健太郎、宇波雅人、大久保和美、近津大地、森良之、高戸毅
  • Organizer: 第64回NPO法人日本口腔科学会学術大会
  • Place of Presentation: 札幌プリンスホテル,北海道
• [Presentation] カスタムメイド人工骨移植後の移植位置評価 (2009)
  • Author(s): 菅野勇樹, ら
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2009-10-09
• [Presentation] 進行性骨化性線維異形成症(FOP)患者に対する全身麻酔下での抜歯経験 (2009)
  • Author(s): 森良之,須佐美隆史,西條英人,菅野勇樹,近津大地,末永英之,杉山円,前田祐二郎,斎藤健太郎,飯野光喜,高戸毅
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター,北海道
• [Presentation] カスタムメイド人工骨移植後の移植位置評価 (2009)
  • Author(s): 菅野勇樹,西條英人,井川和代,大久保和美,近津大地,森良之,飯野光喜,鄭雄一,高戸毅
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター,北海道
• [Presentation] βカテニンは軟骨細胞のPTH/PTHrP受容体の細胞内ドメインに直接結合して肥大分化を制御する (2009)
  • Author(s): 矢野文子,鄭雄一,池田敏之,斎藤琢,高戸毅,中村耕三,川口浩,緒方直史
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場,大阪
• [Presentation] 細胞周期制御を利用した骨粗鬆症治療法開発のための基礎的検討 (2009)
  • Author(s): 小笠原徹,高戸毅
  • Organizer: 第9回日本抗加齢医学会総会
  • Place of Presentation: ホテル日航東京,東京
• [Presentation] 上顎洞底挙上術時に洞粘膜の穿孔を合併した1例 (2008)
  • Author(s): 菅野勇樹,西條英人,藤原久子,斎藤健太郎,田中庸子,杉山円,前田祐二郎,飯野光喜,高戸毅
  • Organizer: 第12回日本顎顔面インプラント学会総会・学術集会
  • Place of Presentation: 日本歯科大学,東京
• [Presentation] GαqシグナルはPKCδを介してPTHの骨同化作用を抑制する (2008)
  • Author(s): 緒方直史,矢野文子,鄭雄一,中村耕三,川口浩
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場,大阪
• [Presentation] 新規低分子化合物チエノインダゾール誘導体はRunx1とSox trioを介して永久軟骨再生を誘導する (2008)
  • Author(s): 矢野文子,池田敏之,斎藤琢,緒方直史,木村文子,竹田秀,中村耕三,高戸毅,川口浩,鄭雄一
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場,大阪
• [Presentation] β-catenin Directly Binds to a Specific Intracellular Domain of PTH/PTHrP Receptor and Regulates the Signaling in Chondrocytes during Endochondral Ossification. (2008)
  • Author(s): Yano F, Chung UI, Ikeda Y, Kawasaki Y, Kawaguchi H, Tawfeek H, Mahon M.J, Segre G.V, Ogata N
  • Organizer: 30th annual meeting of the American Society for Bone and Mineral Research.
  • Place of Presentation: Convention Center Montreal, Quebec, Canada.
• [Presentation] Inhibitory role of Gαq/PKCαsignal in the bone anabolic action of PTH. (2008)
  • Author(s): Ogata N, Yano F, Chung UI, NWettschureck N., Offermanns S., Segre G.V., Nakamura K., Kawaguchi K.
  • Organizer: 30th annual meeting of the American Society for Bone and Mineral Research.
  • Place of Presentation: Convention Center Montreal, Quebec, Canada.
• [Presentation] 下顎骨半側切除後のプレート再建に三次元立体モデルによりシミュレーションを施行した1例 (2008)
  • Author(s): 菅野勇樹,西條英人,森良之,末永英之,山田陽子,前田祐二郎,近津大地,飯野光喜,高戸毅
  • Organizer: 第62回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡国際会議場,福岡
• [Presentation] Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology (2008)
  • Author(s): Yuki Kanno et al.,
  • Organizer: 8th Asia Congress on Oral and Maxillofacial Surgery
  • Place of Presentation: Bangkok
• [Book] 医師・歯科医師のための口腔診療必携 (2010)
  • Author(s): 小笠原徹, ら
  • Total Pages: 263
  • Publisher: 金原出版株式会社
• [Remarks] 東京大学医学部附属病院顎口腔外科・歯科矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks]
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks]
  • URL: http://plaza.umin.ac.jp/~oralsurg/
• [Remarks]
  • URL: http://plaza.umin.ac.jp/%7Eoralsurg/archive.html