| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
From comparison of expression profiles of genes in dermal and gingival fibroblasts, only 5% showed a significant difference in the levels of expression. Hierarchical clustering of total genes showed that the difference between gingival and dermal fibroblasts was larger than the difference between ages or gender of patients. Wound closure was significantly accelerated in the wells treated with conditioned medium from gingival fibroblasts than in wells treated with dermal fibroblast conditioned medium, suggesting the presence of functional factor(s) in the conditioned medium that accelerate cell motility/growth of keratinocytes. These results should provide the possibility of effective treatment, which could be genetically designed to provide a higher therapeutic effect by selecting the origin of fibroblasts since gingival and dermal fibroblasts show some unique characteristics in their gene expression profiles.
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