The research for gingival fibroblasts character and its effect on the wound healing

• Project/Area Number: 20592326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagoya University
• Principal Investigator: EBISAWA Katsumi Nagoya University, 医学部附属病院, 病院助教 (20397459)
• Co-Investigator(Kenkyū-buntansha): NARITA Yuji 名古屋大学, 大学院・医学系研究科, 特任講師 (60378221) ; KAGAMI Hideaki 東京大学, 医科学研究所, 客員准教授 (80242866)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 創傷治癒 / 口腔粘膜由来線維芽細胞 / 再生医療 / 粘膜由来線維芽細胞

Research Abstract

From comparison of expression profiles of genes in dermal and gingival fibroblasts, only 5% showed a significant difference in the levels of expression. Hierarchical clustering of total genes showed that the difference between gingival and dermal fibroblasts was larger than the difference between ages or gender of patients. Wound closure was significantly accelerated in the wells treated with conditioned medium from gingival fibroblasts than in wells treated with dermal fibroblast conditioned medium, suggesting the presence of functional factor(s) in the conditioned medium that accelerate cell motility/growth of keratinocytes. These results should provide the possibility of effective treatment, which could be genetically designed to provide a higher therapeutic effect by selecting the origin of fibroblasts since gingival and dermal fibroblasts show some unique characteristics in their gene expression profiles.

Research Products

• [Journal Article] Gingival and dermal fibroblasts : Their similarities and differences revealed from gene expression. (2011)
  • Author(s): Ebisawa K, Kato R, Okada M, Sugimura T, Latif MA, Hori Y, Narita Y, Ueda M, Honda H, Kagami H.
  • Journal Title: J Biosci Bioeng. 111(3) Pages: 255-258
  • NAID: 110008593152
  • Peer Reviewed
• [Journal Article] Stem cells from human exfoliated deciduous teeth (SHED) enhance wound healing and the possibility of novel cell therapy. (2011)
  • Author(s): Nishino Y, Yamada Y, Ebisawa K, Nakamura S, Okabe K, Umemura E, Hara K, Ueda M
  • Journal Title: Cytotherapy.
  • Peer Reviewed
• [Journal Article] 自家培養線維芽細胞によるしわ、瘢痕の治療 (2011)
  • Author(s): 蛯沢克己、加藤竜司、亀井譲
  • Journal Title: PEPARS. 50 Pages: 22-31
• [Journal Article] Gingival and dermal fibroblasts : Their similarities and differences revealed from gene expression. (2011)
  • Author(s): Ebisawa K, Kato R, Okada M,Sugimura T, Latif MA, Hori Y, Narita Y, Ueda M, Honda H, Kaeami H
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 111(3) Pages: 255-258
  • NAID: 110008593152
  • Peer Reviewed
• [Journal Article] Stem cells from human exfoliated deciduous teeth(SHED) enhance wound healing and the possibility of novel cell therapy. (2011)
  • Author(s): Nishino Y, Yamada Y, Ebisawa K, Nakamura S, Okabe K, Umemura E, Hara K, Ueda M
  • Journal Title: Cytotherapy Volume: in press
  • Peer Reviewed
• [Journal Article] Human Deciduous Teeth Dental Pulp Cells (hDPC) with Basic Fibroblast Growth Factor (b-FGF) Enhance Wound Healing of Skin Defect (2011)
  • Author(s): Nishino Y, Ebisawa K, YamadaY, Okabe K, Kamei Y, Ueda M
  • Journal Title: Journal of Craniofacial Surgery Volume: in press
  • Peer Reviewed
• [Journal Article] 自家培養線維芽細胞によるしわ、癒痕の治療 (2011)
  • Author(s): 蛯沢克己、加藤竜司、亀井譲
  • Journal Title: PEPARS Volume: 50 Pages: 22-31
• [Journal Article] 老年医学領域における再生医療 (2010)
  • Author(s): 蛯沢克己、上田実
  • Journal Title: Geriatric Medicine 48 Pages: 83-83
• [Journal Article] (2010)
  • Author(s): 蛯沢克己、加藤竜司、岡田真衣、上田実
  • Journal Title: 抗加齢医療 その最前線の実際(新興医学出版)
• [Journal Article] 実用化は美容医療から (2009)
  • Author(s): 蛯沢克己、加藤竜司、上田実
  • Journal Title: Bio Industry 7月号 Pages: 40-46
• [Journal Article] 【アンチエイジング医学 基礎・臨床研究の進歩】臨床研究の進歩 老化抑制と健康寿命延長の方法論 アンチエイジング医学を担う再生医療 (2009)
  • Author(s): 蛯沢克己、各務秀明、加藤竜司、山田陽一、上田実
  • Journal Title: 日本臨床 67(7) Pages: 1402-1406
• [Journal Article] 実用化は再生医療から (2009)
  • Author(s): 蛯沢克己、各務秀明、加藤竜司、上田実
  • Journal Title: BIO INDUSTRY 7月号 Pages: 40-46
• [Journal Article] Human Deciduous Teeth Dental Pulp Cells (hDPC) with Basic Fibroblast Growth Factor (b-FGF) Enhance Wound Healing of Skin Defect.
  • Author(s): Nishino Y, Ebisawa K, Yamada Y, Okabe K, Kamei Y, Ueda M
  • Journal Title: J Craniofacial Surg. in press
  • Peer Reviewed
• [Presentation] Human Deciduous Teeth Dental Pulp Cells (hDPC) with Basic Fibroblast Growth Factor (b-FGF) Enhance Wound Healing of Skin Defect (2010)
  • Author(s): Ebisawa K, Nishino Y, Yamada Y, Okabe K, Ueda M, Kamei Y
  • Organizer: 第20回日中形成外科学会
  • Place of Presentation: 上海
  • Year and Date: 2010-08-27
• [Presentation] Human Deciduous Teeth Dental Pulp Cells(hDPC) with Basic Fibroblast Growth Factor (b-FGF) Enhance Wound Healing of Skin Defect (2010)
  • Author(s): Ebisawa K, Nishino Y, Yamada Y, Okabe K, Ueda M, Kamei Y
  • Organizer: 第20回日中形成外科
  • Place of Presentation: 上海
  • Year and Date: 2010-08-27
• [Presentation] 瘢痕に対する培養線維芽細胞注入 (2010)
  • Author(s): 蛯沢克己、加藤竜司、岡田真衣、上田実、亀井譲
  • Organizer: 第2回創傷外科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-07-30
• [Presentation] Clinical Experience of Cosmetic Regenerative Medicine for Facial Anti-Aging. (2009)
  • Author(s): 蛯沢克己
  • Organizer: 2^<nd> TERMIS World
  • Place of Presentation: Seoul
  • Year and Date: 2009-09-01
• [Presentation] Clinical Experience of Cosmetic Regenerative Medicine for Facia 1 Anti-Aging. (2009)
  • Author(s): 蛯沢克己、加藤竜司、岡田真衣、亀井譲、山本朗仁、各務秀明、上田実
  • Organizer: 2^<nd> Annual Meeting of International Society of Tissue Engineering and Regenerative Medicine
  • Place of Presentation: Lotte World Hotel ソウル、韓国
  • Year and Date: 2009-09-01
• [Presentation] 培養線維芽細胞を用いた見た目のアンチエイジング (2009)
  • Author(s): 蛯沢克己、加籐竜司、岡田真衣、成田裕司、上田実
  • Organizer: 第9回日本抗加齢医学会
  • Place of Presentation: 日航ホテル東京台場、東京
  • Year and Date: 2009-05-29
• [Presentation] Cell Therapy for Facial Anti-Aging Using Cultured Autologous Gingival Fibroblasts. (2009)
  • Author(s): 蛯沢克己、加藤竜司、岡田真衣、各務秀明、上田実
  • Organizer: 第2回日韓美容外科学会
  • Place of Presentation: COEX Congress Centerソウル、韓国
  • Year and Date: 2009-04-19
• [Presentation] Cell therapy for facial anti-aging using cultured autologous gingival fibroblasts. (2008)
  • Author(s): Ebisawa K, Kato R, Okada M, Kamei Y, Latif MA, Narita Y, Kagami H, Ueda M
  • Organizer: TERMIS-AP
  • Place of Presentation: 台北
  • Year and Date: 2008-11-07
• [Presentation] Cell therapy for facial ati-aging using cultured autlogous gingival fibroblasts (2008)
  • Author(s): Katsumi Ebisawa
  • Organizer: 2008 Annual Conference of Tissue Engineering and Regenerative Medicine International Society-Asian Pacific Region
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-11-07
• [Presentation] Cell Therapy for Anti-aging (2008)
  • Author(s): Katsumi Ebisawa
  • Organizer: 2^<nd> Malaysian Tissue Engineering & Regenerative Medicine Scientific Meeting
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2008-07-23
• [Book] 抗加齢医療 (2010)
  • Author(s): 蛯沢克己、上田実
  • Total Pages: 220
  • Publisher: 新興出版社
• [Book] 抗加齢医療 (2010)
  • Author(s): 蛯沢克己、上田実
  • Total Pages: 220
  • Publisher: 新興医学出版社
• [Patent(Industrial Property Rights)] 皮膚組織改善材およびその製造方法 (2008)
  • Inventor(s): 蛯沢克己
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2008-06-28
  • Overseas